Anthropometric measurements including weight and height were obtained using standardized techniques. The body mass index (BMI) was calculated as the weight in kilograms divided by the square of height in meters. Biochemical analyses were carried out on a Hitachi-912 Autoanalyzer (Hitachi, Mannheim, Germany) using commercial kits (Roche Diagnostics, Mannheim, Germany). Fasting plasma glucose was estimated using glucose oxidase-peroxidase method. Serum cholesterol was estimated using cholesterol oxidase-phenol 4-amino antipyrene peroxidase (CHOD-PAP) method. Serum triglyceride was estimated using glycerol phosphatase oxidase-PAP (GPO-PAP) method. High-density lipoprotein (HDL) cholesterol was estimated using polyethylene glycol-pretreated enzymes method and low-density lipoprotein (LDL) cholesterol was calculated using Friedewald formula. Glycated hemoglobin (HbA1C) was estimated by high performance liquid chromatography using the Variant machine (Bio-Rad, Hercules, USA). Serum insulin concentration was estimated using Dako kits (Dako, Glostrup, Denmark).
Dako kits
Manufactured by Agilent Technologies
Sourced in Denmark
Dako kits are a line of laboratory equipment and reagents designed for use in immunohistochemistry and in situ hybridization applications. The kits provide the necessary tools and materials for the detection and visualization of specific proteins or genetic sequences within tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using dako kits
1
Anthropometric and Biochemical Measurements
2
Immunohistochemical Detection of Mismatch Repair Genes
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Four primary antibodies were used to detect each of MSH6, MSH2, MLH1 and PMS2 by using Dako kits to detect some of the mutated genes immunohistochemically. According to the manufacturer's instructions. The primary antibodies were applied on 5 µm formalin-fixed paraffinembedded (FFPE).
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