Luciferase fluorescence intensity was measured by the IVIS imaging system (PerkinElmer). Mice were anesthetized with a mixture of isoflurane and oxygen, and then intraperitoneally (i.p.) injected with D-luciferin (15 mg/ml, GoldBio no. LUCNA-100). 5 min after the D-luciferin injection, mice were imaged with IVIS imaging system. Quantitative analysis of imaging signals (luminescence counts) was processed by Living Imaging software (PerkinElmer).
Lucna 100
Manufactured by GoldBio
The LUCNA-100 is a laboratory instrument designed for the purification and analysis of nucleic acids. It utilizes a proprietary technology to perform high-efficiency extraction and purification of DNA and RNA samples from a variety of sources. The core function of the LUCNA-100 is to provide consistent and reliable results for downstream applications such as PCR, sequencing, and other molecular biology techniques.
Automatically generated - may contain errors
2 protocols using lucna 100
1
In Vivo Bioluminescence Imaging
2
Bioluminescent Imaging of Rhabdomyosarcoma Tumor Growth
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RMS cell-injected mice were assessed daily following implantation, and tumor growth/recession was monitored using an IVIS spectrum in vivo imaging system (PerkinElmer). For these assays, mice were sedated with isoflurane, i.p. injected with approximately 100 µl of 30 mg/ml luciferin (150 mg/kg, d -Luciferin sodium salt, Gold Biotechnology, LUCNA-100), and imaged for bioluminescence detection after 15 min under sedation. RMS cell-injected mice were further assessed for bioluminescence using the IVIS directly following three/five days of radiation treatment (afternoon of the last treatment) and the beginning of the following week to assess tumor volume change, then weekly thereafter to monitor tumor growth/recurrence. The mice were then euthanized. Any mice with substantial tumor regrowth were euthanized at two weeks post-radiation treatment, or sooner if their condition declined.
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