S. pneumoniae D39 and D39-pEVP3-fstW containing FtsW::LacZ fusion were cultured in C+Y medium until OD600 = 0.5–0.6. Subsequently, 107 CFU bacteria were collected by centrifugation at 13,800 g for 2 min. The pellet was collected and washed twice with PBS. The pellet was resuspended in 500 μL of 0.1% triton X-100 dissolved in PBS and then incubated for 15 min at 37°C. 50 μL of the reaction product was used for detection following the instructions of β-galactosidase reporter gene assay kit (Beyotime, China). Finally, the protein expressions were detected using a micro plate reader at 450 nm.
β galactosidase reporter gene assay kit
Manufactured by Beyotime
Sourced in China
The β-galactosidase reporter gene assay kit is a laboratory tool used to measure the activity of the β-galactosidase enzyme. The kit provides the necessary reagents and protocols to quantify the expression of genes that are fused to the β-galactosidase reporter gene.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using β galactosidase reporter gene assay kit
1
FtsW Fusion Protein Localization
2
ADAM10 Promoter Luciferase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
NIH3T3 cells were transfected with 1 μg pGL3 with ADAM10 promoter controlled luciferase gene and 1 μg pSV-β-galactosidase using 4 μl Fugene or X-tremeGENE 9 in Opti-MEM (Invitrogen). After 12 hrs CT-1 stimulation, the media were changed and cells were lysed for measurement of luciferase activity using a luciferase assay system (Promega E1483) and galactosidase activity with β-galactosidase Reporter gene assay kit (Beyotime RG0036). Luciferase activity normalized to β-galactosidase activity represents the promoter activity.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!