Seahorse analyzer xf hs mini xfp software

Manufactured by Agilent Technologies

The Seahorse Analyzer XF HS Mini/XFp software is a tool designed to measure cellular metabolism and bioenergetics. It provides real-time analysis of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in live cells, allowing researchers to gain insights into cellular processes and function.

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Lab products found in correlation

Seahorse xf assay media, by Agilent Technologies (2 mentions) Real time atp rate assay, by Agilent Technologies (2 mentions) Oligo, by Agilent Technologies (1 mentions) Rot aa, by Agilent Technologies (1 mentions)

2 protocols using seahorse analyzer xf hs mini xfp software

Real-Time ATP Measurement in Cells

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Cells were plated at the optimal densities in the Seahorse XF 8-well plates 48/72 h before the measurement, subjected to the FF treatment for 24/48 h and incubated in the Seahorse XF Assay Media at 37 °C for 1 h without CO₂, immediately before starting the Real-Time ATP Rate Assay (Seahorse Bioscience; North Billerica, MA, USA, 1 μM Oligo; 1 μM/0.5 μM Rot/AA). OCR and ECAR measurements were performed with the Seahorse Analyzer XF HS Mini/XFp software and normalized to cell numbers.

Warzecha K.W., Pudełek M., Catapano J., Madeja Z, & Czyż J. (2022). Long-Term Fenofibrate Treatment Stimulates the Phenotypic Microevolution of Prostate Cancer Cells In Vitro. Pharmaceuticals, 15(11), 1320.

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Real-Time ATP Rate Assay in Cells

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Cells were plated in Seahorse XF eight-well plates 48/72 h before the measurement, subjected to DCX/MET/FF for 24/48 h, and incubated in Seahorse XF Assay Media at 37 °C for 1 h without CO₂ immediately before starting the Real-Time ATP Rate Assay (Seahorse Bioscience; 1 µM oligomycin; 1 µM/0.5 µM rotenone/antimycin A). Oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) measurements were performed and analyzed with the Seahorse Analyzer XF HS Mini/XFp software and normalized to cell number.

Catapano J., Luty M., Wróbel T., Pudełek M., Piwowarczyk K., Kędracka-Krok S., Siedlar M., Madeja Z, & Czyż J. (2022). Acquired drug resistance interferes with the susceptibility of prostate cancer cells to metabolic stress. Cellular & Molecular Biology Letters, 27, 100.

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