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Hrp conjugated total h3

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HRP-conjugated Total H3 is a laboratory reagent used to detect and quantify the total histone H3 protein in biological samples. It consists of an anti-histone H3 antibody conjugated to horseradish peroxidase (HRP), which can be used in various immunoassay techniques such as Western blotting and ELISA.

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Lab products found in correlation

Bca protein assay, by Thermo Fisher Scientific (4 mentions) Ab4469, by Abcam (2 mentions) Suz12, by Cell Signaling Technology (2 mentions) Hrp conjugated actin, by Abcam (2 mentions) Nupage novex 4 12 bis tris protein gel, by Thermo Fisher Scientific (2 mentions) 0.45 μm nitrocellulose membrane, by Bio-Rad (2 mentions) Halt protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) H3k27me3, by Cell Signaling Technology (2 mentions) Ripa buffer, by Cell Signaling Technology (2 mentions) H3k27ac, by Abcam (2 mentions)

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Total H3 (19 citations)

2 protocols using hrp conjugated total h3

1

Western Blot Analysis of Chromatin Regulators

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Cell lysates were prepared in RIPA buffer (#9806, Cell Signaling) supplemented with Halt protease and phosphatase inhibitor cocktail (#78440, Thermo Scientific). Equal amounts of protein, as measured by BCA protein assay (#23225, Thermo Scientific), were resolved in NuPAGE® Novex® 4–12% Bis-Tris Protein Gels (#NP0321BOX, Life Technologies) and transferred electrophoretically onto a Nitrocellulose 0.45 μm membrane (#162-0115, BioRad). Membranes were blocked for 1 hour at room temperature in 5% BSA in TBST before being incubated overnight at 4°C with the primary antibodies diluted at 1:1000 in 5% BSA in TBST. NF1 (#sc-67, Santa Cruz, 1:500), EZH1 (#ab13665, Abcam, 1:1000), EZH2 (#5246, Cell signaling,1:1000), SUZ12 (#3737, Cell Signaling, 1:1000), EED (#ab4469, Abcam, 1:1000), H3K27me3 (#9733, Cell Signaling, 1:1000), H3K27Ac (#ab4729, Abcam, 1:1000), HRP-conjugated Total H3 (#12648, Cell Signaling, 1:1000), and HRP-conjugated Actin(#ab49900, Abcam, 1:5000) antibodies were used.
Lee W., Teckie S., Wiesner T., Ran L., Prieto Granada C.N., Lin M., Zhu S., Cao Z., Liang Y., Sboner A., Tap W.D., Fletcher J.A., Huberman K.H., Qin L.X., Viale A., Singer S., Zheng D., Berger M.F., Chen Y., Antonescu C.R, & Chi P. (2014). PRC2 is recurrently inactivated through EED or SUZ12 loss in malignant peripheral nerve sheath tumors. Nature genetics, 46(11), 1227-1232.
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2

Western Blot Analysis of Chromatin Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were prepared in RIPA buffer (#9806, Cell Signaling) supplemented with Halt protease and phosphatase inhibitor cocktail (#78440, Thermo Scientific). Equal amounts of protein, as measured by BCA protein assay (#23225, Thermo Scientific), were resolved in NuPAGE® Novex® 4–12% Bis-Tris Protein Gels (#NP0321BOX, Life Technologies) and transferred electrophoretically onto a Nitrocellulose 0.45 μm membrane (#162-0115, BioRad). Membranes were blocked for 1 hour at room temperature in 5% BSA in TBST before being incubated overnight at 4°C with the primary antibodies diluted at 1:1000 in 5% BSA in TBST. NF1 (#sc-67, Santa Cruz, 1:500), EZH1 (#ab13665, Abcam, 1:1000), EZH2 (#5246, Cell signaling,1:1000), SUZ12 (#3737, Cell Signaling, 1:1000), EED (#ab4469, Abcam, 1:1000), H3K27me3 (#9733, Cell Signaling, 1:1000), H3K27Ac (#ab4729, Abcam, 1:1000), HRP-conjugated Total H3 (#12648, Cell Signaling, 1:1000), and HRP-conjugated Actin(#ab49900, Abcam, 1:5000) antibodies were used.
Lee W., Teckie S., Wiesner T., Ran L., Prieto Granada C.N., Lin M., Zhu S., Cao Z., Liang Y., Sboner A., Tap W.D., Fletcher J.A., Huberman K.H., Qin L.X., Viale A., Singer S., Zheng D., Berger M.F., Chen Y., Antonescu C.R, & Chi P. (2014). PRC2 is recurrently inactivated through EED or SUZ12 loss in malignant peripheral nerve sheath tumors. Nature genetics, 46(11), 1227-1232.
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