Uas myr gfp

The following stocks were obtained from Bloomington Drosophila Stock Center: ApplGal4 (#32040), Ddc-Gal4 (#7009), UAS-myrGFP (#32199), UAS-HTT.16Q/CyO (#33810), UAS-HTT.128Q (#33808), w¹¹¹⁸ (#5905), UAS-Hsap\SNCA.A53T (#8148), Mi{MIC}EDTP^MI08496(#44782), Df(2 R)BSC161 (#9596), P{EPgy2}EDTP^EY22967(#22600), cgGal4 (#7011), UAS-GFP-2xFYVE (#42712). UAS-Parkin-R275W/TM3 was kindly provided by Ng Chee Hoe (NNI, Singapore)41 (link), UAS-mCherry-Atg8a48 (link), hsFlp; pAct < CD2 < Gal4,UAS-nlsGFP, r4-mCherry-Atg8a40 (link); hsFlp; r4-mCherry-Atg18a; pAct < CD2 < Gal4,UAS-nlsGFP, and outcrossed Syx17^LL06330 mutants40 (link) were gifts from Gabor Juhasz (Eötvös University, Budapest, Hungary). Fly stocks were raised on standard cornmeal-sugar agar medium at 18–25 °C. L3 feeding larvae were treated for 3 hours prior to dissections. Animals were placed into a suspension consisting of instant yeast medium, supplemented by AUTEN-99 solved in DMSO (Sigma, D8418) or the same volume of DMSO only for untreated samples. For analyzing adult animals, flies were placed into vials containing treated medium immediately after eclosion and kept at 29 °C during the entire experiment. AUTEN-99 dissolved in DMSO was added to yeast suspension (final concentration was 100 or 200 μM), and dropped 65 μl to the surface of each vials. Flies were transferred into a fresh vial in every second day.

Fly stocks and crosses (unless otherwise stated) were maintained on standard cornmeal agar media at 25°C. The following strains were used: w; +; daGal4, w; gmrGal4; +, w; pplGal4; +, yw, UAS-myrGFP and QUAS-mtdTomato-3xHA; trans-Tango; + (all from Bloomington Drosophila Stock Center); w; UASTrbl-RNAi; + (Vienna Drosophila RNAi Center; VDRC_P{KK108667}VIE-260B); w; +; UAS-trbl R141Q (gift from L. Dobens, University of Missouri-Kansas City, Kansas City, MO, USA); and yw; +; ILP2-HF (gift from A. Telemans, DKFZ, Heidelberg, Germany). Haemagglutinin (HA)-tagged cDNA fragments encoding full-length trbl (ID: RH69304) from the Drosophila Genomics Resource Center were cloned into the pUASTattB vector for PhiC31-mediated site-directed transgenesis. Transgenic flies were generated at the Cambridge Fly Facility, Department of Genetics, University of Cambridge, UK. All the experiments on adult flies were performed using males.