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Lactobionate

Manufactured by Merck Group
Sourced in United States

Lactobionate is a laboratory chemical compound used as a buffer solution and electrolyte. It is a salt of lactobionic acid, which is derived from the oxidation of lactose. Lactobionate is commonly used in various laboratory applications, including cell culture media, biochemical assays, and pharmaceutical formulations. Its core function is to maintain pH and osmotic balance in these applications.

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2 protocols using lactobionate

1

Cardiac Biomarkers Measurement Protocols

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The main reagents used in the present study are listed below. The cardiac troponin I (cTn-I) and N terminal pro B type natriuretic peptide (NT-proBNP) enzyme-linked immunoassay kits were obtained from Cloud-Clone Corp. (Wuhan, China). ADP potassium salt, Cytochrome c, Lactobionate, ATP Na2, Na2Phosphocreatine, EGTA, Ascorbic acid, Taurine, Imidazole, DTT, HEPES, MES, Glutamate, Malate, Succinate, TMPD, Antimycin A and Rotenone were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The following main instruments were used in the present study: An animal treadmill (Taimeng, China), a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, USA), a pressure volume catheter (SPR-838, Millar Company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), a needle electrode (AD Instruments, Australia) and a high-resolution respirometry (Oroboros Instruments, Austria).
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2

Cardiac Biomarker Detection Assay

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The main reagents used in the present study are listed below. The cardiac troponin I (cTnI) and N-terminal pro B type natriuretic peptide (NT-proBNP) enzyme-linked immunoassay kits were obtained from Shanghai Kmaels Biologic Technology Co., Ltd., ADP potassium salt, cytochrome c, lactobionate, ATP-Na2, Na2Phosphocreatine, EGTA, ascorbic acid, taurine, imidazole, DTT, HEPES, MES, glutamate, malate, succinate, TMPD, antimycin A and rotenone were purchased from Sigma (USA). The following main instruments were used in the present study: an animal treadmill (Taimeng, China), a PowerLab signal acquisition and analysis system, a MultiscanGO enzyme standard instrument (Thermo, USA), a pressure volume catheter (SPR-838, Millar Company, USA), a PowerLab data acquisition and analysis system (AD Instruments, Australia), a bioelectric amplifier (AD Instruments, Australia), a needle electrode (AD Instruments, Australia) and a high-resolution respirometry (Oroboros Instruments, Austria).
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