Aggregate solutions were diluted fifty-fold at 20 °C to generate 0.10% w/w dispersions. Copper/palladium TEM grids (Agar Scientific) were surface-coated in-house to yield a thin film of amorphous carbon. The grids were then plasma glow-discharged for 30 s to create a hydrophilic surface. Individual samples (0.1% w/w, 12 μL) were adsorbed onto the freshly glow-discharged grids for 1 min and then blotted with filter paper to remove excess solution. To stain the aggregates, uranyl formate (0.75% w/v) solution (9 μL) was soaked on the sample-loaded grid for 20 s and then carefully blotted to remove excess stain. The grids were then dried using a vacuum hose. Imaging was performed on a Phillips CM100 instrument at 100 kV, equipped with a Gatan 1k CCD camera.
Cm 100 instrument
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The CM 100 instrument is a laboratory equipment product designed for analytical purposes. It serves as a precision measurement device, providing accurate data and information within its core functionality. The detailed specifications and intended applications of this product are not available for an unbiased and factual presentation at this time.
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Lab products found in correlation
7 protocols using cm 100 instrument
1
TEM Imaging of Nanoparticle Aggregates
2
Positive Staining for Diblock Copolymer TEM
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TEM studies were conducted using a Philips CM 100 instrument operating at 100 kV and equipped with a Gatan 1 k CCD camera. Diluted diblock copolymer dispersions (0.10% w/w) were placed as droplets on carbon-coated copper grids, allowed to dry and then exposed to ruthenium(viii ) oxide vapor for 7 min at 20 °C prior to analysis. This heavy metal compound acted as a positive stain for the core-forming PBzMA block in order to improve electron contrast. The ruthenium(viii ) oxide was prepared as follows. Ruthenium(iv ) oxide (0.30 g) was added to water (50 g) to form a black slurry; addition of sodium periodate (2.0 g) with stirring produced a yellow solution of ruthenium(viii ) oxide within 1 min.78
3
Transmission Electron Microscopy of Diblock Copolymers
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TEM studies
were conducted using a Philips CM 100 instrument operating at 100
kV and equipped with a Gatan 1 k CCD camera. A single droplet of a
0.10% w/w diblock copolymer dispersion was placed onto a carbon-coated
copper grid using a pipet and allowed to dry, prior to exposure to
ruthenium(VIII) oxide vapor for 7 min at 20 °C.29 (link) This heavy metal compound acted as a positive stain for
the core-forming PTFEMA block to improve contrast. The ruthenium(VIII)
oxide was prepared as follows: ruthenium(IV) oxide (0.30 g) was added
to water (50 g) to form a black slurry; the addition of sodium periodate
(2.0 g) with continuous stirring produced a yellow solution of ruthenium(VIII)
oxide within 1 min at 20 °C.
were conducted using a Philips CM 100 instrument operating at 100
kV and equipped with a Gatan 1 k CCD camera. A single droplet of a
0.10% w/w diblock copolymer dispersion was placed onto a carbon-coated
copper grid using a pipet and allowed to dry, prior to exposure to
ruthenium(VIII) oxide vapor for 7 min at 20 °C.29 (link) This heavy metal compound acted as a positive stain for
the core-forming PTFEMA block to improve contrast. The ruthenium(VIII)
oxide was prepared as follows: ruthenium(IV) oxide (0.30 g) was added
to water (50 g) to form a black slurry; the addition of sodium periodate
(2.0 g) with continuous stirring produced a yellow solution of ruthenium(VIII)
oxide within 1 min at 20 °C.
4
Nanoparticle Adsorption and Imaging
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Copper/palladium TEM grids (Agar Scientific, UK) were surfacecoated with a thin film of amorphous carbon. Each grid was exposed to a plasma glow discharge for 30 s to produce a hydrophilic surface. One 10 mL droplet of a 0.10% w/w aqueous dispersion was carefully placed onto a grid using a micropipet and left for 1 min before blotting to remove excess liquid. The adsorbed nanoparticles were then stained using a 0.75% w/w aqueous solution of uranyl formate (9.0 mL) for 20 s before blotting to remove excess stain. Each grid was dried under vacuum and images were recorded at 100 kV using a Philips CM100 instrument equipped with a Gatan 1 k CCD camera.
5
Visualizing Block Copolymer Structures via TEM
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Transmission electron microscopy (TEM) studies were conducted using a Philips CM 100 instrument operating at 100 kV and equipped with a Gatan 1k CCD camera. Diluted block copolymer dispersions (0.50% w/w) were placed on carbon-coated copper grids and exposed to either ruthenium tetraoxide vapor for 7 minutes at 20 °C or 0.75% w/w uranyl formate solution (9 μL) was soaked on the sample-loaded grid for 20 seconds. In each case the heavy metal acts as a stain to improve contrast. The ruthenium(viii ) oxide was prepared as follows: ruthenium(iv ) oxide (0.30 g) was added to water (50 g) to form a black slurry; addition of sodium periodate (2.0 g) with stirring produced a yellow solution of ruthenium(viii ) oxide within 1 min at 20 °C.
6
Visualizing Block Copolymer Structures via TEM
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Transmission
electron microscopy (TEM) studies were conducted using a Philips CM
100 instrument operating at 100 kV and equipped with a Gatan 1 k CCD
camera. Diluted diblock copolymer solutions (0.10% w/w) were placed
as droplets on carbon-coated copper grids, exposed to ruthenium(VIII)
oxide vapor for 7 min at 20 °C, and dried prior to analysis.47 (link) The ruthenium(VIII) oxide solution was prepared
as follows: ruthenium(IV) oxide (0.3 g) was added to water (50 g)
to form a black slurry; addition of sodium periodate (2.0 g) with
stirring produced a yellow solution of ruthenium(VIII) oxide within
1 min. This heavy metal compound acted as a positive stain for the
core-forming PBzMA block in order to improve contrast.
electron microscopy (TEM) studies were conducted using a Philips CM
100 instrument operating at 100 kV and equipped with a Gatan 1 k CCD
camera. Diluted diblock copolymer solutions (0.10% w/w) were placed
as droplets on carbon-coated copper grids, exposed to ruthenium(VIII)
oxide vapor for 7 min at 20 °C, and dried prior to analysis.47 (link) The ruthenium(VIII) oxide solution was prepared
as follows: ruthenium(IV) oxide (0.3 g) was added to water (50 g)
to form a black slurry; addition of sodium periodate (2.0 g) with
stirring produced a yellow solution of ruthenium(VIII) oxide within
1 min. This heavy metal compound acted as a positive stain for the
core-forming PBzMA block in order to improve contrast.
7
TEM Imaging of Block Copolymers
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Transmission electron microscopy (TEM) studies were conducted using a Philips CM 100 instrument operating at 100 kV and equipped with a Gatan 1k CCD camera. Diluted block copolymer solutions (0.10% w/w) were placed on carboncoated copper grids and exposed to ruthenium(VIII) oxide vapor for 7 min at 20 1C prior to analysis. 42 This heavy metal compound acted as a positive stain for the core-forming PBzMA block to enhance electron contrast. The ruthenium(VIII) oxide was prepared as follows: ruthenium(IV) oxide (0.30 g) was added to water (50 g) to form a black slurry; addition of sodium periodate (2.0 g) with stirring produced a yellow solution of ruthenium(VIII) oxide within 1 min.
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