Hepes ph 8

Cell lines were kept in a humidified incubator at 37 °C with 5% CO₂ unless otherwise denoted. SKW-3 (derived from a male with T cell leukaemia), Jurkat T cells (derived from a male with acute T cell leukaemia), Raji B lymphocytes (derived from a male with Burkitt’s lymphoma), THP-1 monocyte cell line (derived from a male with acute monocytic leukaemia), K562 cells and primary human T cells were cultured in RPMI supplemented with 2 mM GlutaMAX (Invitrogen), 10% FBS (Sigma),10 mM HEPES pH 8.0 (Thermo Fisher), 1 mM sodium pyruvate (Gibco) and 50 U ml⁻¹ penicillin and streptomycin (Thermo Fisher). SKW-3 cells were purchased from DSMZ. Jurkat T cells, Raji, THP-1 and K562 cells were purchased from ATCC. Validation of T cell lines was performed by staining with known markers pre- and post-transfection or transduction. HEK293T (LentiX) cells (female-derived kidney cell line) were grown in DMEM complete media (Thermo Fisher) supplemented with 10% FBS, 2 mM L-glutamine, and 50 U ml⁻¹ of penicillin and streptomycin. MC38 cells were purchased from Kerafast, and cultured in DMEM complete media containing 10% FBS, 2 mM L-glutamine, 0.1 mM NEAA, 1 mM sodium pyruvate, 10 mM HEPES, 50 U ml⁻¹ penicillin/streptomycin and 50 μg ml⁻¹ gentamycin sulfate. Cell lines tested negative for mycoplasma (MycoAlert Mycoplasma Detection kit, Lonza).