Proteins were lysed in radioimmunoprecipitation assay buffer containing protease inhibitors (Beyotime Biotechnology), separated by electrophoresis and transferred to PVDF membranes (Bio‐Rad). After overnight incubation with primary Abs (anti‐METTL3 [1:1000 dilution, ab195352; Abcam], anti‐CTSL [1:500 dilution, 66,914‐1‐lg; Proteintech], anti‐insulin‐like growth factor 2 mRNA‐binding protein [IGF2BP2] [1:1000 dilution, ab128175; Abcam], anti‐METTL14 [1:1000 dilution, ab220030’ Abcam], anti‐RNA binding motif protein 15 [RBM15] [1:1000 dilution; PTM‐6163; Jingjie PTM BioLab], and anti‐β‐actin [1:5000 dilution; 4970S; Cell Signaling Technology]), the membranes were incubated with a HRP‐conjugated secondary Ab (1:5000 dilution, 7076S, 7074S; Cell Signaling Technology). The immunoreactions were visualized using the ECL Plus substrate (Merck Millipore). β‐Actin was used for normalization.
Anti ctsl
Manufactured by Proteintech
Anti-CTSL is a primary antibody that detects the CTSL (Cathepsin L) protein. CTSL is a lysosomal cysteine protease involved in intracellular protein degradation. This antibody can be used for Western blotting, immunohistochemistry, and other immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab128175, by Abcam (1 mentions)
Protease inhibitor, by Beyotime (1 mentions)
Anti mettl3, by Abcam (1 mentions)
Anti mettl14, by Abcam (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Anti mmp9, by Proteintech (1 mentions)
Anti bcl 2, by Proteintech (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Anti bax, by Proteintech (1 mentions)
2 protocols using anti ctsl
1
Western Blot Analysis of RNA-Binding Proteins
2
Inhibition of CTSL Induces Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CTSL inhibitor 1 was purchased from Selleck Chemicals (Shanghai, China). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). PE Annexin V apoptosis detection commercial kit were purchased from BD Biosciences (Shanghai, China). Cell-Light EdU Apollo567 in Vitro Kit was purchased from Guangzhou Ruibo Biotechnology Co. LTD (Guangzhou, China). Anti-CTSL, anti-Bax, anti-Bcl-2 and anti-MMP-9 antibodies were obtained from Proteintech (Wuhan, China). Anti-GAPDH was purchased from Abcam (Cambridge, UK).
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