Triple quadruple mass spectrometer

Manufactured by Waters Corporation

The Triple quadruple mass spectrometer is an analytical instrument used for the identification and quantification of chemical compounds. It combines two quadrupole mass analyzers with a collision cell, enabling the detection and analysis of complex molecular structures.

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Lab products found in correlation

Acquity uplc sample manager, by Waters Corporation (2 mentions) Positive ion electrospray ionization, by Waters Corporation (2 mentions) Acquity tqd, by Waters Corporation (1 mentions) Agilent infinity 1100, by Agilent Technologies (1 mentions) Quaternary pump, by Agilent Technologies (1 mentions) H class uplc, by Waters Corporation (1 mentions) Gemini c18 column, by Phenomenex (1 mentions) Lc 20a, by Shimadzu (1 mentions) Masslynx software, by Waters Corporation (1 mentions) Spd m20a, by Shimadzu (1 mentions) Lc solution software, by Shimadzu (1 mentions) Lc 20at, by Shimadzu (1 mentions) Dgu 20a3, by Shimadzu (1 mentions) Cto 20a, by Shimadzu (1 mentions) Sil 20ac, by Shimadzu (1 mentions)

Spelling variants of this product

XEVO TQD triple quadruple mass spectrometer (8 citations) Xevo TQ-S triple quadruple mass spectrometer (4 citations) Xevo-TQ triple quadruple mass spectrometer (3 citations) XEVO TQD triple quadruple instrument and mass spectrometer (2 citations)

4 protocols using triple quadruple mass spectrometer

Sensitive UPLC-MS/MS Quantitation of Fentanyl

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Fentanyl in plasma was quantitated using a validated UPLC-MS/MS method consisting of a Waters Acquity UPLC sample manager coupled to a triple quadruple mass spectrometer operating in the multiple reaction monitoring mode (MRM) with positive ion electrospray ionization (Waters, Etten-Leur, The Netherlands). The multiple reaction monitoring transition was set at 337→188.
Chromatographic separation of fentanyl was achieved on an Acquity UPLC^® BEH C18 1.7 μm 2.1 x 100 mm column eluted at a flow-rate of 0.350 mL/min on a gradient of methanol. The overall cycle time of the method was 6 minutes. The calibration curves were linear over the range of 0.1 to 10 ng/mL with the lower limit of quantitation validated at 0.1 ng/mL for fentanyl. The within and between-run precisions at five tested concentrations, including the LLQ, were ≤ 5.5% and ≤ 6.1%, respectively, while the average accuracy ranged from 86.2% to 97.5%. The extraction of 200 μL of plasma involved a deproteinization step with acetone followed by a simple liquid extraction with ethyl acetate. The organic phase was evaporated and subsequently dissolved in 100 μL methanolic solutions, from which aliquots of 10 μL were injected into the UPLC-MS/MS system.

Kuip E.J., Oldenmenger W.H., Thijs—Visser M.F., de Bruijn P., Oosten A.W., Oomen—de Hoop E., Koolen S.L., Van der Rijt C.C, & Mathijssen R.H. (2018). Effects of smoking and body mass index on the exposure of fentanyl in patients with cancer. PLoS ONE, 13(6), e0198289.

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HPLC and LC/MS/MS Analysis of PD98059

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An Agilent HPLC workstation was used for sample analysis (Agilent Infinity 1100, Santa Clara, CA) that consisted of an Agilent quaternary pump, automatic injection port, and Agilent diode array detector (Agilent Corporation, Santa Clara, CA). A RP-C18 column was used for analysis (Waters Symmetry, 5 μm pore size, 4.6 mm × 150 mm, Milford, MA). The mobile phase consisted of methanol: water 70:30 with 0.1% v/v trifluoroacetic acid, and the flow rate was 1 ml/min at room temperature. The detection wavelength was set to 275 nm.
The LC/MS/MS system consisted of a Waters Acquity TQD (Milliford, MA), which includes a triple quadruple mass spectrometer and Acquity H-Class UPLC. The same column, temperature, mobile phase, and flow rate stated above with the HPLC method were used. Quantitative analysis of PD98059 and IS was carried out using positive electrospray ionization via the highly sensitive and specific MRM mode. PD98059 was detected at 3 transition channels for brain samples (268.03→104.86, 268.03→121.01, and 268.03→133.06) and 5 transition channels for plasma samples (268.03→104.86, 268.03→121.01, 268.03→133.06, 268.03→148.08, and 268.03→236.07), while the IS was detected as 3 transition channels in both brain and plasma samples (239.03→77.04, 239.03→129.03, 239.03→136.97). The standard curves were linear over a range of 0.1–30 μg/ml for both plasma and brain.

Naguib Y.W., Givens B.E., Ho G., Yu Y., Wei S.G., Weiss R.M., Felder R.B, & Salem A.K. (2021). An injectable microparticle formulation for the sustained release of the specific MEK inhibitor PD98059: in vitro evaluation and pharmacokinetics. Drug delivery and translational research, 11(1), 182-191.

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Fentanyl Quantification in EDTA Plasma

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We quantified fentanyl in EDTA plasma. A validated UPLC-MS/MS method. This method consisted of a Waters Acquity UPLC sample manager, coupled to a triple quadruple mass spectrometer operating in the multiple reaction monitoring mode (MRM) with positive ion electro spray ionization (Waters, Etten-Leur, The Netherlands). The multiple reaction monitoring transitions was set at 337→188.
Chromatographic separations for fentanyl were achieved on an Acquity UPLC^® BEH C18 1.7 μm 2.1 × 100 mm column eluted at a flow-rate of 0.350 mL/min on a gradient of methanol. A cycle time for this method was about 6 minutes. Calibration curves were linear over a wide range (0.100 to 10.0 ng/mL) with at lower limit of quantitation (LLQ) of 0.100 ng/mL for fentanyl. The within and between-run precisions, including the LLQ, were ≤ 5.52 % and ≤ 6.12 %, respectively, while the average accuracy ranged from 86.2 % to 97.5%. The extraction of 200 μL of plasma involved a deproteinization step with acetone, followed by a simple liquid extraction with ethyl acetate. The organic phase was evaporated and subsequently dissolved in 100 μL methanolic solutions, from which aliquots of 10 μL were injected into the UPLC-MS/MS system.

Kuip E.J., Oldenmenger W.H., Visser-Thijs M.F., de Bruijn P., Oomen-de Hoop E., Mathijssen R.H., Van der Rijt C.C, & Koolen S.W. (2018). Influence of aprepitant and localization of the patch on fentanyl exposure in patients with cancer using transdermal fentanyl. Oncotarget, 9(26), 18269-18276.

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HPLC-MS Analysis of Analytes

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The HPLC analysis was performed using a Shimadzu LC-20A (Shimadzu Co., Kyoto, Japan), which consisted of a pump (LC-20AT), on-line degasser (DGU-20A₃), column oven (CTO-20A), autosampler (SIL-20 AC), and PDA detector (SPD-M20A). The data were processed with LCsolution software (Version 1.24, Shimadzu, Kyoto, Japan). The analytes were separated on a Phenomenex Gemini C₁₈ column (250×4.6 mm, 5 μm, Torrance, CA, USA) maintained at 40°C. The gradient elution of mobile phases A (1.0% v/v aqueous acetic acid) and B (acetonitrile with 1.0% v/v acetic acid) was conducted as follows: 15-20% B for 0–10 min, 20-70% B for 10–30 min, 70-100% B for 30–40 min, 100% B for 40–45 min, and 100-15% B for 45–50 min. The flow rate was 1.0 mL/min and injection volume was 10 μL. The PDA detector was monitored at 203 nm, 254 nm, and 280 nm.
The mass spectrometer was operated using a Waters triple quadruple mass spectrometer equipped with electrospray ionization (ESI) source. The MS conditions were as follows: capillary voltage, 3.3 kV; extractor voltage, 3 V; RF lens voltage, 0.3 V; source temperature, 120°C; desolvation temperature, 300°C; desolvation gas, 600 L/h; cone gas, 50 L/h; collision gas, 0.14 mL/min. Data acquisition was processed by Waters MassLynx software (version 4.1, Milford, MA, USA).

Seo C.S., Kim O.S., Kim Y, & Shin H.K. (2014). Simultaneous quantification and inhibitory effect on LDL oxidation of the traditional Korean medicine, Leejung-tang. BMC Complementary and Alternative Medicine, 14, 3.

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