According to previous established procedures, cells and muscle samples were harvested, washed with 1× phosphate‐buffered saline (PBS), and lysed in NP40 lysis buffer (50 mM Tris–HCl, 150 mM NaCl, 0.1% NP‐40, 5 mM EDTA, 10% glycerol) with protease inhibitors cocktail (Sigma‐Aldrich, St. Louis, MO, USA).18 Proteins were separated in SDS‐PAGE, transferred, and immunoblotted with various antibodies. The antibodies used were anti‐Wnt5a (1:1500; Invitrogen, Carlsbad, CA, USA), anti‐MyoD (1:1500; Invitrogen), anti‐MyoG (1:1500; Invitrogen), anti‐MEF2C (1:1500; Invitrogen), anti‐Myf5 (1:1500; Invitrogen), and anti‐β‐actin (1:3000; Santa Cruz Biotechnology, Dallas, TX, USA).
Anti myog
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-MyoG is a primary antibody product used in laboratory research. It is designed to specifically detect and bind to the MyoG protein, which is a transcription factor involved in muscle cell differentiation. The core function of Anti-MyoG is to facilitate the identification and analysis of MyoG expression in various experimental models and cell types.
Automatically generated - may contain errors
Lab products found in correlation
Anti myod, by Thermo Fisher Scientific (2 mentions)
Protease inhibitors cocktail, by Merck Group (1 mentions)
Anti β actin, by Santa Cruz Biotechnology (1 mentions)
Anti wnt5a, by Thermo Fisher Scientific (1 mentions)
Anti sepp1, by Abcam (1 mentions)
Anti active β catenin, by Merck Group (1 mentions)
Anti β catenin, by Abcam (1 mentions)
Ab32572, by Abcam (1 mentions)
2 protocols using anti myog
1
Protein Expression Analysis in Muscle Cells
2
Protein Expression Analysis Protocol
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To determine protein expression, cells were harvested in RIPA buffer containing a protease inhibitor cocktail, and total protein was quantified using a bicinchoninic acid kit (Pierce, Rockford, IL, USA). Aliquots containing 8 μg total protein were separated by sodium dodecyl sulphate polyacrylamide gel electrophoresis and then electro-blotted onto a 0.45-μm PV membrane (Immobilon™; Merck Millipore, Darmstadt, Germany). The membranes were blocked and probed overnight with the primary antibodies anti-SEPP1 (1:1000, #ab193193; Abcam, USA), anti-MyoD (1: 1500; Invitrogen, Carlsbad, CA, USA), anti-MyoG (1: 1500; Invitrogen, Carlsbad, CA, USA), anti–β-catenin (1:5000, #ab32572; Abcam, USA), and anti–active β-catenin (1:500, #05-665; Merck Millipore).
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