Yeast extract, yeast nitrogen base and peptone were purchased from Lab M Limited (UK), whereas all other chemicals were reagent grade and purchased from Sigma Aldrich unless otherwise specified. Locust bean galactomannan was purchased from Fluka, whereas galactoxyloglucan from tamarind seed was obtained from Megazyme, and guar galactomannan was obtained from Sigma Aldrich (batch 14K0168). Spruce galactoglucomannan was generously provided by Professor Stefan Wilför (Åbo Academy, Finland), and Pichia pastoris KM71H expressing wild-type GaO was kindly supplied by Professor H. Brumer (University of British Columbia) [26] (link). Reported molecular weights of polysaccharides used in this analysis are as follows: 29 kDa for spruce galactose glucomannan [27] (link), 470 kDa for tamarind galactoxyloglucan, 2600 kDa for guar galactomannan [7] (link), and 310 kDa for Locust bean galactomannan (as stated by Sigma-Aldrich (G0753)).
Mollerup F., Parikka K., Vuong T.V., Tenkanen M, & Master E. (2016). Influence of a family 29 carbohydrate binding module on the activity of galactose oxidase from Fusarium graminearum. Biochimica et biophysica acta, 1860(2).
+ Open protocol