Axiocam hrm

Manufactured by Zeiss

Sourced in Germany, United States, Canada

The AxioCam HRm is a high-resolution camera designed for microscopy applications. It features a monochrome sensor with a resolution of 1,388 x 1,040 pixels and a pixel size of 6.45 μm. The camera is capable of capturing images with a maximum frame rate of 11 frames per second. It is compatible with a variety of Zeiss microscope systems.

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Lab products found in correlation

Axiovert 200m, by Zeiss (18 mentions) Axiovision 4, by Zeiss (16 mentions) Axiovert 200m microscope, by Zeiss (15 mentions) Axiovision software, by Zeiss (12 mentions) Axio observer z1, by Zeiss (12 mentions) Plan apochromat, by Zeiss (9 mentions) Axio observer a1, by Zeiss (9 mentions) Axioplan 2, by Zeiss (7 mentions) Axiovert 200, by Zeiss (6 mentions) Lsm 700, by Zeiss (6 mentions) Plan apochromatlan, by Zeiss (5 mentions) Axiovision, by Zeiss (5 mentions) Axioplan 2 imaging, by Zeiss (4 mentions) Axiovert zoom, by Zeiss (4 mentions) Axio imager z1 microscope, by Zeiss (4 mentions) Axiovision release 4, by Zeiss (3 mentions) Digitonin, by Merck Group (3 mentions) Lsm 880 meta, by Zeiss (3 mentions) Axiovision le software, by Zeiss (3 mentions) Zen 2011, by Zeiss (3 mentions)

Close spelling variants of this product

AxioCam (641 citations) AxioCam HRm camera (138 citations) AxioCam HRm CCD camera (12 citations) AxioCam HRm CCD (5 citations) AxioCam HRm digital camera (5 citations) AxioCam Hrm Rev 3 (4 citations) AxioCam HRm Rev 3 camera (3 citations) AxioCam HRM camera HAL 100 lamp (3 citations) AxioCam HRm microscope camera (2 citations)

161 protocols using axiocam hrm

Aggregates Degradation Dynamics Monitoring

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For determining degradation of Puromycin-induced p62 aggregates, HeLa cells treated with DMSO or 10 μM Ro-3306 for 24 h were incubated with 5 μg/ml Puromycin for the indicated time. Cells were fixed, immunostained with anti-p62 antibody, and observed with confocal microscopy (LSM 880 Meta plus Zeiss Axiovert Zoom; Zeiss) equipped with a 63×/1.40 oil-immersion objective lens (Plan-Apochromat; Zeiss) and a camera (AxioCam HRm; Zeiss) at RT.
For examining degradation of Htt97Q-GFP aggregates, HeLa cells stably expressing Tet-on Htt97Q-GFP were incubated with 1 μg/ml doxycycline for 10 h to induce Htt97Q-GFP expression. Cells were washed three times with PBS buffer and incubated with DMSO or 10 μM Ro-3306 for the indicated times. Htt97Q-GFP foci were observed with a confocal microscope (LSM 880 Meta plus Zeiss Axiovert Zoom; Zeiss) equipped with a 63×/1.40 oil-immersion objective lens (Plan-Apochromat; Zeiss) and a camera (AxioCam HRm; Zeiss) at RT.

Wang Z., Chen D., Guan D., Liang X., Xue J., Zhao H., Song G., Lou J., He Y, & Zhang H. (2022). Material properties of phase-separated TFEB condensates regulate the autophagy-lysosome pathway. The Journal of Cell Biology, 221(5), e202112024.

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Indirect immunofluorescence analysis of VZV

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Indirect IFA was performed on cell spots of FACS sorted VZV or mock cultured NK cells (CD3^–CD56⁺). Cell spots were air dried on glass slides and fixed with 4% formaldehyde (Cytofix; BD Biosciences) at room temperature. Subsequently, cell spots were permeabilised with 0.1% Triton X-100 (Sigma-Aldrich) for 10 mins at room temperature. Cell spots were blocked with 20% donkey serum, and then stained against IE63 (1:500), pORF29 (1:750), or respective isotype control, for 1 hr at 37°C. Thereafter cell spots were stained with fluorochrome-conjugated secondary antibodies, and finally mounted with ProLong Gold Antifade Mountant with DAPI (Thermo Fisher Scientific). Imaging was performed with a ZEISS Axio Imager.M2 upright microscope with a ZEISS AxioCam HRm digital monochrome CCD camera for fluorescence imaging and ZEISS ApoTome.2 (Carl Zeiss Microscopy).

Campbell T.M., McSharry B.P., Steain M., Ashhurst T.M., Slobedman B, & Abendroth A. (2018). Varicella zoster virus productively infects human natural killer cells and manipulates phenotype. PLoS Pathogens, 14(4), e1006999.

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Immunolabelling of Myoblasts for Apoptosis and Wnt Signaling

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For immunolabelling, iDUX4 myoblasts were fixed in 4% paraformaldehyde/PBS for 10 min, washed in 3 X PBS for 5 min and permeabilised for 5 min with 0.5% triton X100/PBS. Subsequently, cells were blocked for 1 h using 5% goat serum/PBS (blocking buffer). Primary antibodies were added in PBS and incubated overnight at 4°C. Primary antibodies were: rabbit polyclonal anti-Cleaved-CASPASE3 (Cell Signalling; 9661S; 1:400), mouse monoclonal anti-total β-CATENIN (BD; 610154; 1:200), rabbit monoclonal anti-Non phospho(Active)β-CATENIN (1:1000, 8814, Cell Signaling), chicken polyclonal anti-GFP (Abcam; ab13970; 1:1000), and mouse monoclonal anti-DUX4 (Millipore; 9A12; 1:1000). DUX4 antibody 9A12 recognises a region common to both DUX4 and DUX4c, so was also used to detect DUX4c expression as previously described (Bosnakovski et al., 2008a (link)). Cells were then washed in 3 x PBS for 5 min, secondary antibodies added in blocking buffer and incubated for 1 h at room temperature. Secondary antibodies were: AlexaFluor 594 goat anti-mouse (Invitrogen; A11005; 1:1000) and Alexa Fluor 488 goat anti-chicken IgY (H + L) (Invitrogen; A11039; 1:1000). Nuclei were counterstained with 0.3 μM DAPI in PBS for 10 min and mounted in PBS. Cells were imaged using a classic Zeiss Axiovert 200 M epifluorescence microscope with a Zeiss AxioCam HRm and AxioVision 4.4 software (Zeiss, Jena, Germany).

Ganassi M., Figeac N., Reynaud M., Ortuste Quiroga H.P, & Zammit P.S. (2022). Antagonism Between DUX4 and DUX4c Highlights a Pathomechanism Operating Through β-Catenin in Facioscapulohumeral Muscular Dystrophy. Frontiers in Cell and Developmental Biology, 10, 802573.

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Immunofluorescence Staining of Cell Adhesion Proteins

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Deparaffinized tissue sections or 4% paraformaldehyde-fixed HaCat cells were blocked with 10% goat serum and 5% bovine serum albumin (BSA) in PBS containing 0.5% Triton X-100 (PBST) for 20 min. Staining was performed using primary antibodies against FAK (1:100 dilution), fibronectin (1:100 dilution), Ly6g (1:100 dilution), or F4/80 (1:100 dilution) at 37 °C for 3 h. For staining of integrin α5 and β1 (1:100 dilution), sections were stained at 4 °C for overnight. The slides were subsequently incubated with the appropriate fluorescent-labeled secondary antibodies (1:500 dilution) at 37 °C for 1 h and then counterstained with Hoechst 33258 for 6 min. The slide was also stained F-actin by TRITC-phalloidin (25 μg/mL) at 37 °C for 1 h. The slides were then rinsed with PBST three times, mounted with FluorSave™ reagent (Calbiochem, La Jolla, CA, USA), and viewed with an epifluorescence microscope (Zeiss Axioplan 2 imaging; Zeiss, Oberkochen, Germany) equipped with a charge-coupled device camera (Zeiss AxioCam HRm, Zeiss) and quantification was performed using Axiovert software (Zeiss AxioVision Release 4.8.2, Zeiss).

Ho T.C., Yeh S.I., Chen S.L, & Tsao Y.P. (2019). The Psoriasis Therapeutic Potential of a Novel Short Laminin Peptide C16. International Journal of Molecular Sciences, 20(13), 3144.

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Immunolabelling of Myoblast Differentiation

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For immunolabelling, immortalized myoblasts were plated at 25 000 cells/well in 96 well plates and cultured for 48 h in proliferation medium, while primary myoblasts were cultured for 24 hours, before being switched to differentiation medium for 3 days. Cells were then fixed with 4% paraformaldehyde/PBS for 15 min, washed thrice with PBS, then permeabilised with 0.1% Triton/PBS for 10 min, washed thrice again with PBS then blocked in 10% goat serum (volume/volume percent) (DakoCytomation, Glostrup, Denmark) for 30 min before being incubated on a rocker overnight at 4^oC with primary antibody against MyHC (MF-20, DSHB, IA, USA) at 1/400 in PBS supplemented with 1% goat serum
(v/v). Cells were then washed thrice with PBS before being incubated at room temperature for 30 min with AlexFluor conjugated secondary antibodies (eBioscience, Hertfordshire, UK) diluted 1:400 in PBS supplemented with 1% goat serum, washed thrice again with PBS and incubated at room temperature for 10 min in 1:1000 DAPI (4′, 6-diamidino-2-phenylindole)/PBS. Samples were imaged on a Zeiss Axiovert 200M microscope using a Zeiss AxioCam HRm and AxioVision software version 4.4 (Zeiss). At least three fields were taken at 100× magnification for each well, resulting in quantification of over 500 cells per well.

Banerji C.R., Panamarova M., Pruller J., Figeac N., Hebaishi H., Fidanis E., Saxena A., Contet J., Sacconi S., Severini S, & Zammit P.S. (2018). Dynamic transcriptomic analysis reveals suppression of PGC1α/ERRα drives perturbed myogenesis in facioscapulohumeral muscular dystrophy. Human Molecular Genetics, 28(8), 1244-1259.

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Microscopic Imaging of M. smegmatis Cells

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Single snapshot micrographs of M. smegmatis cells were captured with a Zeiss Axioskop M, Zeiss Axio.Scope, and Zeiss Axio.Observer Z1. Briefly, 2.0–5.0 μl of liquid culture was placed between a No. 1.5 glass coverslip and microscope slide. A transmitted mercury lamp light was used together with filter cubes to visualize fluorescence using a ×100 1.4 NA plan apochromatic oil immersion objective lens. Samples were located using either transmitted light, differential interference contrast, or epifluorescence. Snapshot images were captured with either a Zeiss 1 MP or Zeiss AxioCam HRm monochrome camera. Images of the same experiment were captured with the same instrument and exposure settings. Green fluorescence of EGFP was detected using the Zeiss Filter Set 38 HE. Red fluorescence of mCherry was detected using the Zeiss Filter Set 43. Images were captured using AxioVision 4.7 or ZEN Blue Microscope and Imaging Software. Images were processed using Fiji (Schindelin et al., 2012 (link)); images of the same strain were contrasted to the same maximum and minimum within an experiment.

Gessner S., Martin Z.A., Reiche M.A., Santos J.A., Dinkele R., Ramudzuli A., Dhar N., de Wet T.J., Anoosheh S., Lang D.M., Aaron J., Chew T.L., Herrmann J., Müller R., McKinney J.D., Woodgate R., Mizrahi V., Venclovas Č., Lamers M.H, & Warner D.F. (2023). Investigating the composition and recruitment of the mycobacterial ImuA′–ImuB–DnaE2 mutasome. eLife, 12, e75628.

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Tissue Sectioning and Immunostaining

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Tissues were harvested, placed in optimal cutting temperature (OCT) mounting media (Tissue-Tek, Sakura Finetek, Torrance, CA, USA). Sectioned tissues were processed as previously described with anti-VV or Anti-Active Caspase-3. Tumor images were obtained with an Epson Perfection 2450 Photo Scanner (Epson, Toronto, ON, Canada), whereas magnifications were captured using a Zeiss AxioCam HRm inverted fluorescent microscope (Zeiss, Toronto, ON, Canada) and analyzed using AxioVision 4.0 software.

Pelin A., Foloppe J., Petryk J., Singaravelu R., Hussein M., Gossart F., Jennings V.A., Stubbert L.J., Foster M., Storbeck C., Postigo A., Scut E., Laight B., Way M., Erbs P., Le Boeuf F, & Bell J.C. (2019). Deletion of Apoptosis Inhibitor F1L in Vaccinia Virus Increases Safety and Oncolysis for Cancer Therapy. Molecular Therapy Oncolytics, 14, 246-252.

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Immunofluorescence Staining of Myogenic Cells

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Myofibres and myoblasts were fixed in 4% paraformaldehyde, permeabilised in 0.5% Triton-X (Sigma-Aldrich) for 10 min, washed with PBS then blocked for 30 min in 5% (v/v) swine and goat serum (DakoCytomation Glostrup, Denmark), incubated in primary antibodies overnight at 4°C [mouse anti-DUX4 antibody [9A12 mAb, a kind gift from Alexandra Belayew (University of Mons, Mons, Belgium), 1:2000], mouse anti-Pax7 antibody [AB528428, Developmental Studies Hybridoma Bank (DSHB); 1:20–1:100], mouse anti-MyoD antibody clone 5.8A (M3512, DakoCytomation; 1:50), mouse anti-myogenin antibody (F5D, DSHB; 1:15–1:50), mouse anti-MyHC antibody (MF20, DSHB; 1:400), rabbit anti-GFP antibody (A-11122, Thermo Fisher Scientific; 1:1000), rabbit anti-phospho-histone-H1 (06-597, Millipore, 1:300) and anti-phospho-histone-H3 antibodies (06-570, Millipore; 1:100). After washing, incubation for 1 h at room temperature with Alexa-Fluor-conjugated secondary antibodies (Thermo Fisher Scientific) (Moyle and Zammit, 2014 (link)).
Images were acquired on a Zeiss Axiovert 200 M microscope using a Zeiss AxioCamHRm and AxioVision version 4.4 (Zeiss) or a Zeiss Axioplan 2 with a Hamamatsu ORCA-ER camera with Openlab 3.1.7.

Knopp P., Krom Y.D., Banerji C.R., Panamarova M., Moyle L.A., den Hamer B., van der Maarel S.M, & Zammit P.S. (2016). DUX4 induces a transcriptome more characteristic of a less-differentiated cell state and inhibits myogenesis. Journal of Cell Science, 129(20), 3816-3831.

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Microfluidic Device Perfusion Visualization

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The device was connected to microtubing at the inlet and outlet, filled with sterile water, and sealed using the clamping system. The device was then connected to a micropump (PHD Ultra, Harvard Apparatus, Holliston, MA, USA) at the inlet, which was adjusted to pump a water solution colored with a trypan blue dye (Gibco, Fisher Scientific, Waltham, MA, USA) into the device at a controlled flow rate. The flow of the colored solution within the device was then monitored using live imaging microscopy (Zeiss AxioCam HRm, Carl Zeiss, Oberkochen, Germany). The flow of the colored solution was similarly observed through collagen hydrogels previously described, which were loaded into the compartments of the LN-on-a-chip.

Shanti A., Samara B., Abdullah A., Hallfors N., Accoto D., Sapudom J., Alatoom A., Teo J., Danti S, & Stefanini C. (2020). Multi-Compartment 3D-Cultured Organ-on-a-Chip: Towards a Biomimetic Lymph Node for Drug Development. Pharmaceutics, 12(5), 464.

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Immunofluorescent Staining of Macrophages and Signaling

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Deparaffinized tissue sections or 4% PFA-fixed BMDMs were blocked with 10% goat serum and 5% BSA in PBST for 20 min. Staining was performed using primary antibodies against F4/80 (1:100 dilution) at 37 °C for 3 h. For the staining of the αv integrin (1:100 dilution) or the phospho-NF-κB p65 (1:100 dilution), sections were stained at 4 °C overnight. The slides were subsequently incubated with the appropriate fluorescent-labeled secondary antibodies (1:500 dilution) at 37 °C for 1 h and were then counterstained with Hoechst 33258 for 6 min. The slide was viewed with an epifluorescence microscope (Zeiss Axioplan 2 imaging; Zeiss, Oberkochen, Germany) equipped with a charge-coupled device camera (Zeiss AxioCamHRm, Zeiss, Germany), and quantification was performed using Axiovert software (Zeiss AxioVision Release 4.8.2, Zeiss, Germany).

Ho T.C., Yeh S.I., Chen S.L, & Tsao Y.P. (2021). Integrin αv and Vitronectin Prime Macrophage-Related Inflammation and Contribute the Development of Dry Eye Disease. International Journal of Molecular Sciences, 22(16), 8410.

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