A complete blood count on blood samples was performed at the Pathology Phenotyping Core at Johns Hopkins University School of Medicine. Tail vein blood was collected using EDTA-coated blood collection tubes (Sarstedt, Nümbrecht, Germany) and analyzed using Procyte Dx analyzer (IDEXX Laboratories, Westbrook, ME, USA).
Edta coated blood collection tubes
Manufactured by Sarstedt
Sourced in Germany
EDTA-coated blood collection tubes are used to collect and store blood samples for various laboratory analyses. The tubes are coated with the anticoagulant EDTA, which prevents the blood from clotting during the collection and storage process.
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Lab products found in correlation
7 protocols using edta coated blood collection tubes
1
Complete Blood Count Analysis
2
Comprehensive Blood Cell Analysis
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A complete blood count on blood samples was performed at the Pathology Phenotyping Core at Johns Hopkins University School of Medicine. Tail vein blood was collected using EDTA-coated blood collection tubes (Sarstedt, Nümbrecht, Germany) and analyzed using Procyte Dx analyzer (IDEXX Laboratories, Westbrook, ME).
3
Complete Blood Count Analysis
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A complete blood count on blood samples was performed at the Pathology Phenotyping Core at Johns Hopkins University School of Medicine. Tail vein blood was collected using EDTA‐coated blood collection tubes (Sarstedt, Nümbrecht, Germany) and analyzed using Procyte Dx analyzer (IDEXX Laboratories, Westbrook, ME).
4
Comprehensive Blood Cell Analysis
5
Bilateral Nephrectomy Rat Model of AKI
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Bilateral nephrectomy was performed to model AKI. Rats were divided into two groups, a bilateral nephrectomy group (BNex, n = 11) and a sham group (BNex sham, n = 6). The animals were bilaterally nephrectomised in one surgical session, as described previously [34 (link)]. Animals were anesthetized by ketamine (100 mg/kg, Richter Pharma AG, Wels, Austria) and xylazine (10 mg/kg, Ecuphar N.V., Oostkamp, Belgium) administered by intraperitoneal injection. A midline incision was made in the BNex group; the kidneys were exposed and decapsulated. Renal pedicles were tied off with a suture, and the kidneys were removed. The incision was closed with an absorbable suture. Animals in the sham group underwent sham surgery. Both kidneys of the rats in this group were decapsulated. Animals were sacrificed 48 hours after surgery, under general anesthesia. Blood was collected into EDTA-coated blood collection tubes (Sarstedt, Numbrecht, Germany) from the abdominal aorta. Plasma was obtained by centrifugation (5000 g for 5 minutes) and stored at -20°C until further analysis.
6
Fasting Blood and Tissue Collection
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Fasting Blood was collected in EDTA-coated blood collection tubes (Sarstedt, Newton, NC, 16.44.100). Terminal blood collection was performed after euthanasia via cardiac puncture. Blood was put into heparin-coated tubes and was centrifuged at 14,000 g 4 °C for 10 min. Plasma was collected and stored at −80 °C until analysis. Tissues were snap-frozen in liquid nitrogen and were stored at −80 °C until analysis.
7
Biological Sampling from Mice
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To obtain plasma samples, mice were anesthetized using the isoflurane drop-method, by placing the mice briefly in a closed jar with a cotton pad wetted with several drops of non-diluted isoflurane. Blood was collected from the retro-orbital plexus into two 500 µl EDTA coated blood collection tubes (up to 250 µl of blood into each tube) (Sarstedt, Numbrecht, Germany). Plasma was obtained by centrifugation (2,000 g for 7 min), plasma samples were stored at -20 °C. Urine samples were collected during 4 h of fasting in metabolic cages, to the volume of 800 µl on average, and stored at -20 °C prior to analysis. For saliva collection, mice were anesthetized with intraperitoneal injection of ketamine (100 mg/kg, Richter Pharma AG, Wels, Austria) and xylazine (10 mg/kg, Ecuphar N. V., Oostkamp, Belgium). Salivation was induced using pilocarpine (0.5 µg/kg, Unimed Pharma, Bratislava, Slovakia). Saliva samples were collected for 30 min, to the volume of 400 µl, approximately, and stored at -20 °C prior to analysis.
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