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Bv610

Manufactured by Beckman Coulter
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The BV610 is a fluorescence-based detection system designed for use in flow cytometry applications. It provides high-sensitivity detection and quantification of fluorescently-labeled biological samples.

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Lab products found in correlation

Aqua green, by Thermo Fisher Scientific (2 mentions) Aqua green fluorescent reactive dye, by Thermo Fisher Scientific (1 mentions) Penicillin, by Euroclone (1 mentions) Streptomycin, by Euroclone (1 mentions) Duraclone im t cell subsets tube, by Beckman Coulter (1 mentions) Rpmi 1640, by Euroclone (1 mentions) Versalyse lysing solution, by Beckman Coulter (1 mentions) Dxflex, by Beckman Coulter (1 mentions)

4 protocols using bv610

1

Multiparameter Immune Responses Assessment

Cited 1 time
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SIV-specific cellular immune responses were assessed by IFN-γ ELISPOT assays and multiparameter ICS assays essentially as described4 (link). Estimates of cellular immune breadth involved IFN-γ ELISPOT assays using subpools of 10 peptides across the Gag, Pol, and Env proteins. 12-color ICS assays were performed with the Aqua green-fluorescent reactive dye (Invitrogen, L23101) and predetermined titers of mAbs (Becton-Dickinson) against CD3 (SP34; Alexa Fluor 700), CD4 (OKT4; BV711, Biolegend), CD8 (SK1; allophycocyanin-cyanine 7 [APC-Cy7]), CD28 (L293; BV610), CD95 (DX2; allophycocyanin [APC]), CD69 (TP1.55.3; phycoerythrin-Texas red [energy-coupled dye; ECD]; Beckman Coulter), gamma interferon (IFN-γ) (B27; phycoerythrin-cyanine 7 [PE-Cy7]), Ki67 (B56; fluorescein isothiocyanate [FITC]), CCR5 (3A9; phycoerythrin [PE]), CCR7(3D12; Pacific Blue), and PD-1(EH21.1; peridinin chlorophyll-A-cyanine 5.5 [PerCP-Cy5.5]). IFN-γ backgrounds were consistently <0.01% in PBMC.
Borducchi E.N., Cabral C., Stephenson K.E., Liu J., Abbink P., Ng’ang’a D., Nkolola J.P., Brinkman A.L., Peter L., Lee B.C., Jimenez J., Jetton D., Mondesir J., Mojta S., Chandrashekar A., Molloy K., Alter G., Gerold J.M., Hill A.L., Lewis M.G., Pau M.G., Schuitemaker H., Hesselgesser J., Geleziunas R., Kim J.H., Robb M.L., Michael N.L, & Barouch D.H. (2016). Ad26/MVA Therapeutic Vaccination with TLR7 Stimulation in SIV-Infected Rhesus Monkeys. Nature, 540(7632), 284-287.
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2

Multiparameter Immune Responses Assessment

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
SIV-specific cellular immune responses were assessed by IFN-γ ELISPOT assays and multiparameter ICS assays essentially as described4 (link). Estimates of cellular immune breadth involved IFN-γ ELISPOT assays using subpools of 10 peptides across the Gag, Pol, and Env proteins. 12-color ICS assays were performed with the Aqua green-fluorescent reactive dye (Invitrogen, L23101) and predetermined titers of mAbs (Becton-Dickinson) against CD3 (SP34; Alexa Fluor 700), CD4 (OKT4; BV711, Biolegend), CD8 (SK1; allophycocyanin-cyanine 7 [APC-Cy7]), CD28 (L293; BV610), CD95 (DX2; allophycocyanin [APC]), CD69 (TP1.55.3; phycoerythrin-Texas red [energy-coupled dye; ECD]; Beckman Coulter), gamma interferon (IFN-γ) (B27; phycoerythrin-cyanine 7 [PE-Cy7]), Ki67 (B56; fluorescein isothiocyanate [FITC]), CCR5 (3A9; phycoerythrin [PE]), CCR7(3D12; Pacific Blue), and PD-1(EH21.1; peridinin chlorophyll-A-cyanine 5.5 [PerCP-Cy5.5]). IFN-γ backgrounds were consistently <0.01% in PBMC.
Borducchi E.N., Cabral C., Stephenson K.E., Liu J., Abbink P., Ng’ang’a D., Nkolola J.P., Brinkman A.L., Peter L., Lee B.C., Jimenez J., Jetton D., Mondesir J., Mojta S., Chandrashekar A., Molloy K., Alter G., Gerold J.M., Hill A.L., Lewis M.G., Pau M.G., Schuitemaker H., Hesselgesser J., Geleziunas R., Kim J.H., Robb M.L., Michael N.L, & Barouch D.H. (2016). Ad26/MVA Therapeutic Vaccination with TLR7 Stimulation in SIV-Infected Rhesus Monkeys. Nature, 540(7632), 284-287.
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3

Multiparameter Flow Cytometry for SIV Immunity

Cited 1 time
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SIV-specific cellular immune responses were assessed by IFN-γ ELISPOT assays and multiparameter ICS assays essentially as previously described9 (link). 12-color ICS assays were performed with the Aqua green-fluorescent reactive dye (Invitrogen, L23101) and predetermined titers of mAbs (Becton-Dickinson) against CD3 (SP34; Alexa Fluor 700), CD4 (OKT4; BV711, Biolegend), CD8 (SK1; allophycocyanin-cyanine 7 [APC-Cy7]), CD28 (L293; BV610), CD95 (DX2; allophycocyanin [APC]), CD69 (TP1.55.3; phycoerythrin-Texas red [energy-coupled dye; ECD]; Beckman Coulter), gamma interferon (IFN-γ) (B27; phycoerythrin-cyanine 7 [PE-Cy7]), Ki67 (B56; fluorescein isothiocyanate [FITC]), CCR5 (3A9; phycoerythrin [PE]), CCR7(3D12; Pacific Blue), and PD-1(EH21.1; peridinin chlorophyll-A-cyanine 5.5 [PerCP-Cy5.5]). IFN-γ backgrounds were consistently <0.01% in PBMC.
Whitney J.B., Hill A.L., Sanisetty S., Penaloza-MacMaster P., Liu J., Shetty M., Parenteau L., Cabral C., Shields J., Blackmore S., Smith J.Y., Brinkman A.L., Peter L.E., Mathew S.I., Smith K.M., Borducchi E.N., Rosenbloom D.I., Lewis M.G., Hattersley J., Li B., Hesselgesser J., Geleziunas R., Robb M.L., Kim J.H., Michael N.L, & Barouch D.H. (2014). Rapid Seeding of the Viral Reservoir Prior to SIV Viremia in Rhesus Monkeys. Nature, 512(7512), 74-77.
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4

Comprehensive T Cell Phenotyping by Flow Cytometry

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The differentiation profiles, activation, exhaustion, and senescence of CD4+ and CD8+ T cells were analyzed by flow cytometry, using a dried reagent tube (DuraClone IM T cell subsets tube, Beckman Coulter, Hialeah, FL, USA). The DuraClone tube contained the following antibodies: CD45RA-FITC, CCR7-PE, CD28-ECD, PD1-PC5.5, CD27-PC7, CD4-APC, CD8-A700, CD3-APCA750, CD57-Pacific Blue, and CD45-Krome Orange. We added the anti-CD38 antibody BV610 (Beckman Coulter) to the Duraclone tube. Briefly, PBMC were defrosted in complete medium (RPMI-1640 supplemented with 10% fetal bovine serum, 2 mmol glutamine, 50 IU/mL penicillin, and 50 µg/mL streptomycin; EuroClone, Milan, Italy), added to the DuraClone tube and incubated for 15 min, at room temperature. After incubation, VersaLyse Lysing Solution (Beckman Coulter) was added and incubated for 15 min. Finally, samples were washed with PBS 1X, fixed with paraformaldehyde 1X, and then acquired by DxFLEX (Beckman Coulter). Samples were analyzed using DxFLEX software 2.0 (Beckman Coulter). The combined positivity to the pair of CD45RA/CCR7 immunity surface markers on CD4+ and CD8+ T cells was used to assess the percentages of naïve (CD45RA+ CCR7+), central memory (CM, CD45RA-CCR7+), effector memory (EM, CD45RA-CCR7−), and terminally differentiated effector memory (TEMRA, CD45RA+CCR7−) cells, as in [21 (link)].
Abbate I., Rozera G., Cimini E., Carletti F., Tartaglia E., Rubino M., Pittalis S., Esvan R., Gagliardini R., Mondi A., Mazzotta V., Camici M., Girardi E., Vaia F., Puro V., Antinori A, & Maggi F. (2023). Kinetics of TTV Loads in Peripheral Blood Mononuclear Cells of Early Treated Acute HIV Infections. Viruses, 15(9), 1931.
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