Cells were fixed with 4% paraformaldehyde, blocked with 1% bovine serum albumin, and incubated overnight with ZNF452 and myc-tag monoclonal antibodies (1:100; Santa cruz) at 4°C. Then, the cells were incubated with tetramethylrhodamine isothiocyanate-conjugated secondary antibodies (Cell Signaling Technology) at 37°C for 2 h; cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Epifluorescence microscopy was performed using an inverted Nikon TE300 microscope (Nikon Co., Ltd., Tokyo, Japan), and confocal microscopy was performed using a Radiance 2000 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
Tetramethylrhodamine isothiocyanate conjugated secondary antibodies
Manufactured by Cell Signaling Technology
Tetramethylrhodamine isothiocyanate-conjugated secondary antibodies are fluorescently labeled antibodies used in immunoassays and other applications. They are designed to bind to primary antibodies and emit a red-orange fluorescent signal upon excitation, allowing for detection and visualization of target proteins or molecules.
Automatically generated - may contain errors
5 protocols using tetramethylrhodamine isothiocyanate conjugated secondary antibodies
1
Immunofluorescence Imaging of ZNF452 and Myc-Tag
2
Immunofluorescence Staining of FAM98A
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were washed with PBS, fixed with 4% paraformaldehyde, and blocked with 5% BSA for 1 h. Afterward, the cells were incubated overnight with FAM98A antibodies (1:50) at 4°C. Cells were washed with PBS and incubated with tetramethylrhodamine isothiocyanate-conjugated secondary antibodies (Cell Signaling Technology) at 37°C for 2 h. Cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole. Epifluorescence microscopy was performed using an inverted Nikon TE300 microscope (Nikon Co., Ltd., Tokyo, Japan), and confocal microscopy was performed using a Radiance 2000 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
3
Immunofluorescence Imaging of THUMPD1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4% paraformaldehyde, blocked with 1% bovine serum albumin, and incubated overnight with THUMPD1 monoclonal antibodies (1:100; Santa Cruz) at 4°C. Cells were then incubated with tetramethylrhodamine isothiocyanate-conjugated secondary antibodies (Cell Signaling Technology) at 37°C for 2 h. Cell nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI). Epifluorescence microscopy was performed using an inverted Nikon TE300 microscope (Nikon Co., Ltd., Tokyo, Japan), and confocal microscopy was performed using a Radiance 2000 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
4
Immunofluorescence Staining and Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4% paraformaldehyde, blocked with 1% bovine serum albumin, and incubated overnight with Lasp1, p-FAK, p-AKT, FAK, Myc-tag antibodies (1:100) at 4°C. Cells were then incubated with tetramethylrhodamine isothiocyanate-conjugated secondary antibodies (Cell Signaling Technology) at 37°C for 2 h. Cell nuclei were counterstained with 4′, 6-diamidino-2-phenylindole (DAPI). Epifluorescence microscopy was performed using an inverted Nikon TE300 microscope (Nikon Co., Ltd., Tokyo, Japan), and confocal microscopy was performed using a Radiance 2000 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
5
Immunofluorescence Analysis of Epithelial Cell Junctions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed with 4% paraformaldehyde, blocked with 1% bovine serum albumin, and incubated overnight with E-cadherin, occludin, and ZO-1 antibodies (1:100; BD Biosciences and Proteintech) at 4°C. Then, the cells were incubated with tetramethylrhodamine isothiocyanate-conjugated secondary antibodies (Cell Signaling Technology) at 37°C for 2 h; cell nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Epifluorescence microscopy was performed using an inverted Nikon TE300 microscope (Nikon Co., Ltd, Tokyo, Japan), and confocal microscopy was performed using a Radiance 2000 laserscanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!