ICH was induced by a double-injection method described previously, 29 (link) with minor modifications. Briefly, 30-μL nonheparinized blood was withdrawn from the angular vein after anesthesia and quickly transferred into a 50-μL syringe with a 26 G needle (Hamilton Company). Then mouse was fixed on a stereotactic frame (RWD Life Science). A 1-mm burr hole was drilled, and blood was infused at the caudate nucleus (2.3 mm lateral to midline, 0.5 mm anterior to bregma, and 3.5mm depth below the surface of the skull). Each ICH model received 30-μL whole blood at a rate of 1 μL/min through the infusion pump (KD Scientific). During the process, needle was paused for 5 minutes before injection, then the first 5 μL was injected to generate a clotting along the needle track, after an additional 5-minute pause, the remaining 25 μL was injected during the following 25 minutes at the same rate of 1 μL/min. After completing the infusion, the needle was held in place for 20 minutes, and then it was slowly withdrawn at a rate of 1 mm/min over the course of 3 movements with 5-minute intervals. Finally, the burr hole was sealed with bone wax (Johnson & Johnson), sterilized and the wound was sutured. After surgery, animals were placed in a cage with free access to food and water. Warming lamps were used to provide thermal support throughout the procedure.
26 g needle
Manufactured by Hamilton Company
The 26 G needle is a medical device used for various procedures. It is a thin, hollow needle designed for precise and minimally invasive applications. The core function of the 26 G needle is to provide a small-diameter entry point for delivery or extraction of fluids, medications, or other substances as required by the intended medical procedure.
Automatically generated - may contain errors
2 protocols using 26 g needle
1
Intracerebral Hemorrhage Mouse Model
2
Orthotopic Xenograft Model of Brain Tumors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Permission for animal experiments were obtained from the institutional animal care committee for the University Hospital Würzburg. All animal experiments were performed in accordance with national guidelines and regulations and with approval of the district government. Female NOD.CB17-Prkdcscid/J (NOD/SCID) mice were purchased from The Jackson Laboratory and housed under specific pathogen free conditions. Single cell suspensions were prepared either by mechanical disruption or enzymatical detachment, where necessary. Cell numbers were adjusted in culture medium by serial dilution, calculated for an inoculation volume of 3 μl. Cells were orthotopically injected into the brains of 10–13 week-old anesthetized NOD/SCID mice using a stereotaxic instrument (David Kopf Instruments) and a Hamilton syringe with a 26 G needle (Hamilton Company), injecting at defined coordinates: two injection sites were evaluated: for supratentorial inoculation cells were injected in the dorsolateral thalamus, for infratentorial inoculation cells were injected in the right cerebellum. Subsequently, mice were checked daily using bioluminescence imaging (BLI). Survival was defined as the time from transplantation until an early humane endpoint when mice were sacrificed because they showed first symptoms of disease.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!