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Hematrue analyzer

Manufactured by Heska
Sourced in United States

The HemaTrue analyzer is a compact, automated hematology analyzer designed for in-clinic use. It provides complete blood count (CBC) and related parameter measurements from a small sample volume. The HemaTrue analyzer delivers reliable, consistent results to support veterinary healthcare decision-making.

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3 protocols using hematrue analyzer

1

Hematology and Serum Analysis of Infected Mice

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Three groups were analyzed by one-way analysis of variance (ANOVA); uninfected control (n = 3), euthanized due to disease (n = 6), and infected (asymptomatic) survivors (n = 3). Hematology was performed on whole blood collected in EDTA (Becton–Dickinson, Franklin Lakes, NJ, USA) using a Heska HemaTrue Analyzer. Blood collected into SST tubes (Becton-Dickinson) was centrifuged at 1000× g for 10 min at 4 °C and serum analyzed with a Heska DRI-CHEM 4000 chemistry analyzer (Heska, Loveland, CO, USA).
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2

Metabolic Profiling of Mouse Aquaresis

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Single mice were kept for 24-hours in metabolic cages (3600M021, Techniplast) and urine and stool production, as well as water and food intake were measured. Treatments were performed with dDAVP (0.4μg/kg intraperitoneally) and effects on these parameters were assessed. Mouse serum chemistries were determined with a Dri-Chem7000 chemistry analyzer (Heska). Urine chemistries were determined with a Roche ModP analyzer and urine osmolality with an Advanced Instruments Micro Osmometer Model 3300. CBC was obtained with the HemaTrue analyzer (Heska). Blood gas analysis was performed with an IRMA TRUpoint capillary blood analysis system (Lifehealth). Mouse serum renin was measured with a mouse renin 1 ELISA kit (EMREN1, Thermo Fisher), according to the manufacturer’s instructions. Aldosterone assays were performed with a commercially available radioimmunoassay kit (ACTIVE® Aldosterone RIA, Beckman Coulter, IMMUNOTECH, Prague, Czech Republic). Anti-aldosterone antibody-coated tubes, 125I-labeled aldosterone radioligand, 7 calibrators ranging from 0 to 4448 pmol/L, and 2 controls were provided by the manufacturer. The assays were performed according to the kit protocol with the modification of an overnight incubation step at room temperature.
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3

Quantifying Leukocytes in Animal Blood

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Total and differential white blood cell counts were used to quantify the leukocytes present in the animal’s blood. Total white blood cell count was obtained using a HemaTrue analyzer (Heska SN 61372) in Wildlife Safari’s veterinary laboratory. The cow setting was used for cervids and bovids while the horse setting was used for zebra. Differential counts were performed from a blood smear slide made on the day of capture and stained with Diff-Quik. Absolute numbers of each leukocyte type were determined by multiplying the total white blood cell count by the differential percentages.
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