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1 1 1 3 3 3 hexafluoroisopropanol

Manufactured by Merck Group
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Sourced in Germany

1,1,1,3,3,3-hexafluoroisopropanol is a specialty chemical compound used as a solvent and reagent in various laboratory applications. It has a high polarity and a low boiling point, making it useful for tasks that require a volatile, aprotic solvent.

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Lab products found in correlation

Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine dopc, by Avanti Polar Lipids (1 mentions) Methanol, by Merck Group (1 mentions) Dichloromethane, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Gelatin, by Merck Group (1 mentions) Urea nh2 2co, by Merck Group (1 mentions) Trypsin ethylenediaminetetraacetic acid, by Thermo Fisher Scientific (1 mentions) Polycaprolactone, by Merck Group (1 mentions) Alizarin red s, by Merck Group (1 mentions) Nutrient mixture f 12, by Thermo Fisher Scientific (1 mentions) Hyaluronic acid, by Merck Group (1 mentions) Celltiter 96 aqueous one solution, by Promega (1 mentions) Trypan blue, by Thermo Fisher Scientific (1 mentions) Curcumin, by Merck Group (1 mentions) α tocopherol, by Merck Group (1 mentions) Human dermal fibroblasts, by American Type Culture Collection (1 mentions) Antibiotic antimycotic, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions)

Close spelling variants of this product

Hexafluoroisopropanol (45 citations) 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) (12 citations) 3-hexafluoroisopropanol (2 citations)

5 protocols using 1 1 1 3 3 3 hexafluoroisopropanol

1

Analysis of Lipid Membrane Composition

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Dichloromethane and methanol, HPLC-grade solvents, were purchased from Merck (Darmstadt, Germany), while 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) was supplied by Sigma-Aldrich (St. Louis, MO, USA). 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-didocosahexaenoyl-sn-glycero-3 phosphocholine (22∶6(cis)PC) were obtained from Avanti Polar Lipids (Birmingham, AL, USA).
Sublimi Saponetti M., Grimaldi M., Scrima M., Albonetti C., Nori S.L., Cucolo A., Bobba F, & D'Ursi A.M. (2014). Aggregation of Aß(25-35) on DOPC and DOPC/DHA Bilayers: An Atomic Force Microscopy Study. PLoS ONE, 9(12), e115780.
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2

Synthesis of PHB-Gelatin Composite

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Ferric (III) chloride hexahydrate (FeCl3-6H2O, Sigma-Aldrich, Steinheim, Germany), ferric (II) sulfate heptahydrate (FeSO4-7H2O, Sigma-Aldrich, Steinheim, Germany), 1,1,1,3,3,3-hexafluoroisopropanol (HFIP, Saint Louis, MO, USA), urea ((NH2)2CO, Sigma-Aldrich, Steinheim, Germany), Poly(3-hydroxybutyrate) (PHB, natural origin, MW 500,000) and gelatin (from porcine skin, type A) were purchased from Merck (Sigma-Aldrich, Steinheim, Germany). Deionized water obtained by Millipore Milli-Q system (Darmstadt, Germany) was used in all experiments.
Pryadko A.S., Botvin V.V., Mukhortova Y.R., Pariy I., Wagner D.V., Laktionov P.P., Chernonosova V.S., Chelobanov B.P., Chernozem R.V., Surmeneva M.A., Kholkin A.L, & Surmenev R.A. (2022). Core-Shell Magnetoactive PHB/Gelatin/Magnetite Composite Electrospun Scaffolds for Biomedical Applications. Polymers, 14(3), 529.
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3

Biomimetic Scaffold for Tissue Engineering

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Polycaprolactone (PCL), hyaluronic acid, (HA) minocycline hydrochloride (MH), 1,1,1,3,3,3-hexafluoro-isopropanol (HFIP), methanol, Alizarin red-S, cetylpyridinium chloride were purchased from Sigma-Aldrich. Silk fibroin (SF) was purchased from Zhang Peng International Trading, Singapore. Dulbecco’s modified eagle’s medium (DMEM), nutrient mixture F-12, fetal bovine serum (FBS), antibiotics and trypsin-ethylene diamine tetra acetic acid (EDTA) were procured from GIBCO (Invitrogen, Carlsbad, CA, USA). CellTiter 96® Aqueous one solution was obtained from Promega, Madison, WI, USA.
Tham A.Y., Gandhimathi C., Praveena J., Venugopal J.R., Ramakrishna S, & Kumar S.D. (2016). Minocycline Loaded Hybrid Composites Nanoparticles for Mesenchymal Stem Cells Differentiation into Osteogenesis. International Journal of Molecular Sciences, 17(8), 1222.
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4

Fibroblast-based Tissue Engineering Protocol

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Human dermal fibroblasts were obtained from the American Type Culture Collection (Manassas, VA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM), antimycotic antibiotics, fetal bovine serum (FBS), trypan blue, and trypsin–ethylenediaminetetraacetate (EDTA) were obtained from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). PLACL (70:30, Mw 150 kDa) was purchased from Boehringer Ingelheim Pharma, GmbH & Co., Ingelheim, Germany. SF was obtained from Zhang Peng International Trading, Singapore. Curcumin, α-tocopherol and 1,1,1,3,3,3-hexafluoro-isopropanol (HFIP) were obtained from Sigma-Aldrich, (St Louis, MO, USA).
Gandhimathi C., Venugopal J.R., Bhaarathy V., Ramakrishna S, & Kumar S.D. (2014). Biocomposite nanofibrous strategies for the controlled release of biomolecules for skin tissue regeneration. International Journal of Nanomedicine, 9, 4709-4722.
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5

Gelatin-based Biomaterial Characterization

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Gelatin (powder, purity > 99.5%) was supplied by the Sinopharm Chemical Reagent Co., Ltd), while dimethyl sulfoxide (DMSO) was sourced from J&K Chemicals. Dulbecco's Modified Eagle Medium (DMEM) was provided from Jinuo Biological Medicine Technology Ltd. Poly(lactide-co-ε-caprolactone) (PLCL, PCL:PLLA=1:1) came from Jinan Daigang Biomaterials. 1,1,1,3,3,3-Hexafluoroisopropanol (HFIP, purity > 95.5%), ciprofloxacin (purity > 98%), phosphate-buffered saline (PBS, sodium phosphate=10 mM, NaCl=150 mM; pH=7.4), sodium bicarbonate (SB, powder, purity > 99.5%), penicillin/streptomycin, thiazolyl blue tetrazolium bromide (MTT) were supplied by Sigma-Aldrich Ltd. L929 cells were purchased from the Chinese Institute of Biochemistry and Cell Biology. An acetic acid buffer (AA, sodium acetate=25 mM; pH=5.0) was prepared in-house. All other chemicals were at least analytical grade, and water was doubly distilled prior to use.
Sang Q., Williams G.R., Wu H., Liu K., Li H, & Zhu L.M. (2017). Electrospun gelatin/sodium bicarbonate and poly(lactide-co-ε-caprolactone)/sodium bicarbonate nanofibers as drug delivery systems. Materials science & engineering. C, Materials for biological applications, 81.
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