Respiratory function was evaluated every 6 hours throughout 24 hours of xenogeneic XC. Blood samples were collected from the PA and PV, blood gas analysis was performed using a point-of-care analyzer (epoc, Heska), and gas exchange parameters (PaO2/FiO2, change in pCO2) were calculated. Peak inspiratory pressure (PIP) and plateau pressure (Pplateau) were recorded to determine dynamic compliance (Cdyn = TV/(PIP − PEEP)) of ex vivo human donor lungs. Vascular flows and pressures at the PA and PV were continuously monitored and recorded (ADInstruments). Lung weight was obtained using a top-loading balance (W-4830, WeighMax). Airways were assessed with a 3.8 mm flexible bronchoscope (aScope 4, Ambu).
Ascope 4
Manufactured by Ambu
Sourced in Denmark
The AScope 4 is a lightweight, portable video laryngoscope designed for intubation procedures. It features a high-resolution camera and a bright LED light source to provide clear visual access to the patient's airway during intubation. The AScope 4 is intended for use by trained medical professionals.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ascope 4
1
Xenogeneic Lung Perfusion Assessment
2
Bronchoalveolar Lavage for COVID-19 Diagnosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All bronchoscopies were performed at both centers for diagnostic purposes in order to investigate the presence of other co-infections and no complications related to bronchoscopy occurred in patients [9 (link)].
BAL was collected from mechanically ventilated ICU patients according to the clinical requirement through disposable bronchoscope aScope™ 4 (Ambu A/S, Baltorpbakken, Denmark) according to WHO guidelines. Security procedures were strictly observed, and only essential personnel were involved in endoscopic exams. The Personal Protective Equipment included water-resistant gowns, gloves, respiratory protection (FFP3 mask), and eye protection. Six patients underwent repeated bronchoscopies. Therefore, the number of overall BAL samples were 45. When disposable bronchoscopes were not available, we followed the standard High-Level Disinfection for the utilization of re-usable bronchoscopes. BAL specimens were managed in a biosafety level 3 laboratory until inactivation. BALs were centrifuged at 400 g for 10 min at room temperature, inactivated with a 0.2% SDS and 0.1% Tween-20 solution followed by 65 °C for 15 min. BAL supernatants were then stored at − 20 °C until analysis. Cell pellets were fixed, then stained with Papanicolaou to analyze differential cell count or used for ultrastructural analysis.
BAL was collected from mechanically ventilated ICU patients according to the clinical requirement through disposable bronchoscope aScope™ 4 (Ambu A/S, Baltorpbakken, Denmark) according to WHO guidelines. Security procedures were strictly observed, and only essential personnel were involved in endoscopic exams. The Personal Protective Equipment included water-resistant gowns, gloves, respiratory protection (FFP3 mask), and eye protection. Six patients underwent repeated bronchoscopies. Therefore, the number of overall BAL samples were 45. When disposable bronchoscopes were not available, we followed the standard High-Level Disinfection for the utilization of re-usable bronchoscopes. BAL specimens were managed in a biosafety level 3 laboratory until inactivation. BALs were centrifuged at 400 g for 10 min at room temperature, inactivated with a 0.2% SDS and 0.1% Tween-20 solution followed by 65 °C for 15 min. BAL supernatants were then stored at − 20 °C until analysis. Cell pellets were fixed, then stained with Papanicolaou to analyze differential cell count or used for ultrastructural analysis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!