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Hct116 human colon adenocarcinoma cells

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HCT116 human colon adenocarcinoma cells are a well-characterized cell line derived from a human colon carcinoma. The cells exhibit an epithelial morphology and are commonly used in various research applications.

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2 protocols using hct116 human colon adenocarcinoma cells

1

Culturing HCT116 Colon Cancer Cells

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HCT116 human colon adenocarcinoma cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HCT116 was maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone Laboratories Inc., Logan, UT, USA) and antibiotics (100 µg/mL streptomycin and 100 U/mL penicillin) at 37 °C with 5% CO2.
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2

Culturing HCT116 Colon Adenocarcinoma Cells

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HCT116 human colon adenocarcinoma cells were purchased from the American Type Culture Collection (Manassas, VA, USA). HCT116 cells were maintained in DMEM supplemented with 10% heat-inactivated fetal bovine serum (FBS; HyClone Laboratories Inc., Logan, UT, USA) and antibiotics (100 μg/ml streptomycin and 100 U/ml penicillin) at 37 °C with 5% CO2.
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