Cells were grown in 60 mm tissue culture dishes and washed twice with PBS. Cells were lysed in NuPAGE sample buffer and heated in boiling water for 5 min. The extracts were fractionated by SDS–PAGE (NuPAGE 4-12% gel, NOVEX, Life technologies, USA) and subjected to Western blotting. The following antibodies were used: anti-cytokeratin 19 (sc-25724, Santa Cruz), cytokeratin 8 (17514-1-AP, Proteintech), cytokeratin 14 (22221-1-AP, Proteintech), anti-EGFR (sc-03, Santa Cruz), anti-actin (sc-1615, Santa Cruz), caspase-3 (9662, Cell Signaling), PARP (556362, BD Bioscience), E1A (05-595, Millipore), E1B 19K (gift from Dr. M. Green) and E1B-55K (gift from Dr. Peter Yaciuk) Abs.
Anti cytokeratin 19 sc 25724
Manufactured by Santa Cruz Biotechnology
Anti-cytokeratin 19 (sc-25724) is an antibody used in laboratory research. It binds to and detects the cytokeratin 19 protein, which is a component of the cytoskeleton in certain cell types.
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Lab products found in correlation
2 protocols using anti cytokeratin 19 sc 25724
1
Immunoblot Analysis of Epithelial Markers
2
Immunoblot Analysis of Epithelial Markers
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Cells were grown in 60 mm tissue culture dishes and washed twice with PBS. Cells were lysed in NuPAGE sample buffer and heated in boiling water for 5 min. The extracts were fractionated by SDS–PAGE (NuPAGE 4-12% gel, NOVEX, Life technologies, USA) and subjected to Western blotting. The following antibodies were used: anti-cytokeratin 19 (sc-25724, Santa Cruz), cytokeratin 8 (17514-1-AP, Proteintech), cytokeratin 14 (22221-1-AP, Proteintech), anti-EGFR (sc-03, Santa Cruz), anti-actin (sc-1615, Santa Cruz), caspase-3 (9662, Cell Signaling), PARP (556362, BD Bioscience), E1A (05-595, Millipore), E1B 19K (gift from Dr. M. Green) and E1B-55K (gift from Dr. Peter Yaciuk) Abs.
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