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Pe conjugated cd133 1 ac133 antibody

Manufactured by Miltenyi Biotec
Sourced in Germany

The PE-conjugated CD133/1 (AC133) antibody is a laboratory reagent used for the detection and identification of CD133-positive cells. CD133 is a surface antigen that is expressed on various types of stem and progenitor cells. The PE-conjugation allows for the fluorescent labeling of CD133-positive cells, enabling their visualization and analysis using flow cytometry or other fluorescence-based techniques.

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2 protocols using pe conjugated cd133 1 ac133 antibody

1

CD133 Expression in Liver Cancer Cells

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The PLC/PFR/5 and Huh7 cells were incubated with PE-conjugated CD133/1 (AC133) antibody (Miltenyi Biotec, Germany), and the percentage of CD133+ cells was detected using an Epics Altra flow cytometer (Beckman Coulter, USA). For MHCC-97L and PLC/PFR/5 cells, the CD133+ and CD133 cells were magnetically isolated using corresponding antibodies and an EasySep PE Selection Kit (Stem Cell Technologies) according to the manufacturer's instructions. The purity of the sorted cells was evaluated by western blot.
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2

CD133+ Cell Sorting from Liver Cancer Cell Lines

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Huh7, PLC/PRF/5, HCC-LY5, and SMMC-7721 cells were incubated with a PE-conjugated CD133/1(AC133) antibody (Miltenyi Biotec, Germany) and sorted into CD133+ and CD133 cell subpopulations on an Epics Altra flow cytometer (Beckman Coulter, USA)
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