Lcms optima grade methanol

HPLC-grade J.T. Baker water, LCMS Optima-grade ammonium formate, LCMS Optima-grade formic acid, and LCMS Optima-grade methanol were purchased from Fisher Scientific (Suwanee, GA). Analytical standard-grade FDCA was purchased from Sigma-Aldrich (St. Louis, MO). Analytical standard-grade HMFA was purchased from Santa Cruz Biotechnology (Dallas, TX). Analytical standard-grade ATCA, 5HMP, HMFG, N2FG, tt-MA, and PMA were purchased from Toronto Research Chemicals (Ontario, Canada). ATCA-[¹³C,¹⁵N₂], HMFG-[¹³C₂,¹⁵N], 5HMP-[²H₃], FDCA-[¹³C₆], N2FG-[²H₃], and HMFA-[¹³C₆] were purchased from Toronto Research Chemicals (Ontario, Canada). tt-MA-[¹³C₆] was purchased from Sigma-Aldrich (St. Louis, MO). PMA-[¹³C₆] was purchased from Cambridge Isotope Laboratories (Tewksbury, MA). Mass Spect Gold Urine was purchased from Golden West Diagnostics (Temecula, CA). ClinChek urine controls for occupational medicines, levels I/II (lyophilized), were purchased from IRIS Technologies International GmbH (Olathe, KS). A human urine pool was collected anonymously following a protocol approved by the CDC Institutional Review Board.

Adenosine (A), 2′-deoxyadenosine (2dA), guanosine (G), 2′-deoxyguanosine (2dG), cytidine (C), 2′-deoxycytidine (2dC), uridine (U), N²-methylguanosine (N2-MG), 7-methlyguanosine (7-MG), and HPLC grade ammonium acetate (>99%) where purchased from Sigma-Aldrich (St. Louis, MO, USA). Internal standards of [¹³C₅] adenosine (AC5), [¹³C₅] guanosine (GC5) and [¹³C₅] cytidine (CC5) were purchased from Toronto Research Chemicals (North York, ON, CAN). LC-MS optima™ grade methanol was purchased from Fisher Scientific (Waltham, MA, USA). Ultra pure water was obtained via a Miili Q advantage 10 (EMD Millipore, Billerica, MA, USA) equipped with a Q guard T2 purification cartridge, quantum TEX polishing cartridge and millipak express 0.22 μm filter.

HPLC-grade heptafluorobutyric acid (HFBA), LCMS Optima^™-grade methanol, AcroSeal^® 7N ammonia in methanol, and synthetic urine were purchased from Fisher Scientific (Suwanee, GA). Reagents 1N sodium hydroxide (NaOH) and 6N hydrochloric acid (HCl) were purchased from Sigma-Aldrich (St. Louis, MO). Trifluoroacetic acid (TFA), pentafluoropropionic anhydride (PFPA), and analytical standard-grade HDA, IPDA, BMAA and TMAO were purchased from Sigma-Aldrich (St. Louis, MO). IPDA-[¹³C, ¹⁵N₂], BMAA-[¹³C, ¹⁵N₂], and TMAO-[¹³C₃] were purchased from IsoSciences (Kings of Prussia, PA), and HDA-[²H₁₂] from Toronto Research Chemicals (Ontario, Canada). Human urine pool was collected anonymously following a protocol approved by the CDC Institutional Review Board.