Immunoblotting was performed as previously reported16 (link),17 (link). The antibodies used were mouse monoclonal anti-VDAC1 (ab14734), mouse monoclonal anti-SDHA (ab14715), mouse monoclonal anti-Rieske (ab14746), mouse monoclonal anti-SIRT1 (ab110304), rabbit polyclonal anti-histone H3 (ab1791) from Abcam (Cambridge, UK); mouse monoclonal anti-HSP60 (SPA-806) from Enzo Life Sciences (Farmingdale, NY); guinea pig polyclonal anti-p62 (GP62-C) from Progen (Heidelberg, Germany); rabbit monoclonal anti-LC3A/B (#12741), rabbit monoclonal anti-phospho-Ser37/46-4EBP1 (#2855), rabbit polyclonal anti-4EBP1 (#9452), rabbit polyclonal anti-phospho-ser235/236 S6 (#2211), rabbit monoclonal anti-S6 (#2217), rabbit polyclonal anti-phospho-Ser473 Akt (#9271), rabbit polyclonal anti-Akt (#9272), rabbit monoclonal anti-FoxO3a (#2497), Lamin A/C (#2032) from Cell Signaling Technologies (Danvers, MA, USA); mouse monoclonal anti-GAPDH (G8795), mouse monoclonal anti-α-tubulin (T5168) from Sigma Aldrich (St. Louis, MO, USA); and rabbit polyclonal anti-acetylated histone H3 (382158) from Millipore (Billerica, MA, USA).
Mouse monoclonal anti sirt1 ab110304
Manufactured by Abcam
Sourced in United Kingdom
Mouse monoclonal anti-SIRT1 (ab110304) is a primary antibody that recognizes the SIRT1 protein. SIRT1 is a NAD-dependent deacetylase involved in various cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Mouse monoclonal anti α tubulin t5168, by Merck Group (1 mentions)
Rabbit polyclonal anti akt, by Cell Signaling Technology (1 mentions)
Rabbit monoclonal anti s6, by Cell Signaling Technology (1 mentions)
Lamin a c, by Cell Signaling Technology (1 mentions)
Fluorescein isothiocyanate (fitc), by Jackson ImmunoResearch (1 mentions)
2 protocols using mouse monoclonal anti sirt1 ab110304
1
Immunoblotting Antibody Validation Protocol
2
Antibodies for Oxidative Stress Analysis
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The antisera used for Western blot and immunohistochemistry were: mouse monoclonal anti-SIRT1 (ab110304) and rabbit monoclonal anti-p53 acetyl K370 (ab183544) from Abcam; mouse monoclonal anti-8-OHdG (12501) from QED Biosciences: rabbit polyclonal anti-γH2AX (A300-081A) from Bethyl Laboratories;rabbit polyclonal anti-p53 (#9282) from Cell Signaling Technology; rabbit polyclonal anti-Bax (sc-526) from Santa Cruz Biotechnology; rabbit polyclonal anti-ZO-1 (AB2272) and anti-MnSOD (06-984) from Merck Millipore; mouse monoclonal anti-Bcl2 (B9804),mouse monoclonal anti-catalase (C0979), rabbit polyclonal anti-CX43 (C6219) and mouse monoclonal anti-αtubulin (T6199) from Sigma-Aldrich; biotinylated goat anti-mouse IgG (IgG-B, sc-2039) from Santa Cruz Biotechnology; fluorescein isothiocyanate (FITC) and tetramethylrhodamine-5-(and 6)-isothiocyanate (TRITC) conjugated secondary antibodies from Jackson ImmunoResearch.
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