The surface of the seeds untreated and after NTP treatment in all conditions was imaged using environmental scanning electron microscopy (ESEM) performed with a Quanta 450 from FEI (Thermo Fisher Scientific, Hillsboro, OR, USA). The seeds were glued using carbon double tape on the surface of aluminium stubs and then placed in the analysis chamber. The analyses were done in high vacuum conditions (~2.7 × 10−4 Pa), using 15 kV electron acceleration voltage at different magnifications.
Quanta 450
Manufactured by Thermo Fisher Scientific
Sourced in United States, Netherlands, Czechia
The Quanta 450 is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of a wide range of materials. It features a field emission gun (FEG) electron source, offering improved resolution and reduced sample charging compared to traditional tungsten filament SEMs. The Quanta 450 provides versatile imaging capabilities across a broad range of applications.
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Lab products found in correlation
Nicolet 6700, by Thermo Fisher Scientific (12 mentions)
Em cpd300, by Leica (3 mentions)
Escalab 250xi, by Thermo Fisher Scientific (3 mentions)
Tga q500, by TA Instruments (2 mentions)
D2 phaser, by Bruker (2 mentions)
Sc7620, by Quorum Technologies (2 mentions)
Raman station 400f, by PerkinElmer (2 mentions)
Oca25, by Dataphysics (2 mentions)
Jem 2100, by JEOL (2 mentions)
Sdt q600, by TA Instruments (2 mentions)
Smartlab, by Rigaku (2 mentions)
D5000 diffractometer, by Siemens (2 mentions)
Zetasizer nano z, by Malvern Panalytical (2 mentions)
Uc7 ultramicrotome, by Leica (1 mentions)
Cpd300, by Leica (1 mentions)
Skyscan 1272, by Bruker (1 mentions)
Atlas software, by TESCAN (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Tristar 3020, by Micromeritics (1 mentions)
Orius sc1000 ccd camera, by Ametek (1 mentions)
169 protocols using quanta 450
1
Surface Imaging of Seed Samples
2
Ultrastructural Analysis of Acanthella spinulosa
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Fresh A. spinulosa stems were cut to proper size and fixed in 0.1 M phosphate buffer (pH 7.4) containing 4% (v/v) glutaraldehyde for 4 h at room temperature. The samples were washed three times with 0.1 M phosphate buffer and post-fixed with 2% osmium tetroxide (w/v) plus 1.5% potassium ferricyanide (w/v) in phosphate buffer for 2 h at 4 °C. Following three rounds of water washing, in-bloc staining with 2% uranyl acetate (w/v) was performed overnight at 4 °C. The samples were dehydrated through a graded ethanol series.
For SEM observation, the samples were dried in a critical point dryer (CPD300, Leica) and imaged in a ThermoFisher Quanta 450. For TEM observation, the samples were embedded in fresh resin and polymerized at 65 °C for 24 h. Sections (70 nm) were made using a Leica UC7 ultramicrotome and post-stained with uranyl acetate and lead citrate. Grids were imaged at 80 kV in a JEOL Jem-1400 TEM using a CMOS camera (XAROSA, EMSIS). The polymerized resin block was also used for microCT (SkyScan 1272, Bruker) imaging, and the microCT data were processed using Amira (v.2020.3) software.
For SEM observation, the samples were dried in a critical point dryer (CPD300, Leica) and imaged in a ThermoFisher Quanta 450. For TEM observation, the samples were embedded in fresh resin and polymerized at 65 °C for 24 h. Sections (70 nm) were made using a Leica UC7 ultramicrotome and post-stained with uranyl acetate and lead citrate. Grids were imaged at 80 kV in a JEOL Jem-1400 TEM using a CMOS camera (XAROSA, EMSIS). The polymerized resin block was also used for microCT (SkyScan 1272, Bruker) imaging, and the microCT data were processed using Amira (v.2020.3) software.
3
Evaluating C. albicans Morphology via SEM
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C. albicans cell morphology after BrC-flav treatment was evaluated using SEM method, as we previously described [11 (link)]. An overnight culture was washed, centrifuged, suspended in PBS and incubated for 6 h in the presence of BrCl-flav at different concentrations (MIC, 2 × MIC and 5 × MIC) and DMSO as control. The samples were examined by SEM (Vega II SBH, Tescan, Brno, Czech Republic) at an acceleration voltage of 27.88 kV. Yeast to hyphal transition was investigated using a SEM system working in environmental mode (Quanta 450, FEI, Thermo Fisher Scientific, Waltham, MA, USA). The device is designed to be used with biological samples without the need to cover them with a conductive layer. The analyses were performed in low vacuum mode (at 100 Pa) in pure water vapor atmosphere with an electron acceleration voltage of 10 kV.
4
Characterization of PLGA and PLA Nanofibers
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The morphology of the PLGA and PLA nanofibrous membranes in their pristine state (ie, uncoated membranes) was studied by scanning electron microscopy (SEM). The measurements were carried out in accordance with a previously published protocol:9 (link) the membranes were sputter-coated with gold and evaluated by SEM (Quanta 450; Thermo Fisher Scientific, Waltham, MA, USA) in high vacuum mode. The images were taken using an Everhart–Thornley detector in secondary electron mode at high voltage (20 kV) and magnification 2,000× and 10,000×. The thickness of the membranes was determined from SEM images of a vertical section of the membrane. The diameter of the fibers was measured on the SEM images using Atlas software (Tescan, Brno, Czech Republic).
5
Scanning Electron Microscopy of Rice Chalkiness
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In 2020, mature rice seeds of different types of chalkiness were transversely cut using a knife and observed using a scanning electron microscope (Quanta 450; Thermo Fisher Scientific, Hillsboro, OR, USA) at an accelerating voltage in the range of 10–20 kV.
6
Fiber Morphology and Composition Analysis
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The morphology and
atomic percentage of the fibers were determined by scanning electron
microscopy (SEM) and energy-dispersive spectrometry (EDS) on a microscope
(QUANTA 450, Thermo Fisher Scientific). The fibers were coated with
gold before observing with an accelerating voltage of 10–15
kV.
atomic percentage of the fibers were determined by scanning electron
microscopy (SEM) and energy-dispersive spectrometry (EDS) on a microscope
(QUANTA 450, Thermo Fisher Scientific). The fibers were coated with
gold before observing with an accelerating voltage of 10–15
kV.
7
Scanning Electron Microscopy of Samples
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The morphology of the sample was
studied using a scanning electron
microscope (QUANTA 450, Thermo Fisher Scientific, MA). The samples
were sputtered with gold (5–10 nm thickness) and measured at
an acceleration voltage of 10–15 kV.
studied using a scanning electron
microscope (QUANTA 450, Thermo Fisher Scientific, MA). The samples
were sputtered with gold (5–10 nm thickness) and measured at
an acceleration voltage of 10–15 kV.
8
Microscopic Characterization of Chitosan Microparticles
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The size of the CS, CS-Gly, CS-LA, and CS-Gly-LA microparticles, in wet state, was determined using an optical microscope model Leica DM 750 with a built-in video camera, model ICC50 W0366, the images being recorded with 10× objective.
The surface morphology and the size of the microparticles in dry state were analyzed by Environmental Scanning Electron Microscopy (ESEM) using a Quanta 450 (Thermo Fisher Scientific, Hillsboro, Oregon, USA) microscope. The ESEM microanalysis can be performed for any samples, even non-conductive without the necessity of applying a conductive layer that can influence the surface topography. Samples were placed on an aluminum stub covered with a carbon layer, and images were achieved by applying an electron beam with an accelerating voltage of 20 kV.The diameters of the microparticles were calculated using ESEM software. All of the measurements were performed in triplicate and the values were expressed as mean ± standard deviation (SD).
The surface morphology and the size of the microparticles in dry state were analyzed by Environmental Scanning Electron Microscopy (ESEM) using a Quanta 450 (Thermo Fisher Scientific, Hillsboro, Oregon, USA) microscope. The ESEM microanalysis can be performed for any samples, even non-conductive without the necessity of applying a conductive layer that can influence the surface topography. Samples were placed on an aluminum stub covered with a carbon layer, and images were achieved by applying an electron beam with an accelerating voltage of 20 kV.The diameters of the microparticles were calculated using ESEM software. All of the measurements were performed in triplicate and the values were expressed as mean ± standard deviation (SD).
9
Morphological Characterization of Samples
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The samples were subjected to morphological characterizations using a scanning electron microscope (SEM) (Quanta 450, FEI, Thermo Fisher Scientific, Hillsboro, OR, USA) along with an energy dispersive X-ray detector (EDS) (EDAX, AMETEK Inc., Berwyn, PA, USA). The EDS spectra analysis was conducted using the TEAM version V4.1 system developed by EDAX Inc. Before the study, a calibration was performed using a standard AlCu sample consisting of a copper foil placed on an aluminum grid. The samples were analyzed in low vacuum conditions, with a pressure of about 6.1 e−4 Pa, while the acceleration voltage of the electrons was 15 kV and viewed at a magnification of 500× (20 μm).
10
Comprehensive Materials Characterization Protocol
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Scanning electron microscopy (SEM) was carried out using an FEI Quanta 450 field emission scanning electron microscope. Transmission electron microscopy (TEM) images were obtained on a JEM-2100F microscope. N2 adsorption–desorption isotherms were obtained on a Micromeritics Tristar 3020 automated surface area and pore size analyzer at −196 °C under continuous adsorption conditions. Brunauer–Emmett–Teller (BET) and Barrett–Joyner–Halenda (BJH) methods were used to determine the surface area, pore size distribution and pore volume. Dynamic light scattering (DLS) and zeta potential measurements were performed on a Malvern zeta-sizer Nano-ZS90. Fourier transform infrared (FTIR) spectra were recorded on a LAM750(s) spectrometer in transmission mode. UV–vis absorption spectra were measured on a NanoDrop 2000C spectrophotometer. Thermo-gravimetric (TG) analysis was performed on a DMA-8000 dynamic mechanical thermal analyzer at N2 atmosphere with a flow rate of 20 ml min−1 and a heating rate of 5 C° min−1.
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