Tet ofuw myt1l

pMXS-Oct4, pMXS-Sox2, pMXS-Klf4, pMXS-Myc, pLKO.1 and pWPXLd plasmids were purchased from Addgene. Short hairpin RNAs (shRNAs) against Trp53, FosL1, Atoh8 and Sfrp1 were designed using the MISSION shRNA library from Sigma–Aldrich and ligated using the Rapid DNA ligation kit (Sigma–Aldrich) into the pLKO.1 vector digested with AgeI and EcoRI. Supplementary Table 2 lists the shRNA sequences. Atoh8 and Bcl11b complementary DNA was amplified from MEFs and cloned into the pWPXLd expression vector at the BamH1 restriction site. For Atoh8 ChIP-Seq, AM-Tag was added at the carboxy terminal. Single guide RNA targeting Atoh8 (designed using the CRISPOR program) was cloned into the lentiCRISPRv2 plasmid at a BsmBI restriction site. The single guide RNA sequences are listed in Supplementary Table 2. The pWPIR H-ras G12V and cyclin E plasmids were kindly supplied by the laboratory of A. Puisieux. Tet-O-FUW-Brn2, Tet-O-FUW-Ascl1, Tet-O-FUW-Myt1l, FUW-TetO-Sall4, FUW-TetO-Nanog, FUW-TetO-Esrrb, FUW-TetO-Lin28 and FUdeltaGW-rtTA plasmids were purchased from Addgene.