Immunomagnetic procedure

Manufactured by Miltenyi Biotec

The immunomagnetic procedure is a laboratory technique used for the separation and isolation of specific cell populations from complex biological samples. It utilizes magnetic particles coated with antibodies that bind to target cells, allowing them to be selectively isolated from the rest of the sample using a magnetic field.

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Lab products found in correlation

Butyrate, by Merck Group (1 mentions) Fetal calf serum (fcs), by Lonza (1 mentions) Gm csf, by Merck Group (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by Merck Group (1 mentions) Immunomagnetic beads, by Miltenyi Biotec (1 mentions) Ficoll paque, by Cytiva (1 mentions) Cd138 immunomagnetic beads, by Miltenyi Biotec (1 mentions)

3 protocols using immunomagnetic procedure

Generation of Immature Dendritic Cells

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To generate DCs, CD14+ monocytes were positively selected from PBMCs of healthy donors and IgE‐mediated FA patients by immunomagnetic procedure (Miltenyi Biotec). Immature DCs were then obtained by culturing CD14+ cells at 10⁶ cells/mL in RPMI 1640 (Invitrogen), 10% FCS (Lonza), 50 ng/mL GM‐CSF, and 1000 U/mL IL‐4 with or without butyrate (Sigma‐Aldrich) as previously described.³¹

Paparo L., Nocerino R., Ciaglia E., Di Scala C., De Caro C., Russo R., Trinchese G., Aitoro R., Amoroso A., Bruno C., Di Costanzo M., Passariello A., Messina F., Agangi A., Napolitano M., Voto L., Gatta G.D., Pisapia L., Montella F., Mollica M.P., Calignano A., Puca A, & Berni Canani R. (2020). Butyrate as a bioactive human milk protective component against food allergy. Allergy, 76(5), 1398-1415.

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Differentiation of M1/M2 Macrophages with CBD

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CD14+ monocytes were positively selected from healthy donors’ and psoriatic individuals’ PBMCs by an immunomagnetic procedure (Miltenyi Biotec). Then, CD14+ cells were induced to differentiate in M1 or M2 macrophages using reagents included in the CellXVivo™ Human M1 or M2 Macrophage Differentiation Kit (R&D system), in presence of CBD (10μM) or with vehicle alone (DMSO 0,1%).

Pagano C., Ciaglia E., Coppola L., Lopardo V., Raimondo A., Giuseppe M., Lembo S., Laezza C, & Bifulco M. (2024). Cannabidiol exerts multitarget immunomodulatory effects on PBMCs from individuals with psoriasis vulgaris. Frontiers in Immunology, 15, 1373435.

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Isolation of Immune Cell Populations

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We isolated peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells by density gradient centrifugation using Ficoll–Paque (Amersham Biosciences, Uppsala, Sweden). We isolated primary MM cells from bone marrow aspirates using CD138 immunomagnetic beads (Miltenyi Biotec, Bergisch-Gladbach, Germany). Regulatory T cells were isolated from PBMCs using a two-step immunomagnetic procedure (Miltenyi). T cells were also isolated from PBMCs using negative selection with immunomagnetic beads (Miltenyi). CD8+ and CD4+ T cells were activated by anti-CD3/CD28 bead stimulation (Thermo, Waltham, MA).

García-Guerrero E., Götz R., Doose S., Sauer M., Rodríguez-Gil A., Nerreter T., Kortüm K.M., Pérez-Simón J.A., Einsele H., Hudecek M, & Danhof S. (2020). Upregulation of CD38 expression on multiple myeloma cells by novel HDAC6 inhibitors is a class effect and augments the efficacy of daratumumab. Leukemia, 35(1), 201-214.

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