Rat il 10 elisa kit

After one-month COS intervention, serum inflammatory cytokines were measured (Figure S1) by using the kits (rat IL-1β ELISA Kit, ab100768; rat IL-10 ELISA Kit, ab214566; rat IL-17A ELISA Kit, ab119536; and rat IFN-γ ELISA Kit, ab239425; Abcam, USA).

The synovial fluid samples of OA rats and the supernatant samples of RAW264.7 cells were collected. Cytokine levels were measured using the following ELISA kits, according to the manufacturer's protocol (All from Abcam, USA): Mouse IL-6 ELISA Kit, Mouse IL-1 beta ELISA Kit, Mouse TNF alpha ELISA Kit, Mouse IL-10 ELISA Kit, Rat IL-6 ELISA Kit, Rat IL-1 beta ELISA Kit, Rat TNF alpha ELISA Kit, and Rat IL-10 ELISA Kit. The results were tested using a multifunctional enzyme marker.

According to the instructions provided by the manufacturer, enzyme-linked immunosorbent assay (ELISA) kits for measuring MDA, catalase (CAT) and reduced glutathione (GSH) concentrations were acquired from Life Span Biosciences Company (LS.Bio), North America for MDA and Shanghai Blue Gene Biotech CO., LTD for CAT and GSH. CAT, GSH and MDA were measured based on methods described by [38 (link),39 (link),40 (link)], respectively. Interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) serum levels were determined utilizing particular ELISA kits (ab255730 and ab46070, consecutively) and spectrophotometric analysis in accordance with the protocols provided by the kits. Using a commercial kit purchased from Abcam, USA, IL-10 was measured (Rat IL-10 ELISA Kit, ab100765). Data from the ELISA reader was estimated and analyzed in accordance with the directions supplied with the kit.

Simple stepwise procedures were used to achieve a quantitative measurement of the serum IL-10 levels using a rat IL-10 ELISA kit, Abcam, USA. In ELISA, the sample analyte is immunocaptured in solution using an affinity tag labeled capture antibody and a reporter conjugated detecting antibody. This complete combination is then immobilized using immunoaffinity, which is provided by an anti-tag antibody that coats the well. A total of 50 μL of standards and samples were added into each well, and 50 μL of antibody cocktail was added to each well. The plate was covered with a plate covering and shaken at 450 rpm for 1 h at room temperature. Using a microplate strip washer, the plate was washed 4 times with 1× wash solution 3 times. The wells were washed again as described and filled with 100 μL of the substrate (TMB) and placed on a plate agitator before being incubated at room temperature for 10 min in the dark. Finally, 100 μL of stopping solution was added to each well to terminate the reaction. The absorbance was measured at 450 nm using Biotek ELx800 (ELISA plate reader), USA, after vigorous shaking. The IL-10 concentration was calculated by extrapolating a standard curve, and the concentration was represented in pg/mL.

The hippocampal samples were washed with cold normal saline and then homogenized on ice. The prepared homogenate was centrifuged for 10 min at 3,000 r/min to obtain supernatants. Interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interleukin-4 (IL-4), and interleukin-10 (IL-10) were detected by ELISA kits [Rat TNF alpha ELISA Kit (RAB0480) Roche, Switzerland; Rat IL-6 ELISA Kit (RAB0278) Roche, Switzerland; Rat IL-4 ELISA Kit (ab100771), Abcam; Rat IL-10 ELISA Kit (100764), Abcam]. The MDA and superoxide dismutase (SOD) activities were determined by colorimetric assay Kits. A catalase (CAT) assay kit and a ROS assay kit were used to detect the activity of CAT and the level of ROS. SOD, MDA, CAT, and ROS assay kits were purchased from Nanjing Jiancheng Bioengineering Institute. All the experimental processes were performed following the manufacturer’s instructions.

The ELISA kits for rat IL-1β and IL-6 (ab100768 and ab234570, respectively) were used with an ELISA spectrophotometer as described in the instruction manual for each kit. IL-10 was measured using a commercial kit available from Abcam co. USA (Rat IL-10 ELISA Kit (ab100765)). The levels were calculated based on data obtained from the ELISA reader according to the instructions provided for each parameter.

The level of IL-10 was determined quantitatively, a Rat IL-10 ELISA kit (Abcam, USA), was used. The ELISA technique employed a simple two-step process involving a capture antibody with an affinity tag and a detection antibody coated with a reporter. This immunocapture method allowed the measurement of IL-10 in serum samples. The quantitative measurement of IL-10 was obtained by detecting the endpoint at 450 nm using an ELISA reader, specifically the ELx800 from the USA. The IL-10 concentration in the serum was then calculated by extrapolating the data from the standard curve, and the results were expressed in picograms per milliliter (pg/mL).