Lcms 20ad

¹H NMR spectra were recorded on a Bruker ARX 400. HPLC was carried out at a LUMTECH HPLC (Germany) system using a C₁₈ RP column with MeOH (0.1% of TFA) and water (0.1% of TFA) as the eluents. LC-MS was conducted at the LCMS-20AD (Shimadzu) system. High resolution mass spectra (HR-MS) were received from VG ZAB-HS system (England). UV-vis spectra were measured on a Shimadzu UV-1700 spectrometer. Photoluminescence spectra were measured on a Perkin-Elmer LS 55 spectrofluorometer. Cellular imaging was performed by confocal laser scanning microscopy (CLSM, Zeiss LSM 410, Jena, Germany). Non-invasive in vivo fluorescence imaging was conducted on a Maestro EX system (CRi, Inc.).

¹H NMR spectra were recorded on a Bruker ARX 400 using DMSO-d₆ as the solvent. High-pressure liquid chromatography (HPLC) was conducted at a LUMTECH HPLC (Germany) system using a C₁₈ RP column with MeOH (0.05% of TFA) and water (0.05% of TFA) as the eluents. Liquid chromatography mass spectra (LC-MS) were performed at a LCMS-20AD (Shimadzu) system. UV-vis spectra were measured on a Shimadzu UV-1700 spectrometer. Photoluminescence spectra were measured on a Perkin-Elmer LS 55 spectrofluorometer. The sample morphology was studied by transmission electron microscopy (JEM-2010F, JEOL, Japan). Rheology test was carried out on an AR 1500ex (TA instrument) system, and 40 mm parallel plates was used during the experiment at the gap of 500 μm. For the dynamic frequency sweep, the solution of Rhodamine-GFFYE-CS-EE was directly transferred to the rheometer in the region of 0.1–100 rad/s at the strain of 1%.

The synthesized compounds were characterized using ¹H NMR (Bruker ARX 400). LC-MS spectrometric analyses were performed at the LCMS-20AD (Shimadzu) system. HPLC was conducted at LUMTECH HPLC (Germany) system using a C18 RP column with MeOH (0.05% of TFA) and water (0.05% of TFA) as the eluents. Rheology was performed on an AR 2000ex (TA instrument) system using a parallel plates (40 mm) at the gap of 500 μm. MTT data was recorded on a BioTek Synergy™ 4 Hybrid Microplate Reader. TEM images were done on a Tecnai G2 F20 system, operating at 200 kV. The zeta potentials of the self-assembled nanofibers were measured by a zeta potential analyzer (Zeta Pals, Brookhaven Instruments, Huntsville, NY, USA). Before measurements, the hydrogels formed by adding different equiv. of doxorubicin to the 0.5 wt% peptide solution and peptide solution itself were all diluted for 5 times by 1 × PBS, affording corresponding nanofiber solutions. The zeta potential was then measured with palladium electrodes at 25°C, and the mean value of three readings was taken.