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Phrodo red zymosan bioparticles conjugate for phagocytosis

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The PHrodo™ Red Zymosan Bioparticles™ Conjugate is a fluorescent reagent designed to measure phagocytosis. The conjugate consists of pH-sensitive dye-labeled zymosan particles that exhibit increased fluorescence upon acidification, allowing for the real-time detection of particle internalization by phagocytic cells.

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Lab products found in correlation

Axiovert 200m fluorescence microscope, by Zeiss (1 mentions) Cd11bhi, by BioLegend (1 mentions) Cytochalasin d, by Merck Group (1 mentions) Gluteraldehyde, by Electron Microscopy Sciences (1 mentions) Pam3csk4, by InvivoGen (1 mentions)

Close spelling variants of this product

PHrodo Red (66 citations) PHrodo Red Zymosan Bioparticles (22 citations) Zymosan (14 citations) PHrodo™ Red Zymosan Bioparticles™ Conjugate (2 citations)

5 protocols using phrodo red zymosan bioparticles conjugate for phagocytosis

1

Isolation and Phagocytic Assay of Tumor-Infiltrating GAMs

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Tumor-infiltrating GAMs were isolated from B6, S100a4−/− and S100a4+/− hosts injected with ×2808 mouse glioma using FACS sorting of CD45hi (#103114, RRID:AB_312979; BioLegend—1:1000), CD11bhi(#101259, RRID:AB_2566568; BioLegend—1:1000) and GFP+ (in case of S100a4−/− vs. S100a4+/−). GAMs were then cultured overnight and incubated with pHrodo™ Red Zymosan Bioparticles™ Conjugate for Phagocytosis (P35364, Thermo Fisher Scientific) for two hours on the following day. Following two-hour incubation with the beads, immunofluorescence images were obtained using the Zeiss Axiovert 200 M fluorescence microscope.
Abdelfattah N., Kumar P., Wang C., Leu J.S., Flynn W.F., Gao R., Baskin D.S., Pichumani K., Ijare O.B., Wood S.L., Powell S.Z., Haviland D.L., Parker Kerrigan B.C., Lang F.F., Prabhu S.S., Huntoon K.M., Jiang W., Kim B.Y., George J, & Yun K. (2022). Single-cell analysis of human glioma and immune cells identifies S100A4 as an immunotherapy target. Nature Communications, 13, 767.
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2

Immune Cell Activation Assay

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LPS - Lipopolysaccharides from Escherichia coli O111:B4, Calcium Chloride solution 1 M in H2O and cytochalasin D from Sigma-Aldrich; EM Grade Paraformaldehyde (PFA) 16% and Gluteraldehyde 8% from Electron Microscopy Sciences; colchicine and colcemide 1 μg/mL in phosphate buffered saline from Biological Industries; TLR agonists/antagonist (in endotoxin-free water) from Invivogen: PAM3CSK4 – TLR2-TLR1 ligand, synthetic bacterial lipopeptide; FSL-1 – TLR2/TLR6 ligand, synthetic mycoplasmal lipoprotein; CL097 – TLR7/8 ligand, imidazoquinoline compound; LPS-RS Ultrapure – TLR4 antagonist, Ultrapure lipopolysaccharide from Rhodobacter sphaeroides; pHrodo™ Red Zymosan Bioparticles® Conjugate for phagocytosis from ThermoFisher Scientific.
Shahar M., Szalat A, & Rosen H. (2021). Pathogenic Stress Induces Human Monocyte to Express an Extracellular Web of Tunneling Nanotubes. Frontiers in Immunology, 12, 620734.
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3

Phagocytosis Assay with Zymosan

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Peritoneal macrophages were co-cultured 2 h at 37°C with pHrodo™ Red Zymosan Bioparticles™ Conjugate for Phagocytosis (Thermofischer Scientific), according to the manufacturer’s instructions. Fluorescence was measured with a plate reader luminometer (Envision, PerkinElmer).
Le Naour A., Prat M., Thibault B., Mével R., Lemaitre L., Leray H., Joubert M.V., Coulson K., Golzio M., Lefevre L., Mery E., Martinez A., Ferron G., Delord J.P., Coste A, & Couderc B. (2019). Tumor cells educate mesenchymal stromal cells to release chemoprotective and immunomodulatory factors. Journal of Molecular Cell Biology, 12(3), 202-215.
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4

Tcf21 Lineage CD45+ Phagocytosis Assay

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Red Zymosan Bioparticle phagocytosis test was performed using pHrodo Red Zymosan Bioparticles Conjugate for Phagocytosis (Invitrogen) following the manufacturer’s manual. Briefly, Tcf21 lineage CD45+ cells and non-Tcf21 lineage CD45+ cells were seeded onto 96 well plates separately or together at a 1:1 ratio and cultured for 24 h. Red Zymosan Bioparticles were then added to the cell culture. Images were captured using an ECHO revolve fluorescence microscope after 3 h of incubation.
Liu Q., Li C., Deng B., Gao P., Wang L., Li Y., Shiri M., Alkaifi F., Zhao J., Stephens J.M., Simintiras C.A., Francis J., Sun J, & Fu X. (2023). Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development. Cell reports, 42(3), 112166.
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5

Tcf21 Lineage CD45+ Phagocytosis Assay

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Red Zymosan Bioparticle phagocytosis test was performed using pHrodo Red Zymosan Bioparticles Conjugate for Phagocytosis (Invitrogen) following the manufacturer’s manual. Briefly, Tcf21 lineage CD45+ cells and non-Tcf21 lineage CD45+ cells were seeded onto 96 well plates separately or together at a 1:1 ratio and cultured for 24 h. Red Zymosan Bioparticles were then added to the cell culture. Images were captured using an ECHO revolve fluorescence microscope after 3 h of incubation.
Liu Q., Li C., Deng B., Gao P., Wang L., Li Y., Shiri M., Alkaifi F., Zhao J., Stephens J.M., Simintiras C.A., Francis J., Sun J, & Fu X. (2023). Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development. Cell reports, 42(3), 112166.
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