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Puseamp containing a myc tag

Manufactured by Merck Group
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PUSEamp containing a MYC tag is a laboratory equipment product manufactured by Merck Group. It serves as a tool for protein expression and purification. The MYC tag is a short peptide sequence that can be attached to a protein of interest, enabling its detection and affinity-based purification.

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Lab products found in correlation

Pcdna3.1 s1pr1, by OriGene (2 mentions) Pcmv6 sphk1, by OriGene (2 mentions) Lipofectamine, by Thermo Fisher Scientific (2 mentions) Nucleofection, by Lonza (2 mentions)

Close spelling variants of this product

Myc-tag (34 citations) PUSEamp- (2 citations)

2 protocols using puseamp containing a myc tag

1

Molecular Manipulation of B Cell Signaling

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GC B cells were nucleofected with pcDNA3.1-PRDM1 or empty vector, cultivated and enriched as before.20 (link), 21 (link) HL cell lines and DG75 cells were transfected by Nucleofection (Lonza, Basel, Switzerland) and HEK293 cells were transfected by Lipofectamine (ThermoFisher Scientific) using the following vectors and corresponding controls: pcDNA3.1-S1PR2-HA (UMR cDNA Resource Center University of Missouri-Rolla, Rolla, MO, USA), pSG5-myc-p110α-CAAX (Julian Downward, Addgene, Cambridge, MA, USA), Akt1/PKBα cDNA (activated) in pUSEamp containing a MYC tag (Merck Millipore, Billerica, MA, USA), pcDNA3.1- S1PR1 (Dr Ian Paterson, University of Malaya, Kuala Lumpur, Malaysia) and pCMV6-SPHK1 (Origene technologies, Rockville, MD, USA).
Vrzalikova K., Ibrahim M., Vockerodt M., Perry T., Margielewska S., Lupino L., Nagy E., Soilleux E., Liebelt D., Hollows R., Last A., Reynolds G., Abdullah M., Curley H., Care M., Krappmann D., Tooze R., Allegood J., Spiegel S., Wei W., Woodman C.B, & Murray P.G. (2017). S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells. Leukemia, 32(1), 214-223.
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2

Genetic Manipulation of Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
GC B cells were nucleofected with pcDNA3.1-PRDM1 or empty vector, cultivated and enriched as before.20 (link),21 (link) HL cell lines and DG75 cells were transfected by nucleofection (Lonza, Basel, Switzerland) and HEK293 transfected by lipofectamine (ThermoFisher Scientific) using the following vectors and corresponding controls: pcDNA3.1-S1PR2-HA (UMR cDNA Resource Center University of Missouri-Rolla, MO, USA), pSG5-myc-p110α-CAAX (Julian Downward, Addgene, Cambridge, MA, USA), Akt1/PKBα cDNA (activated) in pUSEamp containing a MYC tag (Merck Millipore, Billerica, MA, USA), pcDNA3.1-S1PR1 (Dr Ian Paterson, University of Malaya) and pCMV6-SPHK1 (Origene technologies, Rockville, MD, USA).
Vrzalikova K., Ibrahim M., Vockerodt M., Perry T., Margielewska S., Lupino L., Nagy E., Soilleux E., Liebelt D., Hollows R., Last A., Reynolds G., Abdullah M., Curley H., Care M., Krappmann D., Tooze R., Allegood J., Spiegel S., Wei W., Woodman C.B, & Murray P.G. (2017). S1PR1 drives a feedforward signalling loop to regulate BATF3 and the transcriptional programme of Hodgkin lymphoma cells. Leukemia, 32(1), 214-223.
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