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Ab65333

Manufactured by Abcam
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Sourced in United Kingdom, United States

Ab65333 is a laboratory equipment product manufactured by Abcam. It is a core component used for various scientific applications, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Insulin elisa kit, by RayBiotech (2 mentions) Ab65390, by Abcam (2 mentions) Mak052 1 kt, by Abcam (2 mentions) Mak055 1 kt, by Abcam (2 mentions) Ab102526, by Abcam (2 mentions) Glucose assay kit, by Abcam (2 mentions) Ab65330, by Abcam (2 mentions) Xf extracellular flux analyzer, by Agilent Technologies (1 mentions) Mak117, by Merck Group (1 mentions) Varioskan, by Thermo Fisher Scientific (1 mentions) Ab213477, by Abcam (1 mentions) Ab108792, by Abcam (1 mentions) Atp assay kit, by Merck Group (1 mentions) L lactate assay kit, by Abcam (1 mentions) Ab65336, by Abcam (1 mentions) Ab136957, by Abcam (1 mentions) Ab83429, by Abcam (1 mentions) Lactate dehydrogenase, by Thermo Fisher Scientific (1 mentions) Ab65331, by Abcam (1 mentions) Triton x 100, by Merck Group (1 mentions)

29 protocols using ab65333

1

Monocyte Glucose Consumption Assay

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Monocytes were cultured in a 96-well plate and treated as described above. Supernatants were collected on day 6 for glucose concentration measurement using a colorimetric glucose assay kit by Abcam (Cambridge, UK, #ab65333) according to the manufacturer’s instructions. Absorbance was measured at 570 nm with a CLARIOstar Microplate Reader and glucose consumption was calculated.
Findeisen H.M., Voges V.C., Braun L.C., Sonnenberg J., Schwarz D., Körner H., Reinecke H, & Sohrabi Y. (2022). LXRα Regulates oxLDL-Induced Trained Immunity in Macrophages. International Journal of Molecular Sciences, 23(11), 6166.
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2

Quantification of Uterine Glucose

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Glucose was quantified in samples of ULF from WT and PUGKO mice on DOPP 3, 4 and 5 (n = 5 mice per day and type) using a fluorometric glucose assay kit (ab65333; Abcam). Each sample was assayed in duplicate. The total recoverable amount of glucose was calculated by multiplying concentrations in the uterine flushing by volume of uterine flushing.
Kelleher A.M., Burns G.W., Behura S., Wu G, & Spencer T.E. (2016). Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation. Scientific Reports, 6, 38078.
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3

Metabolic Profiling of Cell Lines

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Glucose uptake and lactate excretion were measured by Abcam enzyme assay kits ab65333 and ab65331 and found to be 6.48 and 10.32 μmol per day per million cells, respectively; glycerol excretion was measured by the commercial enzyme assay kit MAK117 (Sigma-Aldrich) to be 0.14 μmol per day per million cells; glutamine and dimethyl succinate uptake and glutamate, alanine, proline and asparagine secretion were measured by LC/MS to be 1.28, 0.32, 0.6, 0.52, 0.088 and 0.08 μmol per day per million cells, respectively; and oxygen consumption was measured by a Seahorse XF Extracellular Flux Analyzer to be 4.76 μmol per day per million cells.
Liu L., Shah S., Fan J., Park J.O., Wellen K.E, & Rabinowitz J.D. (2016). Malic enzyme tracers reveal hypoxia-induced switch in adipocyte NADPH pathway usage. Nature chemical biology, 12(5), 345-352.
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4

Measuring Glucose and Insulin Levels

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The blood glucose levels were measured using a glucose assay kit (ab65333) from Abcam (Cambridge, MA, USA), and insulin secretion levels were determined using an insulin ELISA kit from RayBiotech (Norcross, GA, USA) according to the manufactory’s instruction.
Zhang Y., Liang Y., Liu H., Huang Y., Li H, & Chen B. (2020). Paeoniflorin attenuates gestational diabetes via Akt/mTOR pathway in a rat model. Food & Nutrition Research, 64, 10.29219/fnr.v64.4362.
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5

Glucose Assay and Insulin ELISA

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The blood glucose levels were measured using a glucose assay kit (ab65333) from Abcam (Cambridge, MA, USA), and insulin secretion levels were determined using an insulin ELISA kit from RayBiotech (Norcross, GA, USA) according to manufactory’s instruction.
Feng Y., Qu X., Chen Y., Feng Q., Zhang Y., Hu J, & Li X. (2020). MicroRNA-33a-5p sponges to inhibit pancreatic β-cell function in gestational diabetes mellitus LncRNA DANCR. Reproductive Biology and Endocrinology : RB&E, 18, 61.
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6

Quantifying Glucose Consumption in oxLDL-Treated Cells

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To measure glucose consumption, cells were treated with oxLDL as described above. On day 5 cells were washed once and fresh medium was applied. After 24 h glucose concentration was measured in the supernatant with a colorimetric glucose assay kit by abcam (ab65333), following the manufactures instructions. Absorbance was measured with a CLARIOstar Microplate Reader at 570 nm.
Schnack L., Sohrabi Y., Lagache S.M., Kahles F., Bruemmer D., Waltenberger J, & Findeisen H.M. (2019). Mechanisms of Trained Innate Immunity in oxLDL Primed Human Coronary Smooth Muscle Cells. Frontiers in Immunology, 10, 13.
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7

Plasma Glucose and Insulin Measurement

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Blood plasma was collected from the mouse cheek as described by Golde et al. (67 ). Plasma glucose concentration was measured using a Precision-Neo blood glucose meter (#71386-80, Abbott, Japan), plasma insulin concentration was measured using an enzyme-linked immunosorbent assay (ELISA) kit (#M1102, MORINAGA), and glucose concentration in the dialysate samples was measured using a different ELISA kit (#ab65333, Abcam), all according to the manufacturers’ instructions. Data were collected on a microplate reader (Varioskan, Thermo Fisher Scientific).
Hirai S., Miwa H., Tanaka T., Toriumi K., Kunii Y., Shimbo H., Sakamoto T., Hino M., Izumi R., Nagaoka A., Yabe H., Nakamachi T., Shioda S., Dan T., Miyata T., Nishito Y., Suzuki K., Miyashita M., Tomoda T., Hikida T., Horiuchi J., Itokawa M., Arai M, & Okado H. (2021). High-sucrose diets contribute to brain angiopathy with impaired glucose uptake and psychosis-related higher brain dysfunctions in mice. Science Advances, 7(46), eabl6077.
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8

Urinary Biomarker Measurement Protocol

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Urinary glucose and serum and urinary creatinine were measured using the colorimetric/fluorometric kits ab65333 (Abcam, Cambridge, UK) and K625 (Biovision, Milpitas, CA), respectively. Urinary cystatin C (ab201280; Abcam, Cambridge, UK), albumin (ab108792; Abcam, Cambridge, UK), and Kim-1 (ab213477; Abcam, Cambridge, UK) were measured by enzyme-linked immunosorbent assay (ELISA).
Samodelov S.L., Visentin M., Gai Z., Häusler S, & Kullak-Ublick G.A. (2019). Renal Glycosuria as a Novel Early Sign of Colistin-Induced Kidney Damage in Mice. Antimicrobial Agents and Chemotherapy, 63(12), e01650-19.
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9

Serum Glucose Quantification Assay

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Serum glucose was measured using a commercially available kit according to the manufacturer’s instructions (Abcam, Cat# ab65333). In short, 0.5–1.5 µl serum was diluted with Glucose Assay Buffer to a final volume of 50 µl and mixed in a 96-well flat bottom plate with glucose reaction mix. Following an incubation for 30 min at 37 °C in the dark the OD was measured at 570 nm in a microplate reader. Glucose concentrations were then calculated by linear regression from the standard curve.
Geiger S.S., Traba J., Richoz N., Farley T.K., Brooks S.R., Petermann F., Wang L., Gonzalez F.J., Sack M.N, & Siegel R.M. (2021). Feeding-induced resistance to acute lethal sepsis is dependent on hepatic BMAL1 and FXR signalling. Nature Communications, 12, 2745.
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10

Glucose Uptake Measurement in Cell Lines

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Briefly, the cell lines were at 60-80% confluence (exponential growth). For experiments performed after GS the cells were starved for 1 h in glucose-free medium. Then, all cell media was changed to medium containing 10 mM glucose in the presence or absence of sorafenib (10 µM). Glucose uptake was calculated by measuring the medium glucose consumption using a glucose assay kit (Abcam, ab65333) following the manufacturer’s instructions.
McGrail K., Granado-Martínez P., Esteve-Puig R., García-Ortega S., Ding Y., Sánchez-Redondo S., Ferrer B., Hernandez-Losa J., Canals F., Manzano A., Navarro-Sabaté A., Bartrons R., Yanes O., Pérez-Alea M., Muñoz-Couselo E., Garcia-Patos V, & Recio J.A. (2022). BRAF activation by metabolic stress promotes glycolysis sensitizing NRASQ61-mutated melanomas to targeted therapy. Nature Communications, 13, 7113.
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