Pd l2 clone d7u8c

Manufactured by Cell Signaling Technology

Sourced in United States

PD-L2: clone D7U8C is a primary antibody that binds to the PD-L2 protein. PD-L2 is a co-inhibitory molecule that regulates T-cell activation and immune responses. This antibody can be used for various research applications, such as flow cytometry, immunohistochemistry, and Western blotting, to study the expression and function of PD-L2 in different cell types and biological systems.

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Lab products found in correlation

Pd l1 clone e1l3n, by Cell Signaling Technology (2 mentions) Diaminobenzidine substrate system, by Nichirei Biosciences (1 mentions) Can get signal solution, by Toyobo (1 mentions) D9542, by Merck Group (1 mentions) S3023, by Agilent Technologies (1 mentions) Inform image analysis software, by PerkinElmer (1 mentions)

Close spelling variants of this product

PD-L2 (3 citations) Clone D7U8C (2 citations) D7U8C (2 citations)

3 protocols using pd l2 clone d7u8c

Immunohistochemical and FISH Analysis of PD-L1/PD-L2 in Lung Cancer

Cited 1 time

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Immunohistochemistry was performed for PD1 (clone NAT105, prediluted, CELL MARQUE, Rocklin, CA), PD-L1, PD-L2, phospho-STAT3 (PD-L1: clone E1L3N, 1:400 dilution, PD-L2: clone D7U8C, 1:20 dilution and phospho-STAT3: clone M9C6, 1:250 dilution; Cell Signaling Technology, Danvers, MA) and TTF1 (clone 8G7G3/1, prediluted, Ventana, Tucson, Arizona). Immunohistochemistry with the PD-L1 antibody was clinically validated internally against the PD-L1 22C3 clone from pharmDx and found to be comparable. Fluorescence in situ hybridization for JAK2/INSL6 and PD-L1/PD-L2 genes was performed as previously described (27 (link), 28 (link)). See supplementary methods for additional details.

Gupta S., Cheville J.C., Jungbluth A.A., Zhang Y., Zhang L., Chen Y.B., Tickoo S.K., Fine S.W., Gopalan A., Al-Ahmadie H.A., Sirintrapun S.J., Blum K.A., Lohse C.M., Hakimi A.A., Thompson R.H., Leibovich B.C., Berger M.F., Arcila M.E., Ross D.S., Ladanyi M., Antonescu C.R, & Reuter V.E. (2019). JAK2/PD-L1/PD-L2 (9p24.1) Amplifications in Renal Cell Carcinomas with Sarcomatoid Transformation: Implications for Clinical Management. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 32(9), 1344-1358.

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PD-L1/L2 Immunohistochemistry in Tissue

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Rabbit monoclonal antibodies against PD-L1 (clone E1L3N) and PD-L2 (clone D7U8C) were purchased from Cell Signaling Technology (Danvers, MA, USA). Briefly, after samples were reacted with primary antibodies, they were incubated with horseradish peroxidase (HRP)-labeled goat anti-rabbit secondary antibodies (Nichirei, Tokyo, Japan). Can Get Signal Solution (TOYOBO, Tokyo, Japan) was used to dilute the antibodies for enhancing the immunoreaction. Reactions were visualized using the diaminobenzidine substrate system (Nichirei). Two pathologists (TM and YK), who were blinded to information about the samples, evaluated the immunostaining of PD-L1/2.

Motoshima T., Komohara Y., Ma C., Dewi A.K., Noguchi H., Yamada S., Nakayama T., Kitada S., Kawano Y., Takahashi W., Sugimoto M., Takeya M., Fujimoto N., Oda Y, & Eto M. (2017). PD-L1 expression in papillary renal cell carcinoma. BMC Urology, 17, 8.

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Multiplex Immunofluorescence for PD-1, PD-L1, and PD-L2

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Multiplex immunofluorescence staining was performed according to the manufacturer's protocol. Triple staining of PD-1 (clone NAT105, Biolegend), PD-L1 (clone SP142, Genetech) and PD-L2 (clone D7U8C™, Cell Signaling Technology) was performed by the Vectra Automated Quantitative Pathology Imaging and Analysis platform through multispectral imaging system and inForm™ image analysis software from PerkinElmer's phenoptics research solution. Slides were first deparaffinized and rehydrated, followed by microwave antigen retrieval (pH = 9.0, ADI-950-274-0500, ENZO). After blocking endogenous peroxidase and nonspecific binding sites (ZAE-ICT-6295-L100, ENZO), primary Abs and secondary HRP-conjugated polymers (MPX-2402, Vectorlabs) were applied. Each HRP-conjugated polymer covalently binds a distinct fluorophore using tyramide signal amplification (Opal 7-color Fluorophore TSA plus Fluorescence Kit (NEL 797001KT; PerkinElmer)). This covalent reaction was followed by additional antigen retrieval (pH = 6.0, ADI-950-270-0500, ENZO) to remove background signal before next step. The process was conducted for the following antibodies/fluorescent dyes, in order: anti-PD-L1/Opal-570, anti-PD-1/Opal-690, anti-PD-L2/Opal-520. After three sequential reactions, slides were counterstained with DAPI (D9542, Sigma) and mounted with fluorescence mounting medium (S3023, Dako).

Ma L.J., Feng F.L., Dong L.Q., Zhang Z., Duan M., Liu L.Z., Shi J.Y., Yang L.X., Wang Z.C., Zhang S., Ding Z.B., Ke A.W., Cao Y., Zhang X.M., Zhou J., Fan J., Wang X.Y, & Gao Q. (2018). Clinical significance of PD-1/PD-Ls gene amplification and overexpression in patients with hepatocellular carcinoma. Theranostics, 8(20), 5690-5702.

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