In Western blotting of non-denaturing PAGE, the monoclonal antibody rH02 (5 ug/ml) was used for H-ferritin detection in the heteropolymer. For the detection of L-ferritin, SDS-Page was used with the polyclonal rabbit anti-ferritin antibody (Sigma) that preferentially recognizes the L-chains. Antibody binding was revealed using horseradish peroxidase-labeled secondary antibody and ECL detection. Coomassie Brillant Blue staining was used to verify the loading of both non-denaturing and SDS-PAGE.
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Rabbit anti ferritin antibody
Manufactured by Merck Group
Sourced in United Kingdom
The Rabbit anti-ferritin antibody is a laboratory reagent used to detect and quantify the presence of ferritin, an iron-storage protein, in biological samples. This antibody is produced by immunizing rabbits with ferritin and purifying the resulting antibodies. It can be used in various immunoassay techniques, such as ELISA, to measure ferritin levels in research and clinical settings.
Automatically generated - may contain errors
2 protocols using rabbit anti ferritin antibody
1
Western Blotting of Ferritin Isoforms
2
Protein Extraction and Western Blotting
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Protein extraction and Western Blotting were performed as described [51 (link)]. Used antibodies were a rabbit anti-Lcn2 antibody (1:1000; abcam, Cambridge, United Kingdom), a mouse anti-TFR1 antibody (1:1000; Sigma Aldrich), a rabbit anti-ferritin antibody (1:500; Sigma), a rabbit anti-Ho1 antibody (1:1000; abcam), a rabbit anti-Irp2 antibody (1:1000; Novusbio, Littleton, CO, USA) and a rabbit actin antibody (1:500; Sigma Aldrich), and appropriate HRP-conjugated secondary antibodies (1:2000, anti-rabbit; 1:4000, anti-mouse; Dako, Glostrup, Denmark). For quantification, densitometry data were acquired on a ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) and analyzed with Image Lab 5.2.1. (Bio-Rad).
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