Nanobots were labelled with [18F]F-PyTFP, on the basis of a previously established procedure with minor modifications33 (link). In brief, 200 µl of nanobot solution (1 mg ml−1) was centrifuged (10 min, 13,853g), resuspended in 10 µl of PBS (1 mM, pH 8), and incubated with 4 µl of [18F]F-PyTFP in acetonitrile (about 37 MBq) for 35 min at room temperature. After incubation, the reaction mixture was diluted with water (200 µl) and purified by centrifugation (5 min, 13,853g). The resulting pellet was then rinsed three times with water before being measured in a dose calibrator (CPCRC-25R, Capintec). Radiochemical yield was calculated as the ratio between the amount of radioactivity present in the nanobots after washing and the initial amount of radioactivity. Radiochemical purity after purification was ≥99%, as determined by radio thin-layer chromatography (radio-TLC) using iTLC-SG chromatography paper (Agilent Technologies) and dichloromethane and methanol (2:1) as the stationary and mobile phases, respectively. TLC plates were analysed using a TLC reader (MiniGITA, Raytest).
Cpcrc 25r
Manufactured by Mirion Technologies
Sourced in United States
The CPCRC-25R is a laboratory equipment product from Mirion Technologies. It is a compact, portable unit designed for radiation protection and contamination control. The core function of the CPCRC-25R is to monitor and measure radiation levels in controlled environments.
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Lab products found in correlation
4 protocols using cpcrc 25r
1
Radiolabeling Nanobots with [18F]F-PyTFP
2
Radiolabeling of Urease-Functionalized Gold Nanoparticles
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The radioiodination of urease-AuNPs was performed by incubation with [ 124 I]NaI. Briefly, 200 l of urease-AuNP solution (1 mg/ml) diluted in 100 l of water and 8 l of [ 124 I]NaI (about 1L MBq) were incubated at room temperature for 30 min. After incubation, the reaction mixture was purified by centrifugation (5 min, 14,800 rpm).
The resulting precipitate was washed three times with water (100 l). The amounts of radioactivity in the supernatant and the precipitate were determined in a dose calibrator (CPCRC-25R, Capintec Inc., NJ, USA) and analyzed with radio-TLC, using an iTLC-SG chromatography paper (Agilent Technologies, CA, USA) and dichloromethane and methanol (2:1) as the stationary and mobile phases, respectively. TLC plates were analyzed using a TLC reader (MiniGITA, Raytest).
The resulting precipitate was washed three times with water (100 l). The amounts of radioactivity in the supernatant and the precipitate were determined in a dose calibrator (CPCRC-25R, Capintec Inc., NJ, USA) and analyzed with radio-TLC, using an iTLC-SG chromatography paper (Agilent Technologies, CA, USA) and dichloromethane and methanol (2:1) as the stationary and mobile phases, respectively. TLC plates were analyzed using a TLC reader (MiniGITA, Raytest).
3
Radioiodination of Gold Nanomotors
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The radioiodination of the gold nanomotors was performed by incubation with [ 124 I]NaI. In a typical experiment, 50 µL of gold nanomotors solution (1 mg/mL) and 30 µL of [ 124 I]NaI (approximately 15 MBq) were incubated at 25°C for 10 minutes. After incubation, the crude material was purified by centrifugation (10 min, 13400 rpm). The resulting precipitate was washed three times with 1% SDS solution to remove unreacted 124 I species, and the amount of radioactivity in the pellet, the supernatant and the washings were determined in a dose calibrator (CPCRC-25R, Capintec Inc., NJ, USA). Finally, the gold micromotors were resuspended in 1% SDS solution (100 µL). Radiochemical yield (expressed in percentage) was calculated as the ratio between the amount of radioactivity in the resuspended fraction and the starting amount of radioactivity.
4
Radiolabeled Nanomotor Stability Testing
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The radiochemical stability of the radiolabelled nanomotors was assessed by incubation in 1% SDS solution, SDS (1.0 % w/v)/H2O2(1.5 % v/v) solution, and rat and mouse plasma. At different time points (10, 20, 30, 40 and 60 min and 24 hours), samples were withdrawn, and the amount of radioactivity was measured. After incubation, the nanomotors were separated by centrifugation (10 min, 13400 rpm). The resulting precipitate was washed twice with 1% SDS solution, and the amount of radioactivity in the pellet, the supernatant and the washings were determined in a dose calibrator (CPCRC-25R, Capintec Inc., NJ, USA). The radiochemical stability was calculated as the percentage of radioactivity in the pellet with respect to the total amount of radioactivity (pellet + filtrate + washings).
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