WST-1 assay kit (Beyotime Biotechnology) was conducted to evaluate the proliferation rate of cells in our study. Briefly, transfected log-phase cells were inoculated into 96-well plates at a density of 1,000 cells per well as described before.22 (link) Then, cell proliferation rates were detected at 24 hrs, 48 hrs, 72 hrs, and 96 hrs using SpctraMax M5 (Molecular Devices, San Francisco, CA, USA).
Spctramax m5
Manufactured by Molecular Devices
Sourced in United States
The SpectraMax M5 is a multi-mode microplate reader designed for a variety of applications. It features absorbance, fluorescence, and luminescence detection capabilities, allowing for the measurement of a wide range of assays. The SpectraMax M5 provides accurate and reliable data, supporting research and development efforts in various fields.
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Lab products found in correlation
2 protocols using spctramax m5
1
Cell Proliferation Assay Protocol
2
Cell Viability Assay for CRC Cells
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The cell viability was performed using the WST-1 Assay Kit (Beyotime Biotechnology, Shanghai, China) as previously described 24 (link). Briefly, CRC cells transfected with siRNA were seeded in 96-well plates at the density of 2 × 103. After incubating for 24, 48, 72, 96 hours, 10 μl WST-1 reagent (Roche) was added into the relevant cells. SpctraMax M5 (Molecular Devices, San Francisco, CA, USA) was utilized to measure the cell proliferation rates.
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