Two hundred consecutive isolates of A. baumannii collected from clinical samples between June 2016 and January 2017 were included in the study. Only the first isolate from each patient was accepted. Bacterial identification was confirmed with Vitek 2 ID/AST (bio-Mérieux, France) automated bacterial identification system and MALDI-TOF MS (bio-Mérieux). Isolates were stored in brain-heart infusion broth containing 10% glycerol at -20 °C until the day of study.
Vitek 2 id ast
Manufactured by bioMérieux
Sourced in France
The Vitek 2 ID/AST is a compact, fully automated microbiology system designed for the rapid identification and antimicrobial susceptibility testing of a wide range of clinically significant microorganisms. It utilizes advanced technology to provide accurate and reliable results to support clinical decision-making.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using vitek 2 id ast
1
Clinical Isolates of Acinetobacter baumannii
2
Urine Sample Processing and Bacterial Identification
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The processing of urine samples arriving at the Department of Medical Microbiology during the study period was carried out based on the relevant guidelines published by the Hungarian Ministry of Health [35 ]. Identification of isolates was carried out via classical biochemical tests, the VITEK 2 ID/AST (bioMérieux, Marcy-l’Étoile, France), and after 2012, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) using a MicroFlex MALDI Biotyper (Bruker Daltonics, Bremen, Germany) [36 (link)]. Antimicrobial susceptibility testing for Gram-negative bacteria and the interpretation of the results was performed based on the recommendations of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) at the time of isolation, taking into account the intrinsic resistance mechanisms of isolated bacteria [37 ]. Resistance to third-generation cephalosporins (3GCs) in Enterobacterales species was inferred from resistance against ceftriaxone and/or ceftazidime [38 ]. Intermediate results (I) for antibiotics other than 3GCs were grouped with and reported as resistant (R) [36 (link)].
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