Phospho 4e bp1

Manufactured by Abcam

Phospho-4E-BP1 is a lab equipment product that detects the phosphorylation of 4E-BP1, a key regulator of protein synthesis. It is used to monitor the activity and regulation of this important cellular process.

Automatically generated - may contain errors

Lab products found in correlation

Tunicamycin, by Merck Group (1 mentions) Sc 7160, by Santa Cruz Biotechnology (1 mentions) Antibody against α tubulin, by Merck Group (1 mentions) Rapamycin, by Selleck Chemicals (1 mentions) Sc 200, by Santa Cruz Biotechnology (1 mentions) Nbp1 40256, by Novus Biologicals (1 mentions) Sc 8327, by Santa Cruz Biotechnology (1 mentions) Sc 575, by Santa Cruz Biotechnology (1 mentions) Bip 610978, by BD (1 mentions) Phospho s6, by Abcam (1 mentions) Phospho h2ax, by Abcam (1 mentions) Ascl1, by BD (1 mentions)

2 protocols using phospho 4e bp1

Studying ER Stress Signaling Pathways

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Murine recombinant insulin-like growth factor-1 (IGF-1) was purchased from PeproTech (Rocky Hill, NJ). Thapsigargin was obtained from Applichem (St Louis, MO), tunicamycin from Sigma (St Louis, MO) and brefeldin A from TOKU-E (Bellingham, WA). mTOR and PERK inhibitors were purchased from LC Labs (Woburn, MA) (rapamycin), Selleckchem (Houston, TX) (WYE125132, GSK2656157), Abcam (Cambridge, UK) (PP242) and from GlaskoSmith and Kline (Middlesex, UK) (GSK2126458). Antibodies against phospho-PERK (Thr980) (No.3179), PERK (No. 3192), phospho-eIF2α (Ser51) (No. 3597), eIF2α (No.2103), phospho-p70S6K1^thr389 (No. 9234), p70S6K1 (No. 9202) and cleaved-caspase3 (No. 9664) were purchased from Cell Signaling Technology (Beverly, MA). Phospho-4E-BP1 (phosphorylation on Thr45; ab68187), and 4E-BP1 (ab2606) were from Abcam (Cambridge, UK). ATF6 clone 70B1413.1 were from Abcam (Cambridge, UK; ab11909) and Novus Biological (Littleton, CO; NBP1-40256). Antibodies against CHOP (SC-575), ATF4 (SC-200), GADD34 (SC-8327) and XBP1 (SC-7160) came from SantaCruz Biotechnology (Santa Cruz, CA) and BiP (610978) from BD Laboratories^™ (Franklin Lakes, NJ). Antibody against α-tubulin was from Sigma-Aldrich (St. Louis, MO).

Freis P., Bollard J., Lebeau J., Massoma P., Fauvre J., Vercherat C., Walter T., Manié S., Roche C., Scoazec J.Y, & Ferraro-Peyret C. (2017). mTOR inhibitors activate PERK signaling and favor viability of gastrointestinal neuroendocrine cell lines. Oncotarget, 8(13), 20974-20987.

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Immunoblot Primary Antibody Panel

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Primary antibodies for immunoblot include: MYC (CST #13987, 1:1,000), MYCN (Santa Cruz sc-791, 1:200), ODC1 (EMD Millipore, MABS36, 1:100), ASCL1 (BD Bioscience, BDB556604, 1:300), phospho-4EBP1 (CST #2855, 1:1,000), 4EBP1 (CST #9644, 1:2,000), phospho-S6 (CST #2211, 1:1,000), S6 (CST #2217, 1:1,000), phospho-H2AX (CST #9718, 1:1000), PARP (CST #9532, 1:1000), ASL (Abcam#201026), ASS1 for mouse tumors (Abcam #170952, 1:1,000), ASS1 for human PDX and cell lines (Polaris Pharmaceuticals, 1:1000), and HSP90 (CST #4877, 1:1,000).

Chalishazar M.D., Wait S.J., Huang F., Ireland A.S., Mukhopadhyay A., Lee Y., Schuman S.S., Guthrie M.R., Berrett K.C., Vahrenkamp J., Hu Z., Kudla M., Modzelewska K., Wang G., Ingolia N.T., Gertz J., Lum D.H., Cosulich S.C., Bomalaski J., DeBerardinis R.J, & Oliver T.G. (2019). MYC-driven small cell lung cancer is metabolically distinct and vulnerable to arginine depletion. Clinical cancer research : an official journal of the American Association for Cancer Research, 25(16), 5107-5121.

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