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Anti cd57

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Anti-CD57 is a monoclonal antibody used to detect the CD57 antigen, also known as HNK-1 or Leu-7. CD57 is a carbohydrate epitope expressed on a subset of human natural killer (NK) cells, cytotoxic T cells, and a small population of B cells. This antibody can be used in flow cytometry and other immunoassays to identify and characterize CD57-positive cells.

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Lab products found in correlation

Anti tim 3, by R&D Systems (2 mentions) Anti cd14, by BD (2 mentions) Anti nkg2c, by R&D Systems (1 mentions) Anti cd56, by BD (1 mentions) Anti cd3, by Thermo Fisher Scientific (1 mentions) Anti cd16, by BioLegend (1 mentions) Anti pd 1, by BioLegend (1 mentions) Anti tim 3, by BioLegend (1 mentions) Anti granzyme b, by BioLegend (1 mentions) Anti cxcr3, by BioLegend (1 mentions) Anti human lineage cocktail, by BioLegend (1 mentions) Anti cd27, by BioLegend (1 mentions) Anti tigit, by BioLegend (1 mentions) Anti nkp44, by BioLegend (1 mentions) Anti nkg2d, by BioLegend (1 mentions) Anti cd69, by BioLegend (1 mentions) Anti ki67, by BioLegend (1 mentions) Anti trail, by BioLegend (1 mentions) Anti perforin, by BioLegend (1 mentions) Anti cx3cr1, by BioLegend (1 mentions)

4 protocols using anti cd57

1

Immune Receptor Repertoire Analysis

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Peripheral blood mononuclear cells (PBMCs) were isolated from blood by density centrifugation and were cryopreserved with 5% DMSO. Samples were thawed and rested overnight prior to flow cytometry. Immune receptor repertoires were determined by flow cytometry on an LSRII (BD Biosciences). PBMCs were analyzed for NK cells (CD3-CD56+), and adaptive (CD56dimCD57+NKG2C+) NK cells using fluorescently conjugated antibodies: anti-CD3 (Invitrogen; MHCD0317), anti-CD56 (BD Biosciences; 335791), anti-NKG2C (R&D; FAB138P), anti-CD57 (Biolegend; 322316). Data analysis was performed using FlowJo 9.3.2 software (TreeStar).
Merino A.M., Mehta R.S., Luo X., Kim H., De For T., Janakiram M., Cooley S., Wangen R., Cichocki F., Weisdorf D.J., Miller J.S, & Bachanova V. (2020). Early Adaptive NK Cell Expansion is Associated with Decreased Relapse After Autologous Transplant for Multiple Myeloma. Transplantation and cellular therapy, 27(4), 310.e1-310.e6.
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2

Comprehensive NK Cell Phenotyping

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The following fluorescently conjugated antibodies were used for phenotypic analysis of NK cells: anti-LFA-1 (363404); anti-CXCR3 (353720); anti-PD-1 (329906); anti-CD107a (328612); anti-NKP44 (325116); anti-CD158e1 (312706); anti-CD158d (347006); anti-CD27 (356412); anti-CCR4 (359412); anti-DNAM-1 (338316); anti-CD16 (302040); anti-Granzyme B (515406); anti-CD62L (304806); anti-CD69 (310910); anti-NKP80 (346706); anti-NKP30 (325210); anti-CD158f (341304); anti-CD158b (312612); anti-Tim-3 (345012); anti-CD94 (305504); anti-TIGIT (372706); anti-TRAIL (308206); anti-CD57 (322306); anti-CX3CR1 (341610); anti-NKG2D (320808); anti-Perforin (353310); anti-Ki67 (350504); anti-IFN-γ (506518); anti-CD94 (305506); anti-NKp46 (331916); anti-CTLA4 (369614); anti-CD96 (338416); anti-41BB (309818); anti-CD25 (356108) were purchased from Biolegend. anti-CD159a/NKG2A (FAB1059P) and anti-NKG2C (FAB138G) were purchased from R&D.
The following fluorescently conjugated antibodies were used to identify immune cell types in eNK or PBMC: anti-human Lineage Cocktail (348803); anti-CD56 (362550); anti-CD3 (300430); anti-CD33 (366620); anti-HLA-DR (307606); anti-CD14 (301836); anti-CD19 (302242); anti-CD11b (301322); anti-CD25 (356108); and anti-FOXP3 (320108) were purchased from Biolegend, San Diego, CA, USA.
Chen M., Li Y., Wu Y., Xie S., Ma J., Yue J., Lv R., Tian Z., Fang F, & Xiao W. (2021). Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer. Cancers, 13(22), 5866.
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3

Comprehensive Multiparametric Flow Cytometry Analysis

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PBMCs were incubated with fluorochrome-conjugated mAbs and analyzed on either an FC 500 (Beckman Coulter) or an LSRFortessa (Becton Dickinson) flow cytometer. FITC-, PE-, PE-Texas Red-, ECD-, APC-, PE-Cy5–, PE-Cy7–, PerCP-Cy5.5–, Pacific Blue-, and AF700-conjugated mouse anti-human mAbs included anti-CD3, anti-CD4, anti-CD8, anti-CD11c, anti-CD14, anti-CD16 (clone 3G8), anti-CD19, anti-CD25, ant-CD28, anti-CD45RA, anti-CD45RO, anti-CD80, anti-CD86, anti-CD123, anti-CTLA-4, anti–HLA-DR, anti-IL2, anti-Ki-67 (BD Pharmingen), anti-CD56, anti-CD83 (Beckman Coulter), anti-CD127, anti- IFNγ, anti-LAG-3, anti-PD-1, anti- TNFα (eBioscience), anti-CCR7, anti-TIM-3 (R&D Systems), and anti-CD57 (BioLegend). Nonreactive isotype-matched antibodies (Becton Dickinson, eBioscience, R&D Systems) were used as controls. LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Life Technologies) facilitated exclusion of dead cells. Gates were set for collection and analysis of at least 20,000 live events. Data were analyzed with FlowJo 9.5 software (TreeStar).
Chung D.J., Pronschinske K.B., Shyer J.A., Sharma S., Leung S., Curran S.A., Lesokhin A.M., Devlin S.M., Giralt S.A, & Young J.W. (2015). T cell exhaustion in multiple myeloma relapse after autotransplant: Optimal timing of immunotherapy. Cancer immunology research, 4(1), 61-71.
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4

Comprehensive immune cell profiling by flow cytometry

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BMMCs were incubated with fluorochrome-conjugated antibodies and analyzed on a Cytek Aurora flow cytometer. BUV395-, BUV496-, BUV563-, BUV805-, BV421-, BV510-, BV650-, BV785-, FITC-, SparkBlue-, BB700-, BB790-, PE-, PE-CF594-, PE-Cy7-, A647-, A700-, and APC-Vio770-conjugated mouse anti-human mAbs included anti-CD3, anti-CD4, anti-CD14, anti-CD16, anti-CD25, anti-CD45RO, anti-CD68, anti–PD-1 (all from BD Biosciences), anti-CD45RA, anti-CD57, anti-CD86, anti-CD127, anti-CCR7, anti-FOXP3, anti–HLA-DR, anti–LAG-3 (all from BioLegend), anti-CD8 and anti-CD56 (Miltenyi Biotec), anti-TIM-3 (R&D Systems), and SYTOX Blue/viability (Thermo Fisher). Gates were set for collection and analysis of ≥50,000 live events. Data analysis, including t-distributed stochastic neighbor embedding plot generation, was performed using FlowJo software (FlowJo LLC).
Chung D.J., Sharma S., Rangesa M., DeWolf S., Elhanati Y., Perica K, & Young J.W. (2022). Langerhans dendritic cell vaccine bearing mRNA-encoded tumor antigens induces antimyeloma immunity after autotransplant. Blood Advances, 6(5), 1547-1558.
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