Bay 60 6538

Female C57BL/6 (B6), IFN-γ^-/-, and TCR-δ^-/- mice on the B6 background, purchased from Jackson Laboratory (Bar Harbor, ME), were housed and maintained in the animal facilities of the University of California, Los Angeles and were used at 12–16 weeks of age. Experimental protocols were approved by the Institutional Animal Care and Use Committee of University of California Los Angeles (Protocol number: ARC#2014-029-03A). Recombinant murine IL-1 and IL-23 were purchased from R & D (Minneapolis, MN). Fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, or allophycocyanin (APC)-conjugated mouse monoclonal antibodies (mAbs) against mouse CD73 (Clone TY/11.8), CD44 (Clone IM7), CD86 (clone GL-1), mouse MHC class II antigen (Clone: M5/114.15.2), αβ T cell receptor (TCR, clone H57-597), or γδ TCR (clone GL3) and isotype control antibodies were purchased from Biolegend (San Diego, CA). The selective A2BR agonist BAY 60–6538 and the selective A2BR antagonist MRS 1754 were purchased from Sigma-Aldrich (St. Louis, MO, USA) and were dissolved as a 1 mM stock solution in DMSO and diluted 1/10000 in culture medium before use.

Female C57BL/6 (B6) and TCR-δ^-/- mice on the B6 background, purchased from Jackson Laboratory (Bar Harbor, ME), were housed and maintained in the animal facilities of the University of Southern California. Recombinant murine IL-1, IL-7, and IL-23 were purchased from R & D (Minneapolis, MN). Fluorescein isothiocyanate (FITC)-, phycoerythrin (PE)-, or allophycocyanin (APC)-conjugated antibodies (Abs) against mouse CD73, CD44, αβ T cell receptor (TCR), or γδ TCR and their isotype control antibodies were purchased from Biolegend (San Diego, CA). (PE)-conjugated anti-mouse A2AR monoclonal antibody was purchased from Santa Cruz Biotechnology (Dallas, Texas). The non-selective AR agonist 50-N-ethylcarboxamidoadenosine (NECA), selective A2AR agonist 2-p-(2-carboxyethyl) phenethylamino-5′-N-ethylcarboxamidoadenosine (CGS21680), selective A2AR antagonist (SCH 58261), selective A2BR agonist (BAY 60-6538), and selective A2BR antagonist (MRS 1754), and the CD73 inhibitor α,β-methylene ADP (APCP) were purchased from Sigma-Aldrich (St. Louis, MO, USA).