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3 protocols using d xylose

1

Quantification of Carbohydrate Derivatives

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HPLC-grade d(+)-glucose, d(+)-galactose, d(+)-xylose, l(+)-arabinose, d(+)-mannose, formic acid, acetic acid and furfural were from Panreac (Barcelona, Spain). Levulinic acid was from Fluka Analytical-Sigma Aldrich (Steinheim, Germany). 5-Hydroxymethyl-2-furaldehyde (HMF) and l(−)-fucose were from Sigma Aldrich (Steinheim, Germany). Sodium hydroxide pellets and sulphuric acid were from Panreac (Barcelona, Spain).
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2

Analytical Protocol for Phenolic Compounds

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The water used was Milli-Q water (Millipore Corporation, Molsheim, France) with a conductivity of 18 MΩ cm–1. High-performance liquid chromatography (HPLC)-grade methanol and formic acid were used as the solvents for the HPLC analysis and were obtained from Sigma-Aldrich. Ethanol (99.5%) was obtained from VWR (Fontenay-sous-Bois, France). The phenolic standards, cyanidin chloride, malvidin chloride, delphinidin chloride, p-coumaric acid, ellagic acid, resveratrol, gallic acid, caffeic acid, polydatin, epicatechin gallate, epicatechin, vanillin, and vanillic acid, were purchased from Extrasynthese (Genay, France). Bovine serum albumin (BSA) was purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). Tartaric acid, NaN3, and NaNO3, p.a. grade, were from Merck (Darmstadt, Germany). Folin–Ciocalteu reagent was purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.), and sodium carbonate was purchased from Merck (Darmstadt, Germany). HCl was purchased from VWR International (Rue Carnot, France). The sugars used as standards were l(+)-rhamnose monohydrate, d(+)-galactose, and d(+)-glucose from Merck, l(+)-arabinose from VWR Chemicals, d(+)-xylose from PanReac AppliChem (Darmstadt, Germany), and d(+)-mannose from Sigma-Aldrich.
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3

Comprehensive Reagent Inventory for Research

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All the chemicals used in this work were acquired in reagent grade or were suitable for cell culture. Agar (bacteriological), BCA Protein Assay Kit, d-(+)-Maltose monohydrate (95 %), l-(+)-Arabinose (99 %), peptone (bacteriological), and yeast extract were supplied by Thermo Fisher Scientific (USA). d-(+)-Xylose (98.5 %), Folin-Ciocalteu's reagent, methanol (99 %), sulfuric acid (98 %), and sodium acetate anhydrous (99.9 %) were obtained from PanReac AppliChem (Barcelona, Spain). Acetic acid (99.7 %), ammonium sulfate (99 %), 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS), calcium chloride (99.9 %), copper (II) sulfate anhydrous (99.9 %), gallic acid (97.5 %), l-(-)-Malic acid (99 %), l-(+)-tartaric acid (99 %), magnesium sulfate heptahydrate (99 %), potassium chloride (99 %), potassium phosphate monobasic (99 %), sodium selenite (98 %), succinic acid (99 %), thiamine hydrochloride (99 %), and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (97 %) were purchased from Merck (Darmstadt, Germany). Glucose POD-GOD kit was obtained from Spinreact (Sant. Esteve de Bas, Girona, Spain). Citric acid (99.5 %), d-(+)-Glucose (97.5 %), and malt extract agar were supplied by Scharlau (Barcelona, Spain).
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