Thiobarbituric acid (TBA), bovine serum albumin (BSA), Sephadex G-25, and 5,5′ dithiobis 2-nitrobenzoic acid (DTNB) were purchased from Sigma Chemical Co., USA. N′N′-bis-acrylamide and NNN′N′-tetramethylethylenediamine (TEMED) were purchased from Merk-Schudart, and reduced glutathione, ascorbic acid, horse radish peroxidase, hydrogen peroxide, 2,4-dinitrophenylhydrazine (DNPH), guanidine hydrochloride, acrylamide, sodium dodecyl sulfate (SDS), ferric chloride, potassium ferricyanide, and ammonium persulfate were obtained from SISCO Research Laboratory, India. Digitonin was procured from Hi-media Ltd., India. All other chemicals used were of analytical grade.
Sodium dodecyl sulfate (sds)
Manufactured by Sisco Research Laboratories
Sourced in India
Sodium dodecyl sulfate (SDS) is an anionic surfactant commonly used in laboratory applications. It is a detergent that helps solubilize and denature proteins. SDS is often used in biochemical techniques such as gel electrophoresis and Western blotting to separate and analyze proteins.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium persulfate, by Sisco Research Laboratories (4 mentions)
Thiobarbituric acid, by Merck Group (3 mentions)
2 4 dinitrophenylhydrazine, by Sisco Research Laboratories (2 mentions)
Ferric chloride, by Sisco Research Laboratories (2 mentions)
Sodium chloride (nacl), by Sisco Research Laboratories (2 mentions)
Acetic acid, by Sisco Research Laboratories (2 mentions)
Sodium carbonate, by Sisco Research Laboratories (2 mentions)
Casein, by Sisco Research Laboratories (2 mentions)
Bovine serum albumin (bsa), by Sisco Research Laboratories (2 mentions)
Triton x 100, by Merck Group (2 mentions)
Hydrogen peroxide h2o2, by Sisco Research Laboratories (2 mentions)
Trichloroacetic acid, by Sisco Research Laboratories (2 mentions)
Baicalein, by Merck Group (2 mentions)
5 5 dithiobis 2 nitrobenzoic acid, by Merck Group (1 mentions)
Horse radish peroxidase, by Sisco Research Laboratories (1 mentions)
Hydrogen peroxide, by Sisco Research Laboratories (1 mentions)
Acrylamide, by Sisco Research Laboratories (1 mentions)
Potassium ferricyanide, by Sisco Research Laboratories (1 mentions)
Silver nitrate, by Sisco Research Laboratories (1 mentions)
Thiourea, by Sisco Research Laboratories (1 mentions)
9 protocols using sodium dodecyl sulfate (sds)
1
Biochemical Assay Reagents and Protocols
2
Two-Dimensional Gel Electrophoresis Protocol
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Acrylamide, agarose, bovine serum albumin (BSA), bromophenol blue, 3-[(3-cholamidopropyl) dimethylammonio]-1-propanesulfonate (CHAPS), dithiothreitol (DTT), Histopaque (specific gravity 1.077), iodoacetamide (IAA), methylenebisAcrylamide, phenylmethane sulfonyl fluoride (PMSF), and Trizma were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Immobilized pH gradient strips, BioLyte 3/10, mineral, 2-DE cleanup kit, and 2-DE sodium dodecyl sulfate polyAcrylamide gel electrophoresis (SDS-PAGE) marker proteins (cat. no. 161-0320) were purchased from Bio-Rad (Hercules, CA, USA). Acetic acid, acetone, ammonium persulfate, ethanol, glycine, silver nitrate, sodium carbonate, sodium chloride, sodium dihydrogen phosphate, disodium hydrogen orthophosphate, sodium dodecyl sulfate (SDS), sodium thiosulfate, urea, and thiourea were purchased from Sisco Research Laboratories Pvt. Ltd. (Mumbai, India). Glycerol and formaldehyde were procured from Qualigen (Carlsbad, CA, USA). All other chemicals used were of analytical grade.
3
Purification and Characterization of Fibrinogen
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Trypsin I, human fibrinogen, specific protease inhibitors viz., iodoacetic acid (IAA) and phenylmethylsulfonyl fluoride (PMSF) were procured from Sigma-Aldrich Corporation (St. Louis, MO). Trypsin II was purchased from Hi-media Laboratories Pvt. Ltd., (Mumbai, India). All the chemicals like casein, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), sodium dodecyl sulfate (SDS), ammonium persulfate etc., were from Sisco Research Laboratories Pvt. Ltd.(Mumbai, India).
4
Dacarbazine Cytotoxicity Assay Protocol
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Analytical grade dacarbazine (daczin, 200 mg) (Indon, Zydus Cadila Healthcare Ltd., Ahmedabad, India), MEM Media, Tris-HCl, Hydroxylamine hydrochloride (Hi-media Laboratories Ltd., Mumbai, India), nitroblue tetrazolium (NBT), sodium azide, ethylene diamine tetra acetic acid (EDTA), Hydrogen peroxide, Reduced glutathione, 5,5’-dithiobis (2-nitrobenzoic acid) (DTNB), Sodium citrate, Sodium dodecyl sulfate (Sisco Research Laboratory, India), thiobarbituric acid (TBA) and trichloroacetic acid (TCA) (Merck India Ltd, Mumbai, India) were used for study.
5
Biochemical Assays Protocol
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Agarose, 1-chloro-2,4-dinitrobenzene, 2,2-diphenyl-1-picrylhydrazyl (DPPH), N-ethylmaleimide, glutathione reductase (GR), L-leucine p-nitroanilide, metaphosphoric acid, NaNO2, Roswell Park Memorial Institute 1640 (RPMI) medium, Triton X-100, 2,4,6-tris(2-pyridyl)-s-triazine, p-nitrophenyl phosphate, γ-glutamyl p-nitroanilide, o-phthalaldehyde and NaNO2 were from Sigma Aldrich, USA. Bovine serum albumin, diphenylamine, 5,5'-dithiobisnitrobenzoic acid (DTNB), ethylenediaminetetraacetic acid (EDTA), glucose 6-phosphate, proteinase K, reduced (GSH) and oxidized (GSSG) glutathione, hydrogen peroxide (H2O2), reduced and oxidized nicotinamide adenine dinucleotide phosphate (NADPH and NADP+), sodium dodecyl sulfate (SDS), sorbitol, tris(hydroxymethyl)aminomethane (Tris), pyrogallol, 2,4-dinitrophenylhydrazine and xylenol orange were from Sisco Research Laboratories, India. Thiobarbituric acid and trichloroacetic acid were from Himedia Laboratories (Mumbai, India).
All other chemicals used were of analytical grade.
All other chemicals used were of analytical grade.
6
Biochemical Assay Reagents and Materials
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L-thyroxine (T4), 6-propyl-2-thiouracil (PTU), CRM, Triton-X-100 and thiobarbituric acid were purchased from Sigma Chemical Co., USA. L–methionine, hydroxylamine hydrochloride, ethyelenediamine tetra acetic acid, riboflavin, phenol red, orthophosphoric acid, Sulfanilamide, N-(l-naphthyl) ethylenediaminedihydrochloride, hydrogen peroxide (H2O2), sodium hydroxide, ascorbic acid, ferric chloride, trichloroacetic acid, butylatedhydroxytoluene, metaphosphoric acid, and sodium dodecyl sulfate were obtained from SISCO Research Laboratories, Mumbai, India. All other chemicals otherwise not mentioned were of analytical grade.
7
Comprehensive Biochemical Protocols and Reagents
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Protein-A agarose, goat anti-rabbit IgG, 3, 3′, 5, 5′-tetramethylbenzidine (TMB), immobilon-P PVDF membrane, luminol, p-coumaric acid, Freund’s complete and incomplete adjuvants, testicular hyaluronidase, hyaluronic acid, o-dianisidine, horseradish peroxidase (HRP) 250U, and all chemicals were obtained from Sigma, St Louis, USA. Sodium chloride, bovine serum albumin, Folin-Ciocalteu reagent, Tris-HCl, casein, gelatin, trichloroacetic acid, sodium carbonate, calcium chloride, magnesium chloride, acrylamide and bis-acrylamide, sodium dodecyl sulfate (SDS), ammonium persulfate, tetramethyl ethylenediamine (TEMED), sodium formate, triton X-100, Alcian blue, acetic acid, triethanolamine, L-leucine, methanol, and all other chemicals were purchased from Sisco Research Laboratories (SRL), Mumbai, India. HRP-conjugated goat anti-horse IgG (H+L) was purchased from KINESISDx, South Paseo Dr, Brea, CA 90603, USA. Molecular weight markers were obtained from Genetix Biotech. Asia Pvt. Ltd. Bengaluru, India. LDH, CK, and CK-MB kits were purchased from AGAPPE Diagnostics Ltd. Kerala, India. Liquicelin-E and Uniplastin reagents were purchased from TULIP Diagnostics (P) Ltd. India.
8
Protocol for α-Synuclein Aggregation Assay
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The plasmid of α-synuclein was received as a kind gift from
Dr. Monica Sundd, National Institute of Immunology, New Delhi, India.
Tris buffer and copper sulfate pentahydrate were purchased from Merck.
ThT, DA hydrochloride, curcumin, and baicalein were purchased from
Sigma-Aldrich. Glycerol was purchased from SD Fine Chemicals, India,
whereas SDS was purchased from Sisco Research Laboratory, India. All
the solutions, except otherwise stated, were prepared in 20 mM Tris,
pH 7.5, and 0.1 M NaCl. Stock solutions of curcumin and baicalein
were prepared in 100% ethanol procured from Merck.
Dr. Monica Sundd, National Institute of Immunology, New Delhi, India.
Tris buffer and copper sulfate pentahydrate were purchased from Merck.
ThT, DA hydrochloride, curcumin, and baicalein were purchased from
Sigma-Aldrich. Glycerol was purchased from SD Fine Chemicals, India,
whereas SDS was purchased from Sisco Research Laboratory, India. All
the solutions, except otherwise stated, were prepared in 20 mM Tris,
pH 7.5, and 0.1 M NaCl. Stock solutions of curcumin and baicalein
were prepared in 100% ethanol procured from Merck.
9
Spectrophotometric Characterization of Bioactive Compounds
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BSA (fraction V), baicalein, TX-100, warfarin and ibuprofen were purchased from Sigma Co. (St. Louis, MO, USA). SDS, cetyltrimethylammonium bromide (CTAB) and other analytical grade reagents were acquired from Sisco Research Laboratory, India. The concentration of BSA was measured spectrophotometrically (Shimadzu UVPC 2450) by dissolving it in 20 mM phosphate buffer of pH 7.0 using a molar extinction coefficient of 43800 M−1 cm−1 at 280 nm [21] (link). The concentrations of baicalein, warfarin and ibuprofen were determined on a Shimadzu UVPC 2450 using their respective molar extinction coefficients: ε323 (baicalein) in ethanol=15700 M−1 cm−1, ε308 (warfarin)=12500 M−1 cm−1[22] (link), and ε222 (ibuprofen)=12700 M−1 cm−1[23] (link). All the experiments were performed in phosphate buffer containing less than 5% alcohol.
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