For FACS of B cells, PBMC were stained with BUV395 anti-CD19 (BD Biosciences), APC anti-CD27 (Biolegend) and either PE anti-CXCR3 (eBioscience), PE-Cy7 anti-CXCR5 (eBioscience), PE anti-CD11a (Biolegend), PE anti-CD49d (Biolegend), PE anti-CD166 (BD) or FITC anti-CD6 (Biolegend). BUV395 anti-CD19 APC anti-CD27-stained cells were also fixed and permeabilized and stained with rabbit anti-AHNAK (Sigma) and PE donkey anti-rabbit IgG. FCS files of stained cells were collected on a LSRII cytometer (BD) and analyzed using FloJo.
Fitc anti cd6
Manufactured by BioLegend
FITC anti-CD6 is a fluorescently-labeled monoclonal antibody that binds to the CD6 cell surface receptor. CD6 is expressed on T cells and a subset of B cells, and is involved in T cell activation and adhesion. The FITC (fluorescein isothiocyanate) label allows for the detection and identification of CD6-positive cells using flow cytometry or other fluorescence-based techniques.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd166 pe, by BD (2 mentions)
Buv395 anti cd19, by BD (2 mentions)
Anti cd27 apc, by BioLegend (2 mentions)
Anti cd49d pe, by BioLegend (2 mentions)
Lsrii cytometer, by BD (2 mentions)
Pe anti cd11a, by BioLegend (2 mentions)
Pe anti cxcr3, by Thermo Fisher Scientific (2 mentions)
Pe cy7 anti cxcr5, by Thermo Fisher Scientific (2 mentions)
Buv395 anti cd19 apc anti cd27, by Merck Group (2 mentions)
Rabbit anti ahnak, by Merck Group (2 mentions)
2 protocols using fitc anti cd6
1
FACS Analysis of B Cell Subsets
2
FACS Analysis of B Cell Subsets
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For FACS of B cells, PBMC were stained with BUV395 anti-CD19 (BD Biosciences), APC anti-CD27 (Biolegend) and either PE anti-CXCR3 (eBioscience), PE-Cy7 anti-CXCR5 (eBioscience), PE anti-CD11a (Biolegend), PE anti-CD49d (Biolegend), PE anti-CD166 (BD) or FITC anti-CD6 (Biolegend). BUV395 anti-CD19 APC anti-CD27-stained cells were also fixed and permeabilized and stained with rabbit anti-AHNAK (Sigma) and PE donkey anti-rabbit IgG. FCS files of stained cells were collected on a LSRII cytometer (BD) and analyzed using FloJo.
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