Luciferase kits

Manufactured by Promega

Sourced in United States

Promega luciferase kits are a series of bioluminescent assay reagents designed to measure the activity of the luciferase enzyme. These kits provide a quantitative assessment of luciferase expression levels, which can be used as a reporter for gene expression or other biological processes.

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Lab products found in correlation

24 well plate, by Corning (1 mentions) 12 well cell culture plate, by Corning (1 mentions)

Close spelling variants of this product

Dual-Luciferases Reporter Assay kit Promega Luciferase Reporter detection kits Dual-Glo Luciferase Reporter kit Dual-luciferase reporter gene uorescence detection kit Luciferase Assay Substrate Kit Passive Lysis Buffer of the Dual Luciferase Reporter Assay Kit Luciferase activity detection kit Nano-Glo Luciferase Assay Reagent Kit CellTiter-Glo luciferase kit Luciferase test kit

5 protocols using luciferase kits

Luciferase Assay for Smad4 Binding

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The luciferase kits were obtained from Promega Corporation, Fitchburg, WI, USA. The experiments were performed following the protocol from the manufacturer. The luciferase reporter of Smad4 binding site (SBE-Luc) was from Dr Fan Feng in Center of Therapeutic Research for Hepatocellular Carcinoma, Beijing 302nd Hospital, Beijing, People’s Republic of China.18 (link) Briefly, A549 or H460 cells were seeded in 24-well plates (Corning Incorporated) containing RPMI 1640 medium supplemented with 10% fetal bovine serum. Cells were co-transfected with the luciferase reporters and then harvested after culture for 24 hours. The luciferase and β-galactosidase activities were then examined. The luciferase assays were performed three times with similar results.

Qin H., Sha J., Jiang C., Gao X., Qu L., Yan H., Xu T., Jiang Q, & Gao H. (2015). miR-122 inhibits metastasis and epithelial–mesenchymal transition of non-small-cell lung cancer cells. OncoTargets and therapy, 8, 3175-3184.

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HepG2/STAT3 Luciferase Assay Protocol

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The HepG2/STAT3 luciferase reporter cells (3.0*10⁵ per well) were seeded into 12 well cell culture plates (Corning) and allowed to grow for 24 h and then treated with EB for 2 h followed by stimulation with IL-6 (50 ng/mL) for 5 h. Luciferase activity was determined using the Promega luciferase kits according to the manufacturer’s instruction. The cell number was counted at seeding and equal number of cells was seeded. All luciferase assay experiments were performed in triplicates to minimize the difference caused by cell number.

Yu X., He L., Cao P, & Yu Q. (2015). Eriocalyxin B Inhibits STAT3 Signaling by Covalently Targeting STAT3 and Blocking Phosphorylation and Activation of STAT3. PLoS ONE, 10(5), e0128406.

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NF-κB Activation Assay in HEK293 Cells

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HEK293/NF-κB cells were seeded into 96-well cell culture plates and allowed to grow for 48 h and cells were then treated with 2-MS for 2 h followed by stimulation with 2 ng/mL TNF-α for 5 h. Luciferase activity was determined using the Promega luciferase kits according to the manufacturer's instruction (Promega, Madison, WI, USA). All luciferase assay experiments were repeated at least twice.

Kuang S., Qi C., Liu J., Sun X., Zhang Q., Sima Z., Liu J., Li W, & Yu Q. (2014). 2-Methoxystypandrone inhibits signal transducer and activator of transcription 3 and nuclear factor-κB signaling by inhibiting Janus kinase 2 and IκB kinase. Cancer Science, 105(4), 473-480.

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Activation of STAT3 in HepG2 Cells

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HepG2/STAT3 cells were seeded onto 96-well cell culture plates and grew to 90% confluence. Cells were then treated with PN for 1 h followed by stimulation with 10 ng/mL IL-6 for 4 h. Luciferase activity was determined using Promega luciferase kits according to the manufacturer’s instruction (Promega, Madison, WI, USA).

Liu M., Xiao C., Sun M., Tan M., Hu L, & Yu Q. (2018). Parthenolide Inhibits STAT3 Signaling by Covalently Targeting Janus Kinases. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1478.

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Xanthatin Modulates NF-κB and STAT3 Signaling

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HEK293/NF‐κB or HepG2/STAT3 cells were seeded into 96‐well cell culture plates and cultured to 90% confluence. Cells were then treated with xanthatin for 1 hour followed by stimulation with 1 ng/mL TNFα or 10 ng/mL IL‐6 for 4 hours. Luciferase activities were determined using the Promega luciferase kits according to the manufacturer's instructions (Promega, Madison, WI, USA).

Liu M., Xiao C., Sun M., Tan M., Hu L, & Yu Q. (2019). Xanthatin inhibits STAT3 and NF‐κB signalling by covalently binding to JAK and IKK kinases. Journal of Cellular and Molecular Medicine, 23(6), 4301-4312.

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