Hp molecular sieve 5 plot capillary column

AVOXimeter 4000 Whole Blood CO-Oximeter from ITC was used for all COHb analyses. Manufacturer guidelines were followed to obtain COHb concentrations.
Agilent 6890 N GC (Palo Alto, USA) equipped with a HP Molecular Sieve 5 Å PLOT capillary column (30 m x 0.32 mm x 30µm) purchased from Restek (Bellefonte, USA) was used for gas chromatographic analysis. The temperature program used was as follows: 50°C, held for 4 minutes; injector temperature was set at 180°C, the injector used in splitless mode, and the MS interface at 230°C. Helium was employed as carrier gas, at a flow rate of 40 mL/min. An Agilent 5973 mass spectrometer (Palo Alto, USA) was used for detection, operating in electron ionization (EI) mode at 70eV. Selected Ion Monitoring (SIM) mode was used to acquire the signal for CO at m/z 28 and 13 CO at m/z 29.

For spectrophotometric analysis, AVOXimeter 4000 Whole Blood CO-Oximeter from ITC was used. Manufacturer guidelines were followed to obtain COHb analyses.
For gas chromatographic analysis, Agilent 6890 N GC (Palo Alto, USA) equipped with a HP Molecular Sieve 5 Å PLOT capillary column (30 m x 0.32 mm x 30µm) obtained from Restek (Bellefonte, USA) was used. Following temperature programme was used: 50°C, held for 4 minutes; the injector was set at 180°C, used in splitless mode, and the MS interface at 230°C. The employed carrier gas was helium at a flow rate of 40.0 mL/min. A solvent delay of 1.8 minutes was used.
For detection, Agilent 5973 mass spectrometer (Palo Alto, USA) was used, operating in electron ionization (EI) mode at 70eV. Selected Ion Monitoring (SIM) mode was used to acquire the signal for CO at m/z 28 and 13 CO at m/z 29.