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Dxh 800 hematology analyzer

Manufactured by Beckman Coulter
Sourced in United States

The DxH 800 is a hematology analyzer designed for routine blood analysis in clinical laboratories. It provides automated counting and differentiation of blood cells, including red blood cells, white blood cells, and platelets. The DxH 800 utilizes advanced technology to deliver reliable and accurate results, supporting healthcare professionals in their diagnostic and treatment decisions.

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17 protocols using dxh 800 hematology analyzer

1

Comprehensive Clinical Biochemistry Analysis

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Cobas C-311m, a fully automated and closed clinical chemistry analyzer (Roche/Hitachi Cobas C-311, Roche diagnostics GmbH, Mannheim, Germany) was used to perform LFTs including aspartate transaminase (AST), alanine transaminase (ALT), Alkaline phosphatase (ALP), serum bilirubin (total and direct), albumin, and serum creatinine tests. DxH 800 hematology analyzer (Beckman Coulter, Germany) was used to conduct platelet count. Moreover, advanced quality one-step HBsAg test kit (InTec PRODUCTS, INC.) and a one-step HCV test kit (Guangzhou Wondfo Biotech Co. Ltd) were used for HBsAg and anti-HCV screening, respectively.
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2

Sepsis Biomarkers Monitoring Protocol

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Fasting peripheral blood (3mL) was drawn from the patients at the time of admission and placed in vacuum tubes without anticoagulant and anticoagulant tubes containing Ethylenediamine tetraacetic acid-K2 (EDTA-K2). Next, the blood was centrifuged for 10min with a radius of 10cm and a centrifugal force of 1450(×g) to separate the serum. The CRP and PCT (Chundu Biotechnology Co., Ltd., Wuhan, China, batch no: CDJ-1296C-SJH, CD-0208-LIN) in the serum were detected with Johnson Vitros5600 automatic biochemical-immune analyzer (Johnson & Johnson clinical diagnostics, Inc., NY, USA). The operations were carried out in strict accordance with the instruction manual of the instrument and kit. DxH 800 hematology analyzer (Beckman Coulter, Chaska, MN, USA) was used to measure N% in peripheral blood. These indexes of patients in the SAP group were repeatedly detected on the 3rd, 5th and 7th day of admission. Normal CRP: 0–8 mg/L (18 (link)); normal PCT: 0–0.05 ng/ml (19 (link)); Normal N%: 60–75% (20 (link)).
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3

Peripheral Blood Cell Ratio Analysis

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NLR and PLR were defined as the absolute neutrophil count divided by the absolute lymphocyte count, and the absolute platelet count divided by the absolute lymphocyte count, respectively; MLR was the result of the ratio between the absolute monocyte count and absolute lymphocyte count. These parameters were analyzed from peripheral blood cell count by DxH 800 hematology analyzer (Beckman Coulter). Full blood count data were eligible for analysis if performed within 2 months before the start of first line and 3 days for second-line.
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4

Validating Leukocyte Isolation Protocol

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Two reference systems were used to verify the techniques used in this study. First, a Coulter counter (DxH 800 Hematology Analyzer, Beckman Coulter, Indianapolis, IN, USA) was used to obtain the reference CBC data for the blood samples used in the analysis. Personal identity data such as names and addresses are protected by law and information regarding the individuals’ health and financial circumstances are protected. This CBC result was used as comparative data to verify the accuracy of the parameters developed for shadow image analysis. Flow cytometry (Guava easyCyte 8-10HT, Millipore, Burlington, MA, USA) was used to determine the purity of the isolated leukocyte samples. Since flow cytometry can distinguish types of blood cells using fluorescent antibodies, it is possible to confirm the purity of the separated leukocytes. The results of this analysis confirmed that high purity and high concentration leukocyte samples were obtained.
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5

Pediatric Venous Blood Collection and Analysis

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Non-fasting blood samples of 10 mL peripheral venous blood were obtained from each child by a trained pediatrician. Blood was drawn into plain and EDTA-containing VACUETTE® Blood Collection Tubes (Greiner Bio one GmbH, Kremsmünster, Austria) handled, and stored by trained staff according to accepted protocols. The blood samples were transferred in cool conditions to a central clinical diagnostic laboratory at Hillel Yaffe Medical Center within 2 h of collection. All assays were performed on the same day in a blinded manner to the demographic, clinical, and nutritional data. A complete blood count (CBC) was conducted using a DxH-800 hematology analyzer (Beckman Coulter Inc., Brea, CA, USA). Levels of biochemical markers in serum were measured using Cobas-8000 instrument with Roche Diagnostics kits according to the manufacturer’s procedures (Roche Diagnostics GmbH, Mannheim, Germany).
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6

Biomarker Profiling of Patient Serum

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Before treatment, we collected 3ml of peripheral venous blood from all patients. Serum CEA, CA125, and CA153 were measured using an automatic chemiluminescence immunoassay system (SIEMENS ADVIA centaur; Siemens, Germany). NLR was the absolute neutrophil count/the total lymphocyte, LMR was the total lymphocyte/the absolute monocyte, and PLR was the total platelet count/the total lymphocyte count. These parameters were analyzed from the peripheral blood cell count (DxH 800 hematology analyzer, Beckman Coulter). See Supplementary for the experimental methods of cellular immunology related indicators.
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7

Comprehensive Blood Profile Analysis

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Peripheral venous blood samples were sampled via the antecubital vein in the morning after an overnight fasting. Complete blood counts and differential counts were analyzed by the automated cellular analysis system Beckman Coulter DxH 800 hematology analyzer (Beckman Coulter, Miami, FL). Biochemical parameters of albumin, lipid profile, high sensitive-C reactive protein (hs-CRP), fasting glucose, aspartate transaminase, blood urea nitrogen, and serum creatinine were measured by the ARCHITECT i2000SR immunoassay analyzer (Abbott Diagnostics, Abbott Park, IL, USA). Lymphopenia was defined by absolute lymphocyte count < 1000/cumm [29 (link)].
Lymphocyte subsets were determined flow-cytometric analysis system Beckman Coulter FC500 flow cytometer (Beckman Coulter, Miami, FL) in freshly drawn peripheral blood after adequate processing as the manufacturer’s instruction. Serum CMV IgG, VZV IgG, HBsAg IgG, HCV IgG, and C. pneumoniae IgG were measured by a chemiluminescent immunoassay on the ARCHITECT i2000SR immunoassay analyzer (Abbott Diagnostics, Abbott Park, IL, USA).
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8

Comprehensive Blood Cell Analysis

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All CBC analyses were performed with an automatic hematologic analyzer (Beckmancoulter DxH 800 Hematology Analyzer). Hemoglobin (Hb), white blood cell (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and platelet counts were obtained before surgical treatment. Blood samples were collected in di-potassium ethylenediaminetetraacetic acid tubes.
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9

Comprehensive Biochemical Profiling in Hospital Admission

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All biochemical procedures were done on the first day of hospital admission in a specialized biochemical laboratory of the Clinical Center Kragujevac, Serbia. Complete blood cell count (CBC) was measured using a hematology analyzer (DxH 800 Hematology Analyzer by Beckman Coulter). The biochemical parameters such as glucose, creatinine, urea, cholesterol, triglyceride (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT) gamma-glutamyl transferase (GGT), lactate dehydrogenase (LDH), total and direct bilirubin, C-reactive protein (CRP), sodium, and potassium were estimated from the serum samples by using standard kits in an automatic clinical chemistry analyzer (AU680 Clinical Chemistry Analyzer by Beckman Coulter). Measurement of the vitamin D level was performed using an automated immunoassay analyzer—the Alinity i system (Abbott Laboratories, IL, USA) that utilizes the chemiluminescent microparticle immunoassay (CMIA) principle. The level of procalcitonin in the serum was determined by the method of electrochemiluminescence, on the immunochemistry analyzer (Cobas e 411 by Roche). D dimer concentration measurement was performed on coagulation analyzer ACL-TOP 300 (Instrumentation Laboratory, Bedford, USA) employing the automated latex-enhanced particle immunoturbidimetric method.
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10

COVID-19 Infection Parameters Study

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A total of 1250 patients admitted for COVID-19 infection were considered for this study. After applying exclusion criteria, a total of 500 patients meeting the inclusion criteria were included in the study. The parameters measured in this study included age, gender, comorbidities, the medication used for hypertension, laboratory parameters, HRCT score, outcome of the patient. The DxH 800 hematology analyzer (Beckman Coulter, South Drive, IN) was used to assess the whole blood count, while the Vitros 5600 was used to examine all inflammatory markers, renal function tests, and liver function tests (Ortho Clinical Diagnostics, Raritan, NJ). Within 48 hours of admission, all of the patients had a high-resolution computed tomography (HRCT) scan. Comorbidities such as hypertension, diabetes, and bronchial asthma were screened in all recruited patients. All of the patients were monitored until they were cured or died. Figure 1 shows the flowchart of methodology for this study.
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