Log In Sign up
The largest database of trusted experimental protocols

Luminex 200

Manufactured by Merck Group
Visit Supplier
Sourced in United States, Germany

The Luminex 200 is a multi-analyte profiling system designed for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded magnetic microspheres, flow cytometry, and proprietary Digital Signal Processing (DSP) technology to provide accurate and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Milliplex mouse metabolic hormone panel, by Merck Group (2 mentions) Milliplex map nonhuman primate cytokine panel, by Merck Group (2 mentions) Milliplex map kit, by Merck Group (2 mentions) Magnetic bead mapmate, by Merck Group (2 mentions) Filter bottom microtiter plates, by Merck Group (2 mentions) Detergent compatible protein quantification, by Bio-Rad (2 mentions) Milliplex software, by Merck Group (2 mentions) Bio plex manager software, by Bio-Rad (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Xponent software, by Merck Group (1 mentions) Vacuette, by Greiner (1 mentions) Modular e170, by Roche (1 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Bullet blender, by Next Advance (1 mentions) Solid phase sandwich elisa, by R&D Systems (1 mentions) Vetscan vs2, by Abaxis (1 mentions) Milliplex analyst software, by Merck Group (1 mentions) Pg lps, by InvivoGen (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Ascensia breeze 2 glucometer, by Bayer (1 mentions)

Spelling variants of this product

Luminex 200 system (71 citations) Luminex 200 analyzer (9 citations) Luminex 200 machine (8 citations) Luminex 200 3.1 xPONENT System (5 citations) Luminex 200 reader (4 citations) Luminex® 200 analyzer system (4 citations) Luminex 200 IS system (3 citations) Analyzer 3.1 Luminex 200 machine (3 citations) Luminex® 200TM System (2 citations) Luminex 200 microbead analyzer (2 citations)

61 protocols using luminex 200

1

Immune Gene Expression and Hormones in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
At months 1, 3, and 6 immune gene expression of Cohort 1 mice was measured by the nCounter GX Mouse Immunology Panel (NanoString, Seattle WA). Hormones were measured in both cohorts at all time points using the Millipore Mouse Gut Hormone Panel (Millipore Corp., St. Charles MO) using a Luminex 200 (Millipore) analyzer (see supplemental methods).
Kienesberger S., Cox L.M., Livanos A., Zhang X.S., Chung J., Perez-Perez G.I., Gorkiewicz G., Zechner E.L, & Blaser M.J. (2016). Gastric Helicobacter pylori infection affects local and distant microbial populations and host responses. Cell reports, 14(6), 1395-1407.
+ Open protocol
+ Expand
2

Measuring Glucose and Adipokines

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood glucose was measured with an Ascensia Breeze 2 glucometer (Bayer). Adipokines and insulin in plasma were measured by multiplexed ELISA using a Luminex 200 machine (Millipore).
Vernia S., Edwards Y.J., Han M.S., Cavanagh-Kyros J., Barrett T., Kim J.K, & Davis R.J. (2016). An alternative splicing program promotes adipose tissue thermogenesis. eLife, 5, e17672.
+ Open protocol
+ Expand
3

Maternal Inflammatory Biomarkers during Pregnancy

Check if the same lab product or an alternative is used in the 5 most similar protocols
The outcomes were changes in CRP (mg/L), IL-6 (pg/mL), IL-10 (ng/mL) and MMP-9 (ng/mL) serum concentrations over pregnancy and early postpartum. CRP levels (serum) were measured by the immunoturbidimetric method, MMP-9 (serum), IL-6, and IL-10 levels (plasma) were measured by xMap methodology in the Luminex 200 equipment (software xPonent/Analyst version 4.2, Millipore, St Charles, MO, USA).
Santana D.D., Kac G., dos Santos P.P., da Silva T.C., Benaim C., Cocate P.G., Trindade de Castro M.B., Heitmann B.L, & Adegboye A.R. (2022). Association between Pre-Pregnancy BMI and Inflammatory Profile Trajectories during Pregnancy and Postpartum in Brazilian Women with Periodontitis: The IMPROVE Trial. International Journal of Environmental Research and Public Health, 19(5), 2705.
+ Open protocol
+ Expand
4

Multiplex Cytokine and Bone Biomarker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multiplex assays (Milliplex®14-plex cytokine/chemokine, RANKL single-plex, and Bone single-plex detection kits, Millipore, St. Charles, MO, USA) were used to detect/quantify 16 biomarkers (eotaxin, GM-CSF, IFN-γ, IL-10, IL-12p40, IL-12p70, IL-1β, IL-2, IL-6, IL-8, IP-10, MCP-1, MIP-1α, TNF-α, RANKL, OPG) in the collected samples according to the manufacturer’s protocol. Before the assay, GCF samples were eluted from the strip in 150μL of PBS by centrifugation into a tube. Data (reported as pg/mL) was acquired on the Luminex®200™ (Millipore, St. Charles, MO) and analyzed with Luminex software (Viagene Tech, Carlisle, MA, USA), and standard-curves. Samples were analyzed by investigators (LSBA; YGM) who were blinded for the samples diagnosis as they were all coded.
Branco-de-Almeida L.S., Cruz-Almeida Y., Gonzalez-Marrero Y., Kudsi R., de Oliveira I.C., Dolia B., Huang H., Aukhil I., Harrison P, & Shaddox L.M. (2020). Treatment of localized aggressive periodontitis alters local host immunoinflammatory profiles: a long-term evaluation. Journal of clinical periodontology, 48(2), 237-248.
+ Open protocol
+ Expand
5

Murine Splenocyte Cytokine Release Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Murine splenocytes were seeded at a density of 5 million cells per well and treated with constructs at 1 × 10−6M by RNA. Cytokine release was quantified using magnetic bead assays kits from eBioscience, R&D Biosystems, or Millipore, and analyzed on a Luminex 200, all according to the manufacturer’s instructions. The dilution of cell culture supernatants (from no dilution to tenfold dilution) was conducted to ensure the measurement was within range of instrument detection.
Guan C.M., Chernyak N., Dominguez D., Cole L., Zhang B, & Mirkin C.A. (2018). RNA-Based Immunostimulatory Liposomal Spherical Nucleic Acids as Potent TLR7/8 Modulators. Small (Weinheim an der Bergstrasse, Germany), 14(49), e1803284.
+ Open protocol
+ Expand
6

Multiplex Metabolic Hormone Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fasting plasma insulin, resistin, glucagon, IL-6, TNF-alpha, and MCP-1 was measured via Milliplex Mouse metabolic hormone panel (Millipore, Burlington, MA) on the Luminex 200 instrument (Millipore).
Liu K.H., Owens J.A., Saeedi B., Cohen C.E., Bellissimo M.P., Naudin C., Darby T., Druzak S., Maner-Smith K., Orr M., Hu X., Fernandes J., Camacho M.C., Hunter-Chang S., VanInsberghe D., Ma C., Ganesh T., Yeligar S.M., Uppal K., Go Y.M., Alvarez J.A., Vos M.B., Ziegler T.R., Woodworth M.H., Kraft C.S., Jones R.M., Ortlund E., Neish A.S, & Jones D.P. (2021). Microbial metabolite delta-valerobetaine is a diet-dependent obesogen. Nature metabolism, 3(12), 1694-1705.
+ Open protocol
+ Expand
7

Serum BAFF and Cytokine Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Serum BAFF concentrations were determined by ELISA according to the manufacturer’s instruction (R&D Systems). Cytokines (GM-CSF, TNF-a, IFN-γ, IL-1B, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12, and IL-13) in plasma were measured with the High Sensitivity Human Cytokine Magnetic Bead kit (Millipore), using a Luminex 200, according to the manufacturer’s instruction.
Sng J., Ayoglu B., Chen J.W., Schickel J.N., Ferre E.M., Glauzy S., Romberg N., Hoenig M., Cunningham-Rundles C., Utz P.J., Lionakis M.S, & Meffre E. (2019). AIRE expression controls the peripheral selection of autoreactive B cells. Science immunology, 4(34), eaav6778.
+ Open protocol
+ Expand
8

Plasma Immune Markers Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
A subset of participants enrolled into RV254/SEARCH 010 completed optional procedures to facilitate cellular immunophenotyping. Plasma markers of inflammation and immune activation were quantified using either ELISA or multiplex immunoassay on a Luminex® 200 (Millipore). Data were analyzed using a 5-parameter fit logistic curve.
Paul R., Cho K., Bolzenius J., Sacdalan C., Ndhlovu L.C., Trautmann L., Krebs S., Tipsuk S., Crowell T.A., Suttichom D., Colby D.J., Premeaux T.A., Phanuphak N., Chan P., Kroon E., Vasan S., Hsu D., Carrico A., Valcour V., Ananworanich J., Robb M.L., Ake J.A., Sriplienchan S, & Spudich S. (2022). Individual Differences in CD4/CD8 T-Cell Ratio Trajectories and Associated Risk Profiles Modeled From Acute HIV Infection. Psychosomatic Medicine, 84(8), 976-983.
+ Open protocol
+ Expand
9

Chemokine Levels in Vitiligo Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral serum samples were collected from 36 patients with stable vitiligo, 13 patients with active vitiligo and 10 health controls. Thereafter, the serum levels of CXCL9 and CXCL10 were examined using the Human Chemokine Panel Kits (Millipore, Bedford, MA, USA) with Luminex 200 (Millipore). The minimum detection limits were 5 pg/ml for CXCL10 and 4 pg/ml for CXCL9.
Lin F., Hu W., Xu W., Zhou M, & Xu A. (2021). CXCL9 as a key biomarker of vitiligo activity and prediction of the success of cultured melanocyte transplantation. Scientific Reports, 11, 18298.
+ Open protocol
+ Expand
10

Multiplex Metabolic Hormone Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fasting plasma insulin, resistin, glucagon, IL-6, TNF-alpha, and MCP-1 was measured via Milliplex Mouse metabolic hormone panel (Millipore, Burlington, MA) on the Luminex 200 instrument (Millipore).
Liu K.H., Owens J.A., Saeedi B., Cohen C.E., Bellissimo M.P., Naudin C., Darby T., Druzak S., Maner-Smith K., Orr M., Hu X., Fernandes J., Camacho M.C., Hunter-Chang S., VanInsberghe D., Ma C., Ganesh T., Yeligar S.M., Uppal K., Go Y.M., Alvarez J.A., Vos M.B., Ziegler T.R., Woodworth M.H., Kraft C.S., Jones R.M., Ortlund E., Neish A.S, & Jones D.P. (2021). Microbial metabolite delta-valerobetaine is a diet-dependent obesogen. Nature metabolism, 3(12), 1694-1705.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!