V plex aβ peptide panel 1 4g8

CD36^−/− and WT mice were anesthetized with 1.5% isoflurane and placed on a stereotaxic device. A burr hole was drilled into the somatosensory cortex at coordinates: −1.58 mm anterior to bregma, 2.5 mm lateral, and 0.4 mm from the dura. Human Aβ₁₋₄₀ (100 mmol/L; rPeptide, Watkinsville, GA) was slowly injected in a volume of 1 µl using an Ultramicropump (World Precision Instruments, Sarasota, FL). One hour later blood samples were collected from the superior sagittal sinus (SSS) and heart, and the site of neocortical injection was sampled. Samples were stored at −80 °C until assay. Plasma and brain Aβ₁₋₄₀ levels were quantified using V-PLEX Aβ Peptide Panel 1 (4G8) [Stock # K15199E-2, Meso Scale Discovery (MSD), Rockville, MD, USA] according to the manufacturer instructions.

CD36^−/− and WT mice were anesthetized with 1.5% isoflurane and placed on a stereotaxic device. A burr hole was drilled into the somatosensory cortex at coordinates: -1.58 mm anterior to bregma, 2.5 mm lateral, and 0.4 mm from the dura. Human Aβ_1-40 (100 mmol/L; rPeptide, Watkinsville, GA) was slowly injected in a volume of 1 µl using an Ultramicropump (World Precision Instruments, Sarasota, FL). One hour later blood samples were collected from the superior sagittal sinus (SSS) and heart, and the site of neocortical injection was sampled. Samples were stored at -80 °C until assay. Plasma and brain Aβ_1-40 levels were quantified using V-PLEX Aβ Peptide Panel 1 (4G8) [Stock # K15199E-2, Meso Scale Discovery (MSD), Rockville, MD, USA] according to the manufacturer instructions.